##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AclI AflII AgeI AhdI ApaI
AscI AspEI AviII AvrII BbrPI
BbvCI BcgI BfrI BlnI BspHI
BssHII BssSI Bst1107I BstBI BstZ17I
CpoI DrdI Eam1105I EarI EcoRV
FspI Ksp632I KspI MfeI MluI
MunI NheI NotI NruI NspV
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacII
SapI SfiI SfuI SgrAI SmaI
SnaBI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 1055 GACGTC GACGT^C
AccI 4653 GTCGAC GT^[AC][GT]AC
AccIII 1387 TCCGGA T^CCGGA
AocI 6 CCTGAGG CC^T[ACGT]AGG
Asp700I 4020 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 302 TGGCCA TGG^CCA
BamHI 2374 GGATCC G^GATCC
BbeI 4506 GGCGCC GGCGC^C
BglI 5364 GCCCCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 3815 TACGTG [CT]AC^GT[GA]
BsaBI 5105 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1387 TCCGGA T^CCGGA
BspEI 1387 TCCGGA T^CCGGA
BsrBI 3933 GAGCGG CCGCTC(-3/-3)
BstEII 351 GGTGACC G^GT[ACGT]ACC
BstPI 351 GGTGACC G^GT[ACGT]ACC
BstXI 3109 CCAGCACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 6 CCTGAGG CC^T[ACGT]AGG
EagI 2172 CGGCCG C^GGCCG
EclXI 2172 CGGCCG C^GGCCG
Eco52I 2172 CGGCCG C^GGCCG
Eco81I 6 CCTGAGG CC^T[ACGT]AGG
EcoO65I 351 GGTGACC G^GT[ACGT]ACC
FseI 2184 GGCCGGCC GGCCGG^CC
KasI 4506 GGCGCC G^GCGCC
MamI 5105 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 302 TGGCCA TGG^CCA
MroI 1387 TCCGGA T^CCGGA
MscI 302 TGGCCA TGG^CCA
NarI 4506 GGCGCC GG^CGCC
PvuII 4675 CAGCTG CAG^CTG
SacI 4667 GAGCTC GAGCT^C
SalI 4653 GTCGAC G^TCGAC
SbfI 2865 CCTGCAGG CCTGCA^GG
ScaI 515 AGTACT AGT^ACT
SexAI 5137 ACCTGGT A^CC[AT]GGT
SfoI 4506 GGCGCC GGC^GCC
SpeI 4191 ACTAGT A^CTAGT
Sse8387I 2865 CCTGCAGG CCTGCA^GG
SwaI 4203 ATTTAAAT ATTT^AAAT
XbaI 2669 TCTAGA T^CTAGA
XmnI 4020 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 182 GGTACC G^GTACC
3875 GGTACC
Alw44I 3086 GTGCAC G^TGCAC
3818 GTGCAC
ApaLI 3086 GTGCAC G^TGCAC
3818 GTGCAC
Asp718I 182 GGTACC G^GTACC
3875 GGTACC
AspI 1127 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1580 GACACAGTC
BciVI 1256 GTATCC GTATCC(6/5)
5322 GTATCC
BclI 50 TGATCA T^GATCA
1114 TGATCA
BglII 684 AGATCT A^GATCT
3741 AGATCT
BlpI 647 GCTCAGC GC^T[ACGT]AGC
4219 GCTAAGC
Bpu1102I 647 GCTCAGC GC^T[ACGT]AGC
4219 GCTAAGC
BsaI 2161 GAGACC GGTCTC(1/5)
1778 GGTCTC
BsiWI 1825 CGTACG C^GTACG
2263 CGTACG
BsmI 368 GAATGC GAATGC(1/-1)
2578 GAATGC
BspDI 1369 ATCGAT AT^CGAT
2429 ATCGAT
BstAPI 255 GCAAGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4691 GCAGCGCGTGC
CelII 647 GCTCAGC GC^T[ACGT]AGC
4219 GCTAAGC
ClaI 1369 ATCGAT AT^CGAT
2429 ATCGAT
EcoRI 534 GAATTC G^AATTC
1529 GAATTC
FbaI 50 TGATCA T^GATCA
1114 TGATCA
HindIII 1616 AAGCTT A^AGCTT
5528 AAGCTT
HpaI 2294 GTTAAC GTT^AAC
5010 GTTAAC
KpnI 182 GGTACC GGTAC^C
3875 GGTACC
NcoI 2203 CCATGG C^CATGG
3606 CCATGG
NdeI 1447 CATATG CA^TATG
3864 CATATG
PaeR7I 4459 CTCGAG C^TCGAG
5485 CTCGAG
PflFI 1127 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1580 GACACAGTC
SphI 1298 GCATGC GCATG^C
5348 GCATGC
Tth111I 1127 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1580 GACACAGTC
XcmI 308 CCACGCAGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4548 CCAGTGAGTCCTTGG
XhoI 4459 CTCGAG C^TCGAG
5485 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI Aor51HI
ApoI AseI AsnI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspLU11I BspMI BsrDI BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI Eco47III EcoNI EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NsiI
NspI PacI PflMI PleI PpuMI
PshBI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII