##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AhdI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BbrPI
BfaI BfrI BlnI BsaI BsiWI
BsmI Bsp1407I BspLU11I BsrGI BssHII
Bst1107I BstBI BstZ17I CpoI DraI
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV EcoT22I FseI FspI HindIII
HpaI KpnI KspI MaeI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I RsrII SacII SalI SbfI
SexAI SfiI SfuI SmaI SnaBI
SpeI Sse8387I SspBI StuI SwaI
XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 2563 GACGTC GACGT^C
AccIII 447 TCCGGA T^CCGGA
AocI 3883 CCTGAGG CC^T[ACGT]AGG
AspI 3335 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 68 GGCGCC GGCGC^C
BciVI 4035 GTATCC GTATCC(6/5)
BglI 3717 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 3007 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 447 TCCGGA [AT]^CCGG[AT]
BseAI 447 TCCGGA T^CCGGA
BsiEI 1102 CGATCG CG[GA][CT]^CG
BspEI 447 TCCGGA T^CCGGA
BspHI 3504 TCATGA T^CATGA
BsrDI 1462 CATTGC GCAATG(2/0)
Bsu36I 3883 CCTGAGG CC^T[ACGT]AGG
DraIII 4101 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 85 GACAGAAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3883 CCTGAGG CC^T[ACGT]AGG
EcoNI 1948 CCTTGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI 68 GGCGCC G^GCGCC
MamI 3007 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 447 TCCGGA T^CCGGA
NarI 68 GGCGCC GG^CGCC
PaeR7I 885 CTCGAG C^TCGAG
PflFI 3335 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 1102 CGATCG CGAT^CG
RcaI 3504 TCATGA T^CATGA
SapI 1589 GAAGAGC GCTCTTC(1/4)
ScaI 915 AGTACT AGT^ACT
SfoI 68 GGCGCC GGC^GCC
SgrAI 64 CGCCGGCG C[GA]^CCGG[CT]G
SphI 1278 GCATGC GCATG^C
SspI 2552 AATATT AAT^ATT
Tth111I 3335 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 885 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 772 GTCTAC GT^[AC][GT]AC
3736 GTCTAC
Alw44I 1361 GTGCAC G^TGCAC
2336 GTGCAC
Aor51HI 1260 AGCGCT AGC^GCT
2103 AGCGCT
ApaLI 1361 GTGCAC G^TGCAC
2336 GTGCAC
BamHI 2028 GGATCC G^GATCC
2919 GGATCC
BanI 68 GGCGCC G^G[CT][GA]CC
1372 GGTGCC
BcgI 1096 GCAAGCCGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1782 GCATCGTGATCG
BclI 2007 TGATCA T^GATCA
3118 TGATCA
BglII 2658 AGATCT A^GATCT
3744 AGATCT
BlpI 1229 GCTCAGC GC^T[ACGT]AGC
1523 GCTCAGC
Bpu1102I 1229 GCTCAGC GC^T[ACGT]AGC
1523 GCTCAGC
BspDI 168 ATCGAT AT^CGAT
2597 ATCGAT
BsrFI 65 GCCGGC [GA]^CCGG[CT]
2705 GCCGGC
BstEII 425 GGTGACC G^GT[ACGT]ACC
2398 GGTGACC
BstPI 425 GGTGACC G^GT[ACGT]ACC
2398 GGTGACC
CelII 1229 GCTCAGC GC^T[ACGT]AGC
1523 GCTCAGC
Cfr10I 65 GCCGGC [GA]^CCGG[CT]
2705 GCCGGC
ClaI 168 ATCGAT AT^CGAT
2597 ATCGAT
DsaI 3648 CCATGG C^C[GA][CT]GG
3780 CCATGG
Eco47III 1260 AGCGCT AGC^GCT
2103 AGCGCT
EcoO65I 425 GGTGACC G^GT[ACGT]ACC
2398 GGTGACC
FbaI 2007 TGATCA T^GATCA
3118 TGATCA
HincII 2267 GTTGAC GT[CT]^[GA]AC
2576 GTTGAC
HindII 2267 GTTGAC GT[CT]^[GA]AC
2576 GTTGAC
MseI 2618 TTAA T^TAA
4139 TTAA
NaeI 65 GCCGGC GCC^GGC
2705 GCCGGC
NcoI 3648 CCATGG C^CATGG
3780 CCATGG
NgoMIV 65 GCCGGC G^CCGGC
2705 GCCGGC
SacI 2765 GAGCTC GAGCT^C
3270 GAGCTC
Tru9I 2618 TTAA T^TAA
4139 TTAA
XcmI 1443 CCACTTCTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2634 CCAGGATTGGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BanII
BbsI BbvCI BbvI BcnI BmyI
BpmI BpuAI BsaAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII