##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AclI AcsI AhdI Aor51HI ApoI
AscI AseI AsnI Asp700I AspEI
BsaBI BsiWI BspDI BspHI Bst1107I
BstZ17I ClaI DraI Eam1105I Eco47III
EcoRI EcoRV HincII HindII HpaI
MamI MluI NotI NruI PacI
PaeR7I PmeI PshAI PshBI Psp1406I
PvuI RcaI SalI SbfI ScaI
SgrAI SpeI Sse8387I SspI SwaI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 5807 GACGTC GACGT^C
Acc65I 1783 GGTACC G^GTACC
AflII 3081 CTTAAG C^TTAAG
Asp718I 1783 GGTACC G^GTACC
AviII 4967 TGCGCA TGC^GCA
AvrII 4320 CCTAGG C^CTAGG
BbeI 7466 GGCGCC GGCGC^C
BclI 635 TGATCA T^GATCA
BfrI 3081 CTTAAG C^TTAAG
BlnI 4320 CCTAGG C^CTAGG
BsmBI 1268 CGTCTC CGTCTC(1/5)
Bsp1407I 6324 TGTACA T^GTACA
BsrGI 6324 TGTACA T^GTACA
BssHII 5118 GCGCGC G^CGCGC
BstBI 5506 TTCGAA TT^CGAA
BstEII 1058 GGTGACC G^GT[ACGT]ACC
BstPI 1058 GGTGACC G^GT[ACGT]ACC
CpoI 2662 CGGACCG CG^G[AT]CCG
EcoO65I 1058 GGTGACC G^GT[ACGT]ACC
FbaI 635 TGATCA T^GATCA
FseI 5297 GGCCGGCC GGCCGG^CC
FspI 4967 TGCGCA TGC^GCA
HindIII 5965 AAGCTT A^AGCTT
KasI 7466 GGCGCC G^GCGCC
KpnI 1783 GGTACC GGTAC^C
NarI 7466 GGCGCC GG^CGCC
NdeI 4717 CATATG CA^TATG
NheI 5429 GCTAGC G^CTAGC
NspV 5506 TTCGAA TT^CGAA
RsrII 2662 CGGACCG CG^G[AT]CCG
SapI 3935 GAAGAGC GCTCTTC(1/4)
SfiI 7340 GGCCCCTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 7466 GGCGCC GGC^GCC
SfuI 5506 TTCGAA TT^CGAA
SnaBI 6555 TACGTA TAC^GTA
SspBI 6324 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 4663 TCCGGA T^CCGGA
5384 TCCGGA
AgeI 520 ACCGGT A^CCGGT
1039 ACCGGT
AspI 29 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5172 GACCGTGTC
BciVI 615 GGATAC GTATCC(6/5)
4392 GGATAC
BglII 4922 AGATCT A^GATCT
5272 AGATCT
BlpI 490 GCTCAGC GC^T[ACGT]AGC
3158 GCTGAGC
Bpu1102I 490 GCTCAGC GC^T[ACGT]AGC
3158 GCTGAGC
BseAI 4663 TCCGGA T^CCGGA
5384 TCCGGA
BspEI 4663 TCCGGA T^CCGGA
5384 TCCGGA
BspLU11I 3987 ACATGT A^CATGT
4755 ACATGT
CelII 490 GCTCAGC GC^T[ACGT]AGC
3158 GCTGAGC
DrdI 3111 GACCGCCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6568 GACTGCAGTGTC
EagI 1727 CGGCCG C^GGCCG
4360 CGGCCG
EclXI 1727 CGGCCG C^GGCCG
4360 CGGCCG
Eco52I 1727 CGGCCG C^GGCCG
4360 CGGCCG
EcoT22I 1297 ATGCAT ATGCA^T
5514 ATGCAT
KspI 485 CCGCGG CCGC^GG
6479 CCGCGG
MfeI 2291 CAATTG C^AATTG
5903 CAATTG
MroI 4663 TCCGGA T^CCGGA
5384 TCCGGA
MunI 2291 CAATTG C^AATTG
5903 CAATTG
NsiI 1297 ATGCAT ATGCA^T
5514 ATGCAT
PflFI 29 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5172 GACCGTGTC
PinAI 520 ACCGGT A^CCGGT
1039 ACCGGT
SacII 485 CCGCGG CCGC^GG
6479 CCGCGG
SexAI 1858 ACCAGGT A^CC[AT]GGT
3208 ACCTGGT
Tth111I 29 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5172 GACCGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbrPI BbsI BbvCI BbvI BcgI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SphI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI