##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI Aor51HI AscI Asp718I AviII
BcgI BfrI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BspHI BsrBI
BsrFI BssHII BstBI BstEII BstPI
BstUI Cfr10I ClaI CpoI EagI
EclXI Eco47III Eco52I EcoO65I FseI
FspI KpnI KspI MluI MroI
MvnI NaeI NgoMIV NheI NotI
NruI NspV PacI PinAI PmeI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfuI SgrAI SnaBI Sse8387I StuI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 1261 GACGTC GACGT^C
Alw44I 440 GTGCAC G^TGCAC
ApaLI 440 GTGCAC G^TGCAC
AspI 874 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4331 CCTAGG C^CTAGG
BbeI 4373 GGCGCC GGCGC^C
BbrPI 1240 CACGTG CAC^GTG
BglII 336 AGATCT A^GATCT
BlnI 4331 CCTAGG C^CTAGG
BsmI 5491 GCATTC GAATGC(1/-1)
BsrDI 1034 CATTGC GCAATG(2/0)
Bst1107I 4462 GTATAC GTA^TAC
BstZ17I 4462 GTATAC GTA^TAC
DraIII 3343 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1488 GACAGCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 645 CCATGG C^C[GA][CT]GG
EcoNI 4259 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 4373 GGCGCC [GA]GCGC^[CT]
HincII 3462 GTTAAC GT[CT]^[GA]AC
HindII 3462 GTTAAC GT[CT]^[GA]AC
HpaI 3462 GTTAAC GTT^AAC
KasI 4373 GGCGCC G^GCGCC
NarI 4373 GGCGCC GG^CGCC
NcoI 645 CCATGG C^CATGG
PaeR7I 332 CTCGAG C^TCGAG
PflFI 874 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4202 CCAAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1240 CACGTG CAC^GTG
PmlI 1240 CACGTG CAC^GTG
PpuMI 812 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 2978 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3672 GAGCTC GAGCT^C
SfoI 4373 GGCGCC GGC^GCC
SmaI 2519 CCCGGG CCC^GGG
SwaI 5746 ATTTAAAT ATTT^AAAT
Tth111I 874 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4202 CCAAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 332 CTCGAG C^TCGAG
XmaCI 2519 CCCGGG C^CCGGG
XmaI 2519 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1324 GTCTAC GT^[AC][GT]AC
4462 GTATAC
AflIII 5996 ACATGT A^C[GA][CT]GT
6183 ACATGT
AocI 1212 CCTAAGG CC^T[ACGT]AGG
3467 CCTCAGG
Asp700I 3556 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4805 GAATAATTTC
BciVI 3111 GGATAC GTATCC(6/5)
3175 GGATAC
BlpI 3087 GCTCAGC GC^T[ACGT]AGC
5223 GCTAAGC
Bpu1102I 3087 GCTCAGC GC^T[ACGT]AGC
5223 GCTAAGC
BsaAI 1240 CACGTG [CT]AC^GT[GA]
5817 CACGTA
BsaBI 724 GATACCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1158 GATCCAGATC
BsaI 487 GGTCTC GGTCTC(1/5)
2454 GGTCTC
BspLU11I 5996 ACATGT A^CATGT
6183 ACATGT
BssSI 3071 CTCGTG CACGAG(-5/-1)
5342 CTCGTG
BstAPI 583 GCATCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5412 GCATAATGTGC
Bsu36I 1212 CCTAAGG CC^T[ACGT]AGG
3467 CCTCAGG
CelII 3087 GCTCAGC GC^T[ACGT]AGC
5223 GCTAAGC
Eco81I 1212 CCTAAGG CC^T[ACGT]AGG
3467 CCTCAGG
EcoRV 115 GATATC GAT^ATC
1010 GATATC
EcoT22I 209 ATGCAT ATGCA^T
3027 ATGCAT
HgaI 1575 GCGTC GACGC(5/10)
5182 GCGTC
HindIII 2799 AAGCTT A^AGCTT
5916 AAGCTT
MamI 724 GATACCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1158 GATCCAGATC
MfeI 1775 CAATTG C^AATTG
3626 CAATTG
MunI 1775 CAATTG C^AATTG
3626 CAATTG
NsiI 209 ATGCAT ATGCA^T
3027 ATGCAT
PstI 1205 CTGCAG CTGCA^G
2968 CTGCAG
ScaI 3138 AGTACT AGT^ACT
3522 AGTACT
SpeI 5602 ACTAGT A^CTAGT
6237 ACTAGT
SphI 983 GCATGC GCATG^C
5285 GCATGC
XbaI 1920 TCTAGA T^CTAGA
2869 TCTAGA
XmnI 3556 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4805 GAATAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AciI AcsI AcyI AfaI AhdI
AluI AlwI AlwNI ApaI ApoI
AseI AsnI AspEI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbsI BbvCI BbvI BclI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrGI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EaeI Eam1105I
EarI EcoO109I EcoRI EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeI NdeII NlaIII NlaIV NspI
PleI PshBI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII