##################################
# NO CUT ENZYMES (   51 enzymes) #
##################################

Acc65I      AclI        AgeI        Alw44I      ApaLI       
AscI        AseI        AsnI        Asp718I     BbeI        
BciVI       BsaBI       BsiWI       BspHI       BssHII      
BstBI       CpoI        EcoRV       EcoT22I     FseI        
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MunI        NarI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PinAI       PmeI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SfoI        
SfuI        SgrAI       SnaBI       SpeI        SwaI        
XbaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII        1557  GACGTC               GACGT^C
AccIII        337  TCCGGA               T^CCGGA
AcyI         1557  GACGTC               G[GA]^CG[CT]C
AflII         639  CTTAAG               C^TTAAG
AhdI         3965  GACAACTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      4059  AGCGCT               AGC^GCT
AspEI        3965  GACAACTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         3343  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        2736  CCTAGG               C^CTAGG
BbrPI        2813  CACGTG               CAC^GTG
BfrI          639  CTTAAG               C^TTAAG
BlnI         2736  CCTAGG               C^CTAGG
BsaHI        1557  GACGTC               G[GA]^CG[CT]C
BseAI         337  TCCGGA               T^CCGGA
BsmI         1125  GAATGC               GAATGC(1/-1)
Bsp1407I     6222  TGTACA               T^GTACA
BspDI        1752  ATCGAT               AT^CGAT
BspEI         337  TCCGGA               T^CCGGA
BspLU11I     4023  ACATGT               A^CATGT
BsrBI        1422  GAGCGG               CCGCTC(-3/-3)
BsrGI        6222  TGTACA               T^GTACA
Bst1107I     5651  GTATAC               GTA^TAC
BstZ17I      5651  GTATAC               GTA^TAC
ClaI         1752  ATCGAT               AT^CGAT
Eam1105I     3965  GACAACTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     4059  AGCGCT               AGC^GCT
HaeII        4059  AGCGCT               [GA]GCGC^[CT]
Hin1I        1557  GACGTC               G[GA]^CG[CT]C
HincII       1449  GTCGAC               GT[CT]^[GA]AC
HindII       1449  GTCGAC               GT[CT]^[GA]AC
MroI          337  TCCGGA               T^CCGGA
NcoI          417  CCATGG               C^CATGG
NdeI         5786  CATATG               CA^TATG
PaeR7I       2652  CTCGAG               C^TCGAG
PflFI        3343  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        2813  CACGTG               CAC^GTG
PmlI         2813  CACGTG               CAC^GTG
PshAI         786  GACGTGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI         1449  GTCGAC               G^TCGAC
SbfI         4647  CCTGCAGG             CCTGCA^GG
ScaI         1465  AGTACT               AGT^ACT
SexAI        1937  ACCTGGT              A^CC[AT]GGT
Sse8387I     4647  CCTGCAGG             CCTGCA^GG
SspBI        6222  TGTACA               T^GTACA
Tth111I      3343  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         2652  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

BbsI         2057  GAAGAC               GAAGAC(2/6)
              828  GTCTTC              
BclI          349  TGATCA               T^GATCA
             3140  TGATCA              
BglII        1072  AGATCT               A^GATCT
             5262  AGATCT              
BpuAI        2057  GAAGAC               GAAGAC(2/6)
              828  GTCTTC              
BsaAI        1285  CACGTA               [CT]AC^GT[GA]
             2813  CACGTG              
BsaWI         337  TCCGGA               [AT]^CCGG[AT]
             5180  TCCGGT              
BsiEI        2766  CGGCCG               CG[GA][CT]^CG
             3426  CGGCCG              
BsmBI        5619  CGTCTC               CGTCTC(1/5)
              738  GAGACG              
BssSI        5622  CTCGTG               CACGAG(-5/-1)
             6422  CTCGTG              
BstXI        2258  CCAAGTGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5850  CCATGCCCTTGG        
DrdI         2247  GACTCAGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4394  GACAAATGTGTC        
EagI         2766  CGGCCG               C^GGCCG
             3426  CGGCCG              
EclXI        2766  CGGCCG               C^GGCCG
             3426  CGGCCG              
Eco52I       2766  CGGCCG               C^GGCCG
             3426  CGGCCG              
EcoRI        2703  GAATTC               G^AATTC
             3792  GAATTC              
FbaI          349  TGATCA               T^GATCA
             3140  TGATCA              
HgaI          740  GACGC                GACGC(5/10)
             1165  GCGTC               
NaeI         1406  GCCGGC               GCC^GGC
             2296  GCCGGC              
NgoMIV       1406  GCCGGC               G^CCGGC
             2296  GCCGGC              
SapI         4049  GAAGAGC              GCTCTTC(1/4)
             1580  GCTCTTC             
SfiI         5860  GGCCTCGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             5869  GGCCACATTGGCC       
SmaI         2500  CCCGGG               CCC^GGG
             3564  CCCGGG              
XcmI         1086  CCAGCCAGCCGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             5243  CCAGGTCAGAGGTGG     
XmaCI        2500  CCCGGG               C^CCGGG
             3564  CCCGGG              
XmaI         2500  CCCGGG               C^CCGGG
             3564  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  136 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AflIII      AluI        AlwI        AlwNI       AocI        
ApaI        ApoI        Asp700I     AspHI       AvaI        
AvaII       AviII       BalI        BamHI       BanI        
BanII       BbvCI       BbvI        BcgI        BcnI        
BfaI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrDI       BsrFI       
BsrI        BssKI       BstAPI      BstEII      BstF5I      
BstNI       BstPI       BstUI       BstYI       Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraI        DraII       DraIII      
DsaI        EaeI        EarI        Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        FspI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SphI        SspI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII       
XmnI