##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
Acc65I AclI AgeI Aor51HI AscI
Asp718I AviII AvrII BbeI BbvCI
BcgI BclI BlnI BsaBI BsgI
BsiWI BspDI BspHI Bst1107I BstBI
BstZ17I ClaI CpoI DraI DraIII
Eco47III EcoRV FbaI FseI FspI
HincII HindII HpaI KasI KpnI
MamI MluI NarI NheI NotI
NruI NspV PinAI PmeI PshAI
Psp1406I PvuI RcaI RsrII SalI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AatII 4594 GACGTC GACGT^C
AccIII 3588 TCCGGA T^CCGGA
AcyI 4594 GACGTC G[GA]^CG[CT]C
AflII 4912 CTTAAG C^TTAAG
AhdI 1215 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3520 GTGCAC G^TGCAC
AocI 2366 CCTCAGG CC^T[ACGT]AGG
ApaLI 3520 GTGCAC G^TGCAC
AspEI 1215 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4594 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 4036 TGGCCA TGG^CCA
BamHI 2671 GGATCC G^GATCC
BbrPI 4846 CACGTG CAC^GTG
BciVI 4096 GGATAC GTATCC(6/5)
BfrI 4912 CTTAAG C^TTAAG
BglII 2597 AGATCT A^GATCT
BsaAI 4846 CACGTG [CT]AC^GT[GA]
BsaHI 4594 GACGTC G[GA]^CG[CT]C
BseAI 3588 TCCGGA T^CCGGA
BsiEI 1 CGGCCG CG[GA][CT]^CG
BsmBI 4613 GAGACG CGTCTC(1/5)
BspEI 3588 TCCGGA T^CCGGA
BspLU11I 4563 ACATGT A^CATGT
BsrBI 30 CCGCTC CCGCTC(-3/-3)
BsrFI 2281 GCCGGC [GA]^CCGG[CT]
BssHII 108 GCGCGC G^CGCGC
BssSI 1752 CACGAG CACGAG(-5/-1)
BstXI 3674 CCAGCCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2366 CCTCAGG CC^T[ACGT]AGG
Cfr10I 2281 GCCGGC [GA]^CCGG[CT]
EagI 1 CGGCCG C^GGCCG
Eam1105I 1215 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1 CGGCCG C^GGCCG
Eco52I 1 CGGCCG C^GGCCG
Eco81I 2366 CCTCAGG CC^T[ACGT]AGG
EcoT22I 3278 ATGCAT ATGCA^T
HaeII 503 AGCGCC [GA]GCGC^[CT]
Hin1I 4594 GACGTC G[GA]^CG[CT]C
MluNI 4036 TGGCCA TGG^CCA
MroI 3588 TCCGGA T^CCGGA
MscI 4036 TGGCCA TGG^CCA
NaeI 2281 GCCGGC GCC^GGC
NdeI 1959 CATATG CA^TATG
NgoMIV 2281 GCCGGC G^CCGGC
NsiI 3278 ATGCAT ATGCA^T
PacI 5598 TTAATTAA TTAAT^TAA
PaeR7I 4080 CTCGAG C^TCGAG
PflFI 4594 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4846 CACGTG CAC^GTG
PmlI 4846 CACGTG CAC^GTG
SapI 5018 GCTCTTC GCTCTTC(1/4)
SbfI 3448 CCTGCAGG CCTGCA^GG
SmaI 3578 CCCGGG CCC^GGG
SphI 622 GCATGC GCATG^C
Sse8387I 3448 CCTGCAGG CCTGCA^GG
SspI 4733 AATATT AAT^ATT
Tth111I 4594 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2488 TCTAGA T^CTAGA
XhoI 4080 CTCGAG C^TCGAG
XmaCI 3578 CCCGGG C^CCGGG
XmaI 3578 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 2218 GTCTAC GT^[AC][GT]AC
4135 GTAGAC
ApaI 4437 GGGCCC GGGCC^C
4494 GGGCCC
AseI 1567 ATTAAT AT^TAAT
3777 ATTAAT
AsnI 1567 ATTAAT AT^TAAT
3777 ATTAAT
AvaI 3578 CCCGGG C^[CT]CG[GA]G
4080 CTCGAG
BcnI 3578 CCCGG CC^[GC]GG
3579 CCGGG
BglI 3372 GCCAAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4569 GCCCATCAGGC
BlpI 629 GCTCAGC GC^T[ACGT]AGC
2201 GCTCAGC
Bpu1102I 629 GCTCAGC GC^T[ACGT]AGC
2201 GCTCAGC
BsaWI 2759 ACCGGA [AT]^CCGG[AT]
3588 TCCGGA
BsoBI 3578 CCCGGG C^[CT]CG[GA]G
4080 CTCGAG
CelII 629 GCTCAGC GC^T[ACGT]AGC
2201 GCTCAGC
DrdI 1825 GACTCCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2905 GACTCCGTGGTC
EaeI 1 CGGCCG [CT]^GGCC[GA]
4036 TGGCCA
EcoRI 2558 GAATTC G^AATTC
3862 GAATTC
HgaI 73 GACGC GACGC(5/10)
493 GACGC
HindIII 954 AAGCTT A^AGCTT
4256 AAGCTT
KspI 14 CCGCGG CCGC^GG
22 CCGCGG
MfeI 467 CAATTG C^AATTG
4772 CAATTG
MunI 467 CAATTG C^AATTG
4772 CAATTG
NciI 3578 CCCGG CC^[GC]GG
3579 CCGGG
PflMI 1194 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4427 CCAGCAGTTGG
PshBI 1567 ATTAAT AT^TAAT
3777 ATTAAT
SacI 2723 GAGCTC GAGCT^C
5251 GAGCTC
SacII 14 CCGCGG CCGC^GG
22 CCGCGG
SexAI 2073 ACCAGGT A^CC[AT]GGT
5324 ACCAGGT
Van91I 1194 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4427 CCAGCAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BanI BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspMI BsrDI BsrGI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EarI EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI