##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
AatII AgeI Aor51HI AscI AviII
AvrII BbvCI BlnI BsaBI BsiWI
BsmBI BsrBI BssSI BstBI CpoI
Eco47III EcoRI EcoRV FseI FspI
HpaI MamI MfeI MluI MunI
NheI NotI NruI NspV PacI
PflMI PinAI PmeI RsrII SalI
SbfI ScaI SexAI SfuI SgrAI
SmaI Sse8387I Van91I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 671 GGTACC G^GTACC
AhdI 4516 GACTGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 6347 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2271 CCTCAGG CC^T[ACGT]AGG
ApaI 5341 GGGCCC GGGCC^C
AseI 3777 ATTAAT AT^TAAT
AsnI 3777 ATTAAT AT^TAAT
Asp718I 671 GGTACC G^GTACC
AspEI 4516 GACTGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5742 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BlpI 4028 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4028 GCTCAGC GC^T[ACGT]AGC
Bsp1407I 6043 TGTACA T^GTACA
BspDI 2011 ATCGAT AT^CGAT
BsrGI 6043 TGTACA T^GTACA
BssHII 3201 GCGCGC G^CGCGC
Bst1107I 5795 GTATAC GTA^TAC
BstAPI 1431 GCAAGGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 5795 GTATAC GTA^TAC
Bsu36I 2271 CCTCAGG CC^T[ACGT]AGG
CelII 4028 GCTCAGC GC^T[ACGT]AGC
ClaI 2011 ATCGAT AT^CGAT
DraIII 3991 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 6411 GACATTCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1505 CGGCCG C^GGCCG
Eam1105I 4516 GACTGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1505 CGGCCG C^GGCCG
Eco52I 1505 CGGCCG C^GGCCG
Eco81I 2271 CCTCAGG CC^T[ACGT]AGG
HindIII 3381 AAGCTT A^AGCTT
KpnI 671 GGTACC GGTAC^C
KspI 2757 CCGCGG CCGC^GG
NaeI 289 GCCGGC GCC^GGC
NdeI 191 CATATG CA^TATG
NgoMIV 289 GCCGGC G^CCGGC
PaeR7I 902 CTCGAG C^TCGAG
PflFI 5742 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3777 ATTAAT AT^TAAT
PvuI 725 CGATCG CGAT^CG
SacII 2757 CCGCGG CCGC^GG
SnaBI 5813 TACGTA TAC^GTA
SphI 1520 GCATGC GCATG^C
SspBI 6043 TGTACA T^GTACA
SwaI 1904 ATTTAAAT ATTT^AAAT
Tth111I 5742 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 902 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 1821 TCCGGA T^CCGGA
2117 TCCGGA
AclI 843 AACGTT AA^CGTT
6818 AACGTT
AflII 3650 CTTAAG C^TTAAG
3762 CTTAAG
AvaI 902 CTCGAG C^[CT]CG[GA]G
6306 CCCGAG
BamHI 1007 GGATCC G^GATCC
1824 GGATCC
BbeI 47 GGCGCC GGCGC^C
499 GGCGCC
BciVI 999 GTATCC GTATCC(6/5)
1339 GTATCC
BclI 1865 TGATCA T^GATCA
5497 TGATCA
BfrI 3650 CTTAAG C^TTAAG
3762 CTTAAG
BglI 1064 GCCTCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3016 GCCACCCTGGC
BglII 2899 AGATCT A^GATCT
3692 AGATCT
BseAI 1821 TCCGGA T^CCGGA
2117 TCCGGA
BsiEI 725 CGATCG CG[GA][CT]^CG
1505 CGGCCG
BsmI 3999 GCATTC GAATGC(1/-1)
6151 GCATTC
BsoBI 902 CTCGAG C^[CT]CG[GA]G
6306 CCCGAG
BspEI 1821 TCCGGA T^CCGGA
2117 TCCGGA
BstEII 857 GGTTACC G^GT[ACGT]ACC
5229 GGTCACC
BstPI 857 GGTTACC G^GT[ACGT]ACC
5229 GGTCACC
EcoNI 864 CCTGTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3851 CCTGAGATAGG
EcoO65I 857 GGTTACC G^GT[ACGT]ACC
5229 GGTCACC
EcoT22I 4241 ATGCAT ATGCA^T
5646 ATGCAT
FbaI 1865 TGATCA T^GATCA
5497 TGATCA
HincII 2862 GTTGAC GT[CT]^[GA]AC
6436 GTTGAC
HindII 2862 GTTGAC GT[CT]^[GA]AC
6436 GTTGAC
KasI 47 GGCGCC G^GCGCC
499 GGCGCC
MroI 1821 TCCGGA T^CCGGA
2117 TCCGGA
NarI 47 GGCGCC GG^CGCC
499 GGCGCC
NsiI 4241 ATGCAT ATGCA^T
5646 ATGCAT
PpuMI 2971 AGGACCC [GA]G^G[AT]CC[CT]
5550 GGGTCCT
Psp1406I 843 AACGTT AA^CGTT
6818 AACGTT
SacI 538 GAGCTC GAGCT^C
582 GAGCTC
SapI 284 GAAGAGC GCTCTTC(1/4)
2277 GAAGAGC
SfiI 1063 GGCCTCCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3015 GGCCACCCTGGCC
SfoI 47 GGCGCC GGC^GCC
499 GGCGCC
SpeI 2785 ACTAGT A^CTAGT
3069 ACTAGT
XcmI 5318 CCATATTCACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5986 CCAGAACCCATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
ApaLI ApoI Asp700I AspHI AvaII
BalI BanI BanII BbrPI BbsI
BbvI BcgI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspHI BspLU11I BspMI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PmaCI
PmlI PshAI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI