##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccIII AclI AcyI AhdI
AocI ApaI AscI AspEI AspI
BbeI BbrPI BclI BglII BlpI
Bpu1102I BsaHI BseAI BsiWI BsmBI
BspDI BspEI BssHII BssSI BstAPI
BstBI BstEII BstPI Bsu36I CelII
ClaI CpoI Eam1105I Eco81I EcoO65I
FbaI FseI Hin1I KasI KspI
MluI MroI NaeI NarI NgoMIV
NheI NotI NruI NspV PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PshAI Psp1406I PvuI PvuII RsrII
SacII SalI SapI SbfI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I StuI Tth111I Van91I XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 5978 GGTACC G^GTACC
AccI 4065 GTATAC GT^[AC][GT]AC
AccII 173 CGCG CG^CG
AgeI 1919 ACCGGT A^CCGGT
Aor51HI 7 AGCGCT AGC^GCT
Asp718I 5978 GGTACC G^GTACC
AviII 4673 TGCGCA TGC^GCA
BanII 4848 GAGCTC G[GA]GC[CT]^C
BcgI 5583 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2588 GGATAC GTATCC(6/5)
BsaBI 2184 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1919 ACCGGT [AT]^CCGG[AT]
Bsp1407I 1563 TGTACA T^GTACA
BspMI 5975 GCAGGT ACCTGC(4/8)
BsrFI 1919 ACCGGT [GA]^CCGG[CT]
BsrGI 1563 TGTACA T^GTACA
Bst1107I 4065 GTATAC GTA^TAC
BstUI 173 CGCG CG^CG
BstZ17I 4065 GTATAC GTA^TAC
Cfr10I 1919 ACCGGT [GA]^CCGG[CT]
DrdI 440 GACGCTTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 7 AGCGCT AGC^GCT
EcoNI 243 CCTTCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2090 GAATTC G^AATTC
EcoRV 5915 GATATC GAT^ATC
FspI 4673 TGCGCA TGC^GCA
HaeII 7 AGCGCT [GA]GCGC^[CT]
KpnI 5978 GGTACC GGTAC^C
MamI 2184 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 173 CGCG CG^CG
NcoI 6037 CCATGG C^CATGG
PacI 1090 TTAATTAA TTAAT^TAA
PinAI 1919 ACCGGT A^CCGGT
SacI 4848 GAGCTC GAGCT^C
SexAI 6105 ACCAGGT A^CC[AT]GGT
SfiI 58 GGCCTGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 1563 TGTACA T^GTACA
SwaI 843 ATTTAAAT ATTT^AAAT
XbaI 5919 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflII 1032 CTTAAG C^TTAAG
4461 CTTAAG
BamHI 311 GGATCC G^GATCC
1129 GGATCC
BbvCI 5594 CCTCAGC CCTCAGC(-5/-2)
2042 GCTGAGG
BfrI 1032 CTTAAG C^TTAAG
4461 CTTAAG
BglI 59 GCCTGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
170 GCCCGCGTGGC
BsaAI 1270 CACGTA [CT]AC^GT[GA]
6215 TACGTG
BsiEI 149 CGGCCG CG[GA][CT]^CG
293 CGGCCG
BsrBI 366 CCGCTC CCGCTC(-3/-3)
384 CCGCTC
BstXI 2578 CCAGATAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3166 CCATTTTTATGG
DraIII 4289 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
4330 CACAGGGTG
DsaI 296 CCGTGG C^C[GA][CT]GG
6037 CCATGG
EagI 149 CGGCCG C^GGCCG
293 CGGCCG
EarI 1302 CTCTTC CTCTTC(1/4)
4792 CTCTTC
EclXI 149 CGGCCG C^GGCCG
293 CGGCCG
Eco52I 149 CGGCCG C^GGCCG
293 CGGCCG
EcoT22I 3566 ATGCAT ATGCA^T
4446 ATGCAT
FauI 171 CCCGC CCCGC(4/6)
371 CCCGC
Ksp632I 1302 CTCTTC CTCTTC(1/4)
4792 CTCTTC
NdeI 4162 CATATG CA^TATG
4688 CATATG
NsiI 3566 ATGCAT ATGCA^T
4446 ATGCAT
PpuMI 2050 AGGACCT [GA]G^G[AT]CC[CT]
5459 AGGACCT
SmaI 22 CCCGGG CCC^GGG
5571 CCCGGG
SmlI 1032 CTTAAG C^T[CT][GA]AG
4461 CTTAAG
XcmI 1317 CCAGTATAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1985 CCACTGAAGAAGTGG
XmaCI 22 CCCGGG C^CCGGG
5571 CCCGGG
XmaI 22 CCCGGG C^CCGGG
5571 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI AlwNI ApaLI ApoI
AseI AsnI Asp700I AspHI AvaI
AvaII AvrII BalI BanI BbsI
BbvI BcnI BfaI BlnI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspLU11I BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HindIII HinfI HpaI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MfeI MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MunI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PshBI PstI RcaI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI