##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AccI AflII AgeI AhdI AscI
AseI AsnI AspEI AvrII BamHI
BclI BfrI BlnI BsiWI BsrFI
BssHII Bst1107I BstBI BstZ17I Cfr10I
CpoI EagI Eam1105I EclXI Eco52I
EcoRV FbaI FseI MfeI MluI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshBI PvuI RsrII
SalI SapI SbfI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 3169 GACGTC GACGT^C
Acc65I 1467 GGTACC G^GTACC
AccIII 2277 TCCGGA T^CCGGA
AclI 23 AACGTT AA^CGTT
Alw44I 368 GTGCAC G^TGCAC
ApaLI 368 GTGCAC G^TGCAC
Asp718I 1467 GGTACC G^GTACC
AviII 2388 TGCGCA TGC^GCA
BciVI 1285 GGATAC GTATCC(6/5)
BglI 2360 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 2365 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2277 TCCGGA T^CCGGA
BsiEI 3832 CGGTCG CG[GA][CT]^CG
Bsp1407I 1443 TGTACA T^GTACA
BspDI 611 ATCGAT AT^CGAT
BspEI 2277 TCCGGA T^CCGGA
BspHI 4451 TCATGA T^CATGA
BsrGI 1443 TGTACA T^GTACA
BstEII 1792 GGTCACC G^GT[ACGT]ACC
BstPI 1792 GGTCACC G^GT[ACGT]ACC
ClaI 611 ATCGAT AT^CGAT
DraI 4374 TTTAAA TTT^AAA
DraIII 3239 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1792 GGTCACC G^GT[ACGT]ACC
EcoRI 5058 GAATTC G^AATTC
EcoT22I 2740 ATGCAT ATGCA^T
FspI 2388 TGCGCA TGC^GCA
HincII 3865 GTTAAC GT[CT]^[GA]AC
HindII 3865 GTTAAC GT[CT]^[GA]AC
HpaI 3865 GTTAAC GTT^AAC
KpnI 1467 GGTACC GGTAC^C
KspI 2973 CCGCGG CCGC^GG
MamI 2365 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2277 TCCGGA T^CCGGA
NcoI 1989 CCATGG C^CATGG
NsiI 2740 ATGCAT ATGCA^T
PshAI 3487 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 23 AACGTT AA^CGTT
RcaI 4451 TCATGA T^CATGA
SacII 2973 CCGCGG CCGC^GG
SphI 2742 GCATGC GCATG^C
SspBI 1443 TGTACA T^GTACA
StuI 3422 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 2889 CGCG CG^CG
2974 CGCG
AflIII 2562 ACATGT A^C[GA][CT]GT
4099 ACATGT
Aor51HI 2980 AGCGCT AGC^GCT
3814 AGCGCT
ApaI 2178 GGGCCC GGGCC^C
2690 GGGCCC
Asp700I 2560 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5058 GAATTCCTTC
BbeI 1725 GGCGCC GGCGC^C
3791 GGCGCC
BcgI 1920 GCAAATCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4575 GCAGGAGAATCG
BglII 788 AGATCT A^GATCT
2448 AGATCT
BsmBI 847 CGTCTC CGTCTC(1/5)
4665 CGTCTC
BspLU11I 2562 ACATGT A^CATGT
4099 ACATGT
BstUI 2889 CGCG CG^CG
2974 CGCG
DrdI 539 GACGCTCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3262 GACATGCGGGTC
Eco47III 2980 AGCGCT AGC^GCT
3814 AGCGCT
KasI 1725 GGCGCC G^GCGCC
3791 GGCGCC
MvnI 2889 CGCG CG^CG
2974 CGCG
NarI 1725 GGCGCC GG^CGCC
3791 GGCGCC
PflMI 189 CCAACGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
803 CCACTCTCTGG
SexAI 3240 ACCAGGT A^CC[AT]GGT
5134 ACCAGGT
SfoI 1725 GGCGCC GGC^GCC
3791 GGCGCC
SspI 3665 AATATT AAT^ATT
5180 AATATT
TaqI 612 TCGA T^CGA
1617 TCGA
TthHB8I 612 TCGA T^CGA
1617 TCGA
Van91I 189 CCAACGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
803 CCACTCTCTGG
XmnI 2560 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5058 GAATTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AspI AvaI AvaII BalI BanI
BanII BbrPI BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflFI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I XcmI
XhoII