##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AccIII AflII AgeI AhdI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BbeI BclI
BfrI BlnI BseAI BsiEI BsiWI
BsmI BspEI BspLU11I BssHII Bst1107I
BstZ17I CpoI DraIII DrdI EagI
Eam1105I EclXI Eco52I FbaI FseI
FspI HpaI KasI KpnI MfeI
MluI MroI MunI NarI NotI
NruI PacI PaeR7I PflFI PinAI
PmeI PshBI PvuI RsrII SbfI
ScaI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 1942 GACGTC GACGT^C
AclI 4296 AACGTT AA^CGTT
AflIII 778 ACGTGT A^C[GA][CT]GT
Alw44I 1905 GTGCAC G^TGCAC
Aor51HI 2842 AGCGCT AGC^GCT
ApaI 42 GGGCCC GGGCC^C
ApaLI 1905 GTGCAC G^TGCAC
Asp700I 1175 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 2806 CCCGGG C^[CT]CG[GA]G
BamHI 76 GGATCC G^GATCC
BbrPI 2045 CACGTG CAC^GTG
BbvCI 2553 GCTGAGG CCTCAGC(-5/-2)
BcgI 3386 GCAGCTGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 433 AGATCT A^GATCT
BsaBI 1884 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3322 GAGACC GGTCTC(1/5)
BsoBI 2806 CCCGGG C^[CT]CG[GA]G
BspDI 4 ATCGAT AT^CGAT
BsrBI 426 GAGCGG CCGCTC(-3/-3)
BstBI 844 TTCGAA TT^CGAA
ClaI 4 ATCGAT AT^CGAT
DraI 407 TTTAAA TTT^AAA
Eco47III 2842 AGCGCT AGC^GCT
EcoRV 4682 GATATC GAT^ATC
EcoT22I 2499 ATGCAT ATGCA^T
MamI 1884 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1810 GCCGGC GCC^GGC
NdeI 1149 CATATG CA^TATG
NgoMIV 1810 GCCGGC G^CCGGC
NsiI 2499 ATGCAT ATGCA^T
NspV 844 TTCGAA TT^CGAA
PmaCI 2045 CACGTG CAC^GTG
PmlI 2045 CACGTG CAC^GTG
PshAI 3507 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4296 AACGTT AA^CGTT
SalI 568 GTCGAC G^TCGAC
SexAI 1506 ACCTGGT A^CC[AT]GGT
SfuI 844 TTCGAA TT^CGAA
SmaI 2806 CCCGGG CCC^GGG
XbaI 3041 TCTAGA T^CTAGA
XmaCI 2806 CCCGGG C^CCGGG
XmaI 2806 CCCGGG C^CCGGG
XmnI 1175 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
BalI 2419 TGGCCA TGG^CCA
2572 TGGCCA
BciVI 5128 GGATAC GTATCC(6/5)
3379 GTATCC
BsaAI 1416 TACGTG [CT]AC^GT[GA]
2045 CACGTG
BsaWI 143 ACCGGA [AT]^CCGG[AT]
2149 TCCGGT
BsmBI 19 GAGACG CGTCTC(1/5)
1875 GAGACG
Bsp1407I 1145 TGTACA T^GTACA
4966 TGTACA
BsrFI 1810 GCCGGC [GA]^CCGG[CT]
3247 GCCGGT
BsrGI 1145 TGTACA T^GTACA
4966 TGTACA
BstEII 3024 GGTGACC G^GT[ACGT]ACC
4121 GGTGACC
BstPI 3024 GGTGACC G^GT[ACGT]ACC
4121 GGTGACC
BstXI 667 CCAAAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3415 CCAGTCCACTGG
Cfr10I 1810 GCCGGC [GA]^CCGG[CT]
3247 GCCGGT
EcoO65I 3024 GGTGACC G^GT[ACGT]ACC
4121 GGTGACC
FauI 188 CCCGC CCCGC(4/6)
255 CCCGC
KspI 10 CCGCGG CCGC^GG
256 CCGCGG
MluNI 2419 TGGCCA TGG^CCA
2572 TGGCCA
MscI 2419 TGGCCA TGG^CCA
2572 TGGCCA
NcoI 1643 CCATGG C^CATGG
3527 CCATGG
NheI 3596 GCTAGC G^CTAGC
4771 GCTAGC
NspI 729 ACATGC [GA]CATG^[CT]
1138 ACATGC
PflMI 1321 CCAGTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3754 CCATTGGCTGG
SacII 10 CCGCGG CCGC^GG
256 CCGCGG
SspBI 1145 TGTACA T^GTACA
4966 TGTACA
SspI 3927 AATATT AAT^ATT
4933 AATATT
Van91I 1321 CCAGTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3754 CCATTGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApoI AspHI AvaII BanI BanII
BbsI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspHI BspMI BsrDI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RcaI RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII