##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccI AclI AgeI Aor51HI
AscI AseI AsnI AspI BbrPI
BclI BsaBI BsiEI BsiWI BspDI
BspHI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I EcoRV FbaI FseI
HincII HindII HindIII HpaI MamI
NheI NotI NruI NspV PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 2064 GGTACC G^GTACC
Asp718I 2064 GGTACC G^GTACC
AviII 711 TGCGCA TGC^GCA
AvrII 377 CCTAGG C^CTAGG
BcgI 6378 CGAGCCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5408 GTATCC GTATCC(6/5)
BglII 6726 AGATCT A^GATCT
BlnI 377 CCTAGG C^CTAGG
BsaAI 1593 TACGTG [CT]AC^GT[GA]
BsmBI 1669 CGTCTC CGTCTC(1/5)
BspLU11I 5416 ACATGT A^CATGT
BssSI 2137 CACGAG CACGAG(-5/-1)
DraIII 3686 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 3039 CCTGAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 6960 ATGCAT ATGCA^T
FspI 711 TGCGCA TGC^GCA
KpnI 2064 GGTACC GGTAC^C
KspI 1278 CCGCGG CCGC^GG
MfeI 354 CAATTG C^AATTG
MluI 4 ACGCGT A^CGCGT
MunI 354 CAATTG C^AATTG
NdeI 4428 CATATG CA^TATG
NsiI 6960 ATGCAT ATGCA^T
PacI 6340 TTAATTAA TTAAT^TAA
SacII 1278 CCGCGG CCGC^GG
SexAI 6390 ACCTGGT A^CC[AT]GGT
SfiI 1084 GGCCTGCTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 3776 AGGCCT AGG^CCT
XbaI 3584 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 265 TCCGGA T^CCGGA
2564 TCCGGA
AcyI 1350 GGCGCC G[GA]^CG[CT]C
3144 GGCGCC
AhdI 1493 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3705 GACCAGCAGTC
AspEI 1493 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3705 GACCAGCAGTC
BbeI 1350 GGCGCC GGCGC^C
3144 GGCGCC
BlpI 3917 GCTCAGC GC^T[ACGT]AGC
5075 GCTGAGC
Bpu1102I 3917 GCTCAGC GC^T[ACGT]AGC
5075 GCTGAGC
BsaHI 1350 GGCGCC G[GA]^CG[CT]C
3144 GGCGCC
BsaI 2777 GAGACC GGTCTC(1/5)
1837 GGTCTC
BseAI 265 TCCGGA T^CCGGA
2564 TCCGGA
BspEI 265 TCCGGA T^CCGGA
2564 TCCGGA
BsrBI 1973 CCGCTC CCGCTC(-3/-3)
43 GAGCGG
BstEII 1282 GGTCACC G^GT[ACGT]ACC
3804 GGTGACC
BstPI 1282 GGTCACC G^GT[ACGT]ACC
3804 GGTGACC
CelII 3917 GCTCAGC GC^T[ACGT]AGC
5075 GCTGAGC
Eam1105I 1493 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3705 GACCAGCAGTC
EcoO65I 1282 GGTCACC G^GT[ACGT]ACC
3804 GGTGACC
EcoRI 6139 GAATTC G^AATTC
6734 GAATTC
HgaI 3 GACGC GACGC(5/10)
4500 GCGTC
Hin1I 1350 GGCGCC G[GA]^CG[CT]C
3144 GGCGCC
KasI 1350 GGCGCC G^GCGCC
3144 GGCGCC
MroI 265 TCCGGA T^CCGGA
2564 TCCGGA
NaeI 23 GCCGGC GCC^GGC
7124 GCCGGC
NarI 1350 GGCGCC GG^CGCC
3144 GGCGCC
NcoI 942 CCATGG C^CATGG
4867 CCATGG
NgoMIV 23 GCCGGC G^CCGGC
7124 GCCGGC
PshAI 2511 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2568 GACTCTCGTC
SfoI 1350 GGCGCC GGC^GCC
3144 GGCGCC
TaqI 442 TCGA T^CGA
3507 TCGA
TthHB8I 442 TCGA T^CGA
3507 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI Asp700I
AspHI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BfrI BglI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspMI BsrDI BsrFI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SphI SspBI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XcmI XhoII XmaCI
XmaI XmnI