##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AhdI Alw44I ApaLI AscI Asp718I
AspEI AspI AviII AvrII BbrPI
BcgI BclI BfrI BlnI BsaAI
BsaBI BsaI BseAI BspDI BspEI
BsrDI Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI DrdI Eam1105I EcoRI
EcoRV FbaI FseI FspI HpaI
KpnI MamI MluI MroI NheI
NotI NspV PacI PflFI PmaCI
PmeI PmlI PshAI PvuI RsrII
SalI SapI SbfI SfuI SgrAI
SnaBI SphI Sse8387I SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AclI 3467 AACGTT AA^CGTT
AgeI 846 ACCGGT A^CCGGT
AocI 4778 CCTTAGG CC^T[ACGT]AGG
AseI 2655 ATTAAT AT^TAAT
AsnI 2655 ATTAAT AT^TAAT
BbvCI 2944 GCTGAGG CCTCAGC(-5/-2)
BglII 2564 AGATCT A^GATCT
BlpI 764 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 764 GCTGAGC GC^T[ACGT]AGC
BsaWI 846 ACCGGT [AT]^CCGG[AT]
BsiWI 4010 CGTACG C^GTACG
BsmBI 2355 CGTCTC CGTCTC(1/5)
BsmI 3024 GCATTC GAATGC(1/-1)
BspHI 2833 TCATGA T^CATGA
BssSI 515 CACGAG CACGAG(-5/-1)
Bsu36I 4778 CCTTAGG CC^T[ACGT]AGG
CelII 764 GCTGAGC GC^T[ACGT]AGC
DraIII 1661 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 4778 CCTTAGG CC^T[ACGT]AGG
HincII 1768 GTTGAC GT[CT]^[GA]AC
HindII 1768 GTTGAC GT[CT]^[GA]AC
HindIII 4202 AAGCTT A^AGCTT
MfeI 1994 CAATTG C^AATTG
MunI 1994 CAATTG C^AATTG
NcoI 1393 CCATGG C^CATGG
NruI 5006 TCGCGA TCG^CGA
PaeR7I 1443 CTCGAG C^TCGAG
PinAI 846 ACCGGT A^CCGGT
PpuMI 914 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 2655 ATTAAT AT^TAAT
Psp1406I 3467 AACGTT AA^CGTT
PstI 2192 CTGCAG CTGCA^G
RcaI 2833 TCATGA T^CATGA
ScaI 4048 AGTACT AGT^ACT
SexAI 3932 ACCAGGT A^CC[AT]GGT
SfiI 484 GGCCGAGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 2475 ACTAGT A^CTAGT
StuI 2708 AGGCCT AGG^CCT
XcmI 1058 CCAGGCATCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1443 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwNI 159 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1244 CAGCGACTG
Aor51HI 23 AGCGCT AGC^GCT
1486 AGCGCT
BalI 1326 TGGCCA TGG^CCA
1802 TGGCCA
BciVI 2609 GGATAC GTATCC(6/5)
4311 GGATAC
BsgI 4069 CTGCAC GTGCAG(16/14)
4908 CTGCAC
BsrBI 89 GAGCGG CCGCTC(-3/-3)
635 GAGCGG
BssHII 314 GCGCGC G^CGCGC
646 GCGCGC
BstEII 1676 GGTGACC G^GT[ACGT]ACC
3525 GGTCACC
BstPI 1676 GGTGACC G^GT[ACGT]ACC
3525 GGTCACC
BstXI 2076 CCAGGGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2722 CCAGAGCTCTGG
Eco47III 23 AGCGCT AGC^GCT
1486 AGCGCT
EcoO65I 1676 GGTGACC G^GT[ACGT]ACC
3525 GGTCACC
EcoT22I 2390 ATGCAT ATGCA^T
2551 ATGCAT
MluNI 1326 TGGCCA TGG^CCA
1802 TGGCCA
MscI 1326 TGGCCA TGG^CCA
1802 TGGCCA
NsiI 2390 ATGCAT ATGCA^T
2551 ATGCAT
SmlI 1443 CTCGAG C^T[CT][GA]AG
2874 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI ApaI ApoI
Asp700I AspHI AvaI AvaII BamHI
BanI BanII BbeI BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspLU11I BspMI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PvuII RsaI
SacI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmaCI XmaI XmnI