##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AclI AgeI AocI AscI AseI
AsnI AvrII BamHI BclI BglII
BlnI BsaBI BsiWI BsmI Bsp1407I
BspDI BspLU11I BsrGI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI Eco81I EcoO65I EcoRV
FbaI FseI HincII HindII HindIII
HpaI MamI MfeI MunI NheI
NotI NruI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI ScaI SfiI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 2036 GGTACC G^GTACC
AccIII 2202 TCCGGA T^CCGGA
AcsI 3488 GAATTC [GA]^AATT[CT]
AflII 485 CTTAAG C^TTAAG
AflIII 2497 ACGCGT A^C[GA][CT]GT
AhdI 1062 GACAGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 3488 GAATTC [GA]^AATT[CT]
Asp700I 3902 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2036 GGTACC G^GTACC
AspEI 1062 GACAGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 419 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1715 CACGTG CAC^GTG
BciVI 1531 GGATAC GTATCC(6/5)
BfrI 485 CTTAAG C^TTAAG
BseAI 2202 TCCGGA T^CCGGA
BspEI 2202 TCCGGA T^CCGGA
BspHI 114 TCATGA T^CATGA
BsrDI 637 CATTGC GCAATG(2/0)
BssHII 1386 GCGCGC G^CGCGC
BstYI 1557 AGATCC [GA]^GATC[CT]
DrdI 3622 GACTTCGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1062 GACAGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3488 GAATTC G^AATTC
KpnI 2036 GGTACC GGTAC^C
KspI 530 CCGCGG CCGC^GG
MflI 1557 AGATCC [GA]^GATC[CT]
MluI 2497 ACGCGT A^CGCGT
MroI 2202 TCCGGA T^CCGGA
MslI 1489 CACCTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 813 CCATGG C^CATGG
NdeI 1628 CATATG CA^TATG
NspI 1086 ACATGC [GA]CATG^[CT]
PaeR7I 802 CTCGAG C^TCGAG
PflFI 419 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1715 CACGTG CAC^GTG
PmlI 1715 CACGTG CAC^GTG
RcaI 114 TCATGA T^CATGA
SacII 530 CCGCGG CCGC^GG
SapI 1813 GAAGAGC GCTCTTC(1/4)
SbfI 3468 CCTGCAGG CCTGCA^GG
Sse8387I 3468 CCTGCAGG CCTGCA^GG
Tsp509I 3489 AATT ^AATT
Tth111I 419 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 802 CTCGAG C^TCGAG
XhoII 1557 AGATCC [GA]^GATC[CT]
XmnI 3902 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 2249 GTCTAC GT^[AC][GT]AC
3113 GTCTAC
AlwI 1558 GATCC GGATC(4/5)
2832 GGATC
AviII 1293 TGCGCA TGC^GCA
2457 TGCGCA
BbeI 1639 GGCGCC GGCGC^C
3643 GGCGCC
BcgI 3673 CGAGGGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2880 GCATCGTGTTCG
BlpI 3723 GCTCAGC GC^T[ACGT]AGC
3728 GCTGAGC
Bpu1102I 3723 GCTCAGC GC^T[ACGT]AGC
3728 GCTGAGC
BsaAI 1313 TACGTG [CT]AC^GT[GA]
1715 CACGTG
BsaWI 360 ACCGGA [AT]^CCGG[AT]
2202 TCCGGA
BsiEI 73 CGGCCG CG[GA][CT]^CG
1844 CGGCCG
BstAPI 2806 GCAGTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3854 GCAGGCCCTGC
CelII 3723 GCTCAGC GC^T[ACGT]AGC
3728 GCTGAGC
DraIII 4010 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
4062 CACCGTGTG
EagI 73 CGGCCG C^GGCCG
1844 CGGCCG
EarI 1813 GAAGAG CTCTTC(1/4)
2273 GAAGAG
EclXI 73 CGGCCG C^GGCCG
1844 CGGCCG
Eco52I 73 CGGCCG C^GGCCG
1844 CGGCCG
EcoT22I 569 ATGCAT ATGCA^T
1625 ATGCAT
FspI 1293 TGCGCA TGC^GCA
2457 TGCGCA
KasI 1639 GGCGCC G^GCGCC
3643 GGCGCC
Ksp632I 1813 GAAGAG CTCTTC(1/4)
2273 GAAGAG
NarI 1639 GGCGCC GG^CGCC
3643 GGCGCC
NsiI 569 ATGCAT ATGCA^T
1625 ATGCAT
SexAI 1929 ACCAGGT A^CC[AT]GGT
4011 ACCAGGT
SfoI 1639 GGCGCC GGC^GCC
3643 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AluI Alw44I AlwNI Aor51HI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI Eco47III EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I
Van91I XcmI XmaCI XmaI