################################## # NO CUT ENZYMES ( 51 enzymes) # ################################## Acc65I AclI AgeI Alw44I ApaLI AscI AseI AsnI Asp718I BbeI BciVI BsaBI BsiWI BspHI BssHII BstBI CpoI EcoRV EcoT22I FseI HindIII HpaI KasI KpnI KspI MamI MfeI MluI MunI NarI NheI NotI NruI NsiI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SfoI SfuI SgrAI SnaBI SpeI SwaI XbaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 1557 GACGTC GACGT^C AccIII 337 TCCGGA T^CCGGA AcyI 1557 GACGTC G[GA]^CG[CT]C AflII 639 CTTAAG C^TTAAG AhdI 3965 GACAACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 4059 AGCGCT AGC^GCT AspEI 3965 GACAACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3343 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 2736 CCTAGG C^CTAGG BbrPI 2813 CACGTG CAC^GTG BfrI 639 CTTAAG C^TTAAG BlnI 2736 CCTAGG C^CTAGG BsaHI 1557 GACGTC G[GA]^CG[CT]C BseAI 337 TCCGGA T^CCGGA BsmI 1125 GAATGC GAATGC(1/-1) Bsp1407I 6222 TGTACA T^GTACA BspDI 1752 ATCGAT AT^CGAT BspEI 337 TCCGGA T^CCGGA BspLU11I 4023 ACATGT A^CATGT BsrBI 1422 GAGCGG CCGCTC(-3/-3) BsrGI 6222 TGTACA T^GTACA Bst1107I 5651 GTATAC GTA^TAC BstZ17I 5651 GTATAC GTA^TAC ClaI 1752 ATCGAT AT^CGAT Eam1105I 3965 GACAACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 4059 AGCGCT AGC^GCT HaeII 4059 AGCGCT [GA]GCGC^[CT] Hin1I 1557 GACGTC G[GA]^CG[CT]C HincII 1449 GTCGAC GT[CT]^[GA]AC HindII 1449 GTCGAC GT[CT]^[GA]AC MroI 337 TCCGGA T^CCGGA NcoI 417 CCATGG C^CATGG NdeI 5786 CATATG CA^TATG PaeR7I 2652 CTCGAG C^TCGAG PflFI 3343 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2813 CACGTG CAC^GTG PmlI 2813 CACGTG CAC^GTG PshAI 786 GACGTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SalI 1449 GTCGAC G^TCGAC SbfI 4647 CCTGCAGG CCTGCA^GG ScaI 1465 AGTACT AGT^ACT SexAI 1937 ACCTGGT A^CC[AT]GGT Sse8387I 4647 CCTGCAGG CCTGCA^GG SspBI 6222 TGTACA T^GTACA Tth111I 3343 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2652 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### BbsI 2057 GAAGAC GAAGAC(2/6) 828 GTCTTC BclI 349 TGATCA T^GATCA 3140 TGATCA BglII 1072 AGATCT A^GATCT 5262 AGATCT BpuAI 2057 GAAGAC GAAGAC(2/6) 828 GTCTTC BsaAI 1285 CACGTA [CT]AC^GT[GA] 2813 CACGTG BsaWI 337 TCCGGA [AT]^CCGG[AT] 5180 TCCGGT BsiEI 2766 CGGCCG CG[GA][CT]^CG 3426 CGGCCG BsmBI 5619 CGTCTC CGTCTC(1/5) 738 GAGACG BssSI 5622 CTCGTG CACGAG(-5/-1) 6422 CTCGTG BstXI 2258 CCAAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5850 CCATGCCCTTGG DrdI 2247 GACTCAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4394 GACAAATGTGTC EagI 2766 CGGCCG C^GGCCG 3426 CGGCCG EclXI 2766 CGGCCG C^GGCCG 3426 CGGCCG Eco52I 2766 CGGCCG C^GGCCG 3426 CGGCCG EcoRI 2703 GAATTC G^AATTC 3792 GAATTC FbaI 349 TGATCA T^GATCA 3140 TGATCA HgaI 740 GACGC GACGC(5/10) 1165 GCGTC NaeI 1406 GCCGGC GCC^GGC 2296 GCCGGC NgoMIV 1406 GCCGGC G^CCGGC 2296 GCCGGC SapI 4049 GAAGAGC GCTCTTC(1/4) 1580 GCTCTTC SfiI 5860 GGCCTCGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 5869 GGCCACATTGGCC SmaI 2500 CCCGGG CCC^GGG 3564 CCCGGG XcmI 1086 CCAGCCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5243 CCAGGTCAGAGGTGG XmaCI 2500 CCCGGG C^CCGGG 3564 CCCGGG XmaI 2500 CCCGGG C^CCGGG 3564 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 136 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApaI ApoI Asp700I AspHI AvaI AvaII AviII BalI BamHI BanI BanII BbvCI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI