##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI Aor51HI AscI AseI AsnI
Asp718I AviII AvrII BbeI BbvCI
BclI BlnI BsaBI BsaHI BseAI
BsiWI BspDI BspEI BspHI BsrBI
BstEII BstPI ClaI CpoI DrdI
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NarI NheI NotI NruI NsiI
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SfoI SgrAI SnaBI SpeI Sse8387I
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 4453 GTATAC GT^[AC][GT]AC
AflII 4356 CTTAAG C^TTAAG
AhdI 4098 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2467 GTGCAC G^TGCAC
ApaLI 2467 GTGCAC G^TGCAC
Asp700I 706 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4098 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2289 GGATCC G^GATCC
BanI 4778 GGTGCC G^G[CT][GA]CC
BbrPI 2308 CACGTG CAC^GTG
BfrI 4356 CTTAAG C^TTAAG
BsaWI 403 ACCGGA [AT]^CCGG[AT]
BspLU11I 501 ACATGT A^CATGT
BsrDI 5227 CATTGC GCAATG(2/0)
BssSI 671 CTCGTG CACGAG(-5/-1)
Bst1107I 4453 GTATAC GTA^TAC
BstBI 2707 TTCGAA TT^CGAA
BstZ17I 4453 GTATAC GTA^TAC
DraIII 827 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 202 CGGCCG C^GGCCG
Eam1105I 4098 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 202 CGGCCG C^GGCCG
Eco52I 202 CGGCCG C^GGCCG
HincII 1793 GTTGAC GT[CT]^[GA]AC
HindII 1793 GTTGAC GT[CT]^[GA]AC
NdeI 4816 CATATG CA^TATG
NspV 2707 TTCGAA TT^CGAA
PmaCI 2308 CACGTG CAC^GTG
PmlI 2308 CACGTG CAC^GTG
SfuI 2707 TTCGAA TT^CGAA
StuI 4656 AGGCCT AGG^CCT
XbaI 3300 TCTAGA T^CTAGA
XmnI 706 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 231 AAATTT [GA]^AATT[CT]
2677 AAATTT
AflIII 501 ACATGT A^C[GA][CT]GT
530 ACGTGT
ApoI 231 AAATTT [GA]^AATT[CT]
2677 AAATTT
AspI 749 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2244 GACCAAGTC
BcgI 109 CGAGAAGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
468 GCAGTGTCATCG
BciVI 1278 GGATAC GTATCC(6/5)
5217 GTATCC
BglII 3323 AGATCT A^GATCT
4352 AGATCT
BlpI 3251 GCTGAGC GC^T[ACGT]AGC
4524 GCTGAGC
Bpu1102I 3251 GCTGAGC GC^T[ACGT]AGC
4524 GCTGAGC
BsaAI 2308 CACGTG [CT]AC^GT[GA]
5030 TACGTG
BsiEI 11 CGACCG CG[GA][CT]^CG
202 CGGCCG
BssHII 123 GCGCGC G^CGCGC
125 GCGCGC
BstAPI 910 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2979 GCAGTGCCTGC
CelII 3251 GCTGAGC GC^T[ACGT]AGC
4524 GCTGAGC
EarI 1039 CTCTTC CTCTTC(1/4)
4458 CTCTTC
Ksp632I 1039 CTCTTC CTCTTC(1/4)
4458 CTCTTC
PflFI 749 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2244 GACCAAGTC
SacI 1722 GAGCTC GAGCT^C
3644 GAGCTC
SexAI 750 ACCAGGT A^CC[AT]GGT
3518 ACCTGGT
SfiI 1319 GGCCCTATAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2016 GGCCTTCCTGGCC
SmaI 184 CCCGGG CCC^GGG
2253 CCCGGG
SphI 2068 GCATGC GCATG^C
4674 GCATGC
TaqI 477 TCGA T^CGA
2708 TCGA
Tth111I 749 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2244 GACCAAGTC
TthHB8I 477 TCGA T^CGA
2708 TCGA
XmaCI 184 CCCGGG C^CCGGG
2253 CCCGGG
XmaI 184 CCCGGG C^CCGGG
2253 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspMI BsrFI BsrGI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XhoII