##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII Acc65I AccI AgeI AhdI
Aor51HI AscI Asp718I AspEI AspI
AviII AvrII BalI BglI BlnI
BlpI Bpu1102I BsiWI BsmBI BspDI
Bst1107I BstZ17I CelII ClaI CpoI
DraIII Eam1105I Eco47III EcoRI FseI
FspI KpnI MluI MluNI MscI
NotI NruI PacI PflFI PinAI
PmeI PshAI PvuI RsrII SalI
ScaI SfiI SgrAI SnaBI SpeI
StuI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccIII 155 TCCGGA T^CCGGA
AclI 7747 AACGTT AA^CGTT
AcyI 61 GGCGCC G[GA]^CG[CT]C
AflII 3584 CTTAAG C^TTAAG
AocI 1119 CCTAAGG CC^T[ACGT]AGG
ApaI 4601 GGGCCC GGGCC^C
BamHI 2012 GGATCC G^GATCC
BbeI 61 GGCGCC GGCGC^C
BbrPI 6247 CACGTG CAC^GTG
BbvCI 2181 GCTGAGG CCTCAGC(-5/-2)
BcgI 1667 CGAGCCAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1739 GGATAC GTATCC(6/5)
BfrI 3584 CTTAAG C^TTAAG
BsaHI 61 GGCGCC G[GA]^CG[CT]C
BseAI 155 TCCGGA T^CCGGA
BsiEI 72 CGGCCG CG[GA][CT]^CG
BspEI 155 TCCGGA T^CCGGA
BsrBI 5812 CCGCTC CCGCTC(-3/-3)
BstEII 5481 GGTGACC G^GT[ACGT]ACC
BstPI 5481 GGTGACC G^GT[ACGT]ACC
Bsu36I 1119 CCTAAGG CC^T[ACGT]AGG
DrdI 6175 GACTGTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 72 CGGCCG [CT]^GGCC[GA]
EagI 72 CGGCCG C^GGCCG
EclXI 72 CGGCCG C^GGCCG
Eco52I 72 CGGCCG C^GGCCG
Eco81I 1119 CCTAAGG CC^T[ACGT]AGG
EcoNI 6678 CCTTCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 5481 GGTGACC G^GT[ACGT]ACC
EcoRV 5074 GATATC GAT^ATC
HgaI 5794 GCGTC GACGC(5/10)
Hin1I 61 GGCGCC G[GA]^CG[CT]C
HindIII 7073 AAGCTT A^AGCTT
HpaI 7017 GTTAAC GTT^AAC
KasI 61 GGCGCC G^GCGCC
KspI 4529 CCGCGG CCGC^GG
MroI 155 TCCGGA T^CCGGA
NarI 61 GGCGCC GG^CGCC
NcoI 2592 CCATGG C^CATGG
NdeI 7462 CATATG CA^TATG
PaeR7I 594 CTCGAG C^TCGAG
PflMI 5063 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 6247 CACGTG CAC^GTG
PmlI 6247 CACGTG CAC^GTG
Psp1406I 7747 AACGTT AA^CGTT
SacII 4529 CCGCGG CCGC^GG
SfoI 61 GGCGCC GGC^GCC
SmaI 5766 CCCGGG CCC^GGG
SphI 5975 GCATGC GCATG^C
Van91I 5063 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 567 CCAGTGCAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 594 CTCGAG C^TCGAG
XmaCI 5766 CCCGGG C^CCGGG
XmaI 5766 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Alw44I 5869 GTGCAC G^TGCAC
6640 GTGCAC
ApaLI 5869 GTGCAC G^TGCAC
6640 GTGCAC
AvaI 594 CTCGAG C^[CT]CG[GA]G
5766 CCCGGG
BanI 61 GGCGCC G^G[CT][GA]CC
2019 GGTGCC
BglII 758 AGATCT A^GATCT
3902 AGATCT
BsaWI 155 TCCGGA [AT]^CCGG[AT]
2247 TCCGGT
BsoBI 594 CTCGAG C^[CT]CG[GA]G
5766 CCCGGG
BspLU11I 345 ACATGT A^CATGT
7770 ACATGT
BsrFI 70 GCCGGC [GA]^CCGG[CT]
5774 GCCGGC
BssSI 1411 CACGAG CACGAG(-5/-1)
1313 CTCGTG
BstBI 1420 TTCGAA TT^CGAA
6702 TTCGAA
Cfr10I 70 GCCGGC [GA]^CCGG[CT]
5774 GCCGGC
EcoT22I 247 ATGCAT ATGCA^T
7548 ATGCAT
MfeI 1243 CAATTG C^AATTG
1672 CAATTG
MunI 1243 CAATTG C^AATTG
1672 CAATTG
NaeI 70 GCCGGC GCC^GGC
5774 GCCGGC
NgoMIV 70 GCCGGC G^CCGGC
5774 GCCGGC
NheI 1098 GCTAGC G^CTAGC
5582 GCTAGC
NsiI 247 ATGCAT ATGCA^T
7548 ATGCAT
NspV 1420 TTCGAA TT^CGAA
6702 TTCGAA
SbfI 674 CCTGCAGG CCTGCA^GG
2195 CCTGCAGG
SexAI 4311 ACCTGGT A^CC[AT]GGT
6218 ACCTGGT
SfuI 1420 TTCGAA TT^CGAA
6702 TTCGAA
Sse8387I 674 CCTGCAGG CCTGCA^GG
2195 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI AvaII BanII
BbsI BbvI BclI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaBI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspHI BspMI BsrDI
BsrGI BsrI BssHII BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PshBI PstI PvuII RcaI RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XhoII XmnI