##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccIII AflII AflIII
AgeI Alw44I AocI Aor51HI ApaLI
AscI Asp718I AvrII BamHI BbrPI
BcgI BfrI BglI BglII BlnI
BsaAI BsaBI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraIII EagI EclXI
Eco47III Eco52I Eco81I EcoT14I FseI
HpaI KpnI MamI MluI MroI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PvuI RsrII
SalI SbfI SfiI SfuI SgrAI
SmaI SnaBI Sse8387I SspBI StuI
StyI SwaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccII 5389 CGCG CG^CG
AclI 1104 AACGTT AA^CGTT
ApaI 3622 GGGCCC GGGCC^C
AseI 2661 ATTAAT AT^TAAT
AsnI 2661 ATTAAT AT^TAAT
AspHI 2153 GAGCTC G[AT]GC[AT]^C
AspI 5185 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3986 TGCGCA TGC^GCA
BalI 1564 TGGCCA TGG^CCA
BciVI 6010 GTATCC GTATCC(6/5)
BclI 5650 TGATCA T^GATCA
BsaWI 2260 ACCGGA [AT]^CCGG[AT]
BsiHKAI 2153 GAGCTC G[AT]GC[AT]^C
BsmI 2991 GAATGC GAATGC(1/-1)
BstAPI 4934 GCAGGTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 5389 CGCG CG^CG
BstXI 5278 CCAATGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 5155 GACTACTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1564 TGGCCA [CT]^GGCC[GA]
EcoT22I 425 ATGCAT ATGCA^T
FauI 4790 CCCGC CCCGC(4/6)
FbaI 5650 TGATCA T^GATCA
FspI 3986 TGCGCA TGC^GCA
HindIII 3693 AAGCTT A^AGCTT
KspI 5388 CCGCGG CCGC^GG
MfeI 1093 CAATTG C^AATTG
MluNI 1564 TGGCCA TGG^CCA
MscI 1564 TGGCCA TGG^CCA
MunI 1093 CAATTG C^AATTG
MvnI 5389 CGCG CG^CG
NsiI 425 ATGCAT ATGCA^T
PaeR7I 5783 CTCGAG C^TCGAG
PflFI 5185 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3573 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2661 ATTAAT AT^TAAT
Psp1406I 1104 AACGTT AA^CGTT
SacI 2153 GAGCTC GAGCT^C
SacII 5388 CCGCGG CCGC^GG
SexAI 3196 ACCAGGT A^CC[AT]GGT
Tth111I 5185 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 5783 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwNI 2016 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
4363 CAGCCCCTG
AvaI 2116 CTCGGG C^[CT]CG[GA]G
5783 CTCGAG
BcnI 2092 CCCGG CC^[GC]GG
4012 CCGGG
BlpI 1935 GCTGAGC GC^T[ACGT]AGC
3183 GCTGAGC
Bpu1102I 1935 GCTGAGC GC^T[ACGT]AGC
3183 GCTGAGC
BsgI 674 GTGCAG GTGCAG(16/14)
1729 GTGCAG
BsoBI 2116 CTCGGG C^[CT]CG[GA]G
5783 CTCGAG
BspHI 2945 TCATGA T^CATGA
4469 TCATGA
BstEII 1509 GGTGACC G^GT[ACGT]ACC
3380 GGTAACC
BstPI 1509 GGTGACC G^GT[ACGT]ACC
3380 GGTAACC
CelII 1935 GCTGAGC GC^T[ACGT]AGC
3183 GCTGAGC
EcoO65I 1509 GGTGACC G^GT[ACGT]ACC
3380 GGTAACC
EcoRI 434 GAATTC G^AATTC
1056 GAATTC
EcoRV 843 GATATC GAT^ATC
6029 GATATC
HgaI 11 GACGC GACGC(5/10)
5189 GCGTC
NciI 2092 CCCGG CC^[GC]GG
4012 CCGGG
PflMI 1556 CCAGGGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2360 CCAGTTTGTGG
PstI 2022 CTGCAG CTGCA^G
3822 CTGCAG
RcaI 2945 TCATGA T^CATGA
4469 TCATGA
SapI 813 GCTCTTC GCTCTTC(1/4)
6059 GCTCTTC
SpeI 3869 ACTAGT A^CTAGT
6206 ACTAGT
Van91I 1556 CCAGGGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2360 CCAGTTTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AhdI AluI AlwI ApoI Asp700I
AspEI AvaII BanI BanII BbeI
BbsI BbvCI BbvI BfaI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI Eam1105I EarI EcoNI EcoO109I
EcoRII Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NarI NdeII NlaIII NlaIV NspI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SfoI SmlI SphI SspI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XhoII XmnI