##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccIII Alw44I ApaLI AscI
AviII BbeI BclI BsaBI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BssHII BssSI Bst1107I BstBI BstZ17I
ClaI CpoI DraIII DrdI EagI
EclXI Eco52I EcoRV FauI FbaI
FseI FspI KasI KspI MamI
MluI MroI NaeI NarI NgoMIV
NotI NruI NspV PacI PaeR7I
PmeI PvuI RcaI RsrII SacI
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI TaqI TthHB8I XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 4721 GTCTAC GT^[AC][GT]AC
AccII 122 CGCG CG^CG
AclI 5501 AACGTT AA^CGTT
AcyI 931 GACGCC G[GA]^CG[CT]C
AflII 4991 CTTAAG C^TTAAG
AgeI 673 ACCGGT A^CCGGT
AocI 3369 CCTTAGG CC^T[ACGT]AGG
Aor51HI 864 AGCGCT AGC^GCT
BbsI 3125 GAAGAC GAAGAC(2/6)
BbvCI 199 CCTCAGC CCTCAGC(-5/-2)
BciVI 3915 GGATAC GTATCC(6/5)
BfrI 4991 CTTAAG C^TTAAG
BglII 392 AGATCT A^GATCT
BpuAI 3125 GAAGAC GAAGAC(2/6)
BsaHI 931 GACGCC G[GA]^CG[CT]C
BsmBI 760 CGTCTC CGTCTC(1/5)
BsrBI 5039 CCGCTC CCGCTC(-3/-3)
BsrFI 673 ACCGGT [GA]^CCGG[CT]
BstAPI 3705 GCAAAATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3004 GGTCACC G^GT[ACGT]ACC
BstPI 3004 GGTCACC G^GT[ACGT]ACC
BstUI 122 CGCG CG^CG
BstXI 1191 CCAGACGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3369 CCTTAGG CC^T[ACGT]AGG
Cfr10I 673 ACCGGT [GA]^CCGG[CT]
Eco47III 864 AGCGCT AGC^GCT
Eco81I 3369 CCTTAGG CC^T[ACGT]AGG
EcoO65I 3004 GGTCACC G^GT[ACGT]ACC
HaeII 864 AGCGCT [GA]GCGC^[CT]
Hin1I 931 GACGCC G[GA]^CG[CT]C
MvnI 122 CGCG CG^CG
NheI 3341 GCTAGC G^CTAGC
PflMI 5763 CCAGGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 673 ACCGGT A^CCGGT
PshAI 3645 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 5501 AACGTT AA^CGTT
Van91I 5763 CCAGGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 606 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 4497 GGTACC G^GTACC
5308 GGTACC
ApaI 1879 GGGCCC GGGCC^C
2821 GGGCCC
AseI 1606 ATTAAT AT^TAAT
3993 ATTAAT
AsnI 1606 ATTAAT AT^TAAT
3993 ATTAAT
Asp718I 4497 GGTACC G^GTACC
5308 GGTACC
AspI 3311 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4846 GACAGAGTC
BamHI 3933 GGATCC G^GATCC
3961 GGATCC
BbrPI 827 CACGTG CAC^GTG
4628 CACGTG
BsaI 2685 GAGACC GGTCTC(1/5)
648 GGTCTC
BsaWI 673 ACCGGT [AT]^CCGG[AT]
1317 ACCGGA
BsmI 2120 GAATGC GAATGC(1/-1)
5442 GAATGC
Bsp1407I 3285 TGTACA T^GTACA
4341 TGTACA
BspLU11I 5122 ACATGT A^CATGT
5648 ACATGT
BsrGI 3285 TGTACA T^GTACA
4341 TGTACA
CfoI 865 GCGC GCG^C
4684 GCGC
EcoNI 4477 CCTAAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5853 CCTCACTCAGG
EcoRI 2782 GAATTC G^AATTC
6021 GAATTC
EcoT22I 1259 ATGCAT ATGCA^T
3522 ATGCAT
HgaI 931 GACGC GACGC(5/10)
1095 GACGC
HhaI 865 GCGC GCG^C
4684 GCGC
HinP1I 865 GCGC G^CGC
4684 GCGC
HindIII 3978 AAGCTT A^AGCTT
5383 AAGCTT
HpaI 5223 GTTAAC GTT^AAC
5689 GTTAAC
KpnI 4497 GGTACC GGTAC^C
5308 GGTACC
NcoI 1385 CCATGG C^CATGG
3357 CCATGG
NsiI 1259 ATGCAT ATGCA^T
3522 ATGCAT
PflFI 3311 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4846 GACAGAGTC
PmaCI 827 CACGTG CAC^GTG
4628 CACGTG
PmlI 827 CACGTG CAC^GTG
4628 CACGTG
PshBI 1606 ATTAAT AT^TAAT
3993 ATTAAT
ScaI 75 AGTACT AGT^ACT
3843 AGTACT
SexAI 2213 ACCAGGT A^CC[AT]GGT
2899 ACCTGGT
SmaI 1301 CCCGGG CCC^GGG
5895 CCCGGG
SphI 2345 GCATGC GCATG^C
4291 GCATGC
SspBI 3285 TGTACA T^GTACA
4341 TGTACA
Tth111I 3311 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4846 GACAGAGTC
XmaCI 1301 CCCGGG C^CCGGG
5895 CCCGGG
XmaI 1301 CCCGGG C^CCGGG
5895 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AfaI AflIII AhdI
AluI AlwI AlwNI ApoI Asp700I
AspEI AspHI AvaI AvaII AvrII
BalI BanI BanII BbvI BcgI
BcnI BfaI BglI BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CelII Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
Eam1105I EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MunI MvaI MwoI NciI NdeI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII XmnI