##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI Alw44I Aor51HI
ApaLI AscI Asp700I Asp718I AspI
AviII AvrII BbeI BbrPI BbvCI
BglII BlnI BsaHI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BspLU11I BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I Cfr10I
ClaI DraI DrdI EagI EclXI
Eco47III Eco52I FseI FspI HaeII
Hin1I HpaI KasI KpnI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
StuI SwaI TfiI Tth111I XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 1028 GTCTAC GT^[AC][GT]AC
AflII 1105 CTTAAG C^TTAAG
ApaI 1816 GGGCCC GGGCC^C
AseI 1385 ATTAAT AT^TAAT
AsnI 1385 ATTAAT AT^TAAT
BamHI 1759 GGATCC G^GATCC
BcgI 1745 CGAGAGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2671 GTATCC GTATCC(6/5)
BclI 2007 TGATCA T^GATCA
BfrI 1105 CTTAAG C^TTAAG
BglI 872 GCCCCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 218 CACGTA [CT]AC^GT[GA]
BsaBI 115 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 44 GGTCTC GGTCTC(1/5)
BsmBI 1835 GAGACG CGTCTC(1/5)
BsmI 1825 GCATTC GAATGC(1/-1)
BsrBI 2310 CCGCTC CCGCTC(-3/-3)
BstEII 1549 GGTTACC G^GT[ACGT]ACC
BstPI 1549 GGTTACC G^GT[ACGT]ACC
CpoI 1839 CGGACCG CG^G[AT]CCG
DraIII 630 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1470 CCACGG C^C[GA][CT]GG
EcoO65I 1549 GGTTACC G^GT[ACGT]ACC
EcoRI 490 GAATTC G^AATTC
EcoRV 1961 GATATC GAT^ATC
EcoT22I 659 ATGCAT ATGCA^T
FbaI 2007 TGATCA T^GATCA
HgaI 69 GCGTC GACGC(5/10)
MamI 115 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 659 ATGCAT ATGCA^T
PaeR7I 1018 CTCGAG C^TCGAG
PshBI 1385 ATTAAT AT^TAAT
PvuII 911 CAGCTG CAG^CTG
RsrII 1839 CGGACCG CG^G[AT]CCG
ScaI 1800 AGTACT AGT^ACT
SexAI 1173 ACCAGGT A^CC[AT]GGT
SspI 1350 AATATT AAT^ATT
XcmI 309 CCAGGGATTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1018 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 490 GAATTC [GA]^AATT[CT]
1714 AAATTT
AhdI 743 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1619 GACCTAAGGTC
ApoI 490 GAATTC [GA]^AATT[CT]
1714 AAATTT
AspEI 743 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1619 GACCTAAGGTC
BalI 1191 TGGCCA TGG^CCA
1467 TGGCCA
BanI 940 GGCACC G^G[CT][GA]CC
1334 GGTGCC
Bsp1407I 409 TGTACA T^GTACA
1696 TGTACA
BspMI 683 ACCTGC ACCTGC(4/8)
80 GCAGGT
BsrGI 409 TGTACA T^GTACA
1696 TGTACA
CfoI 694 GCGC GCG^C
888 GCGC
EaeI 1191 TGGCCA [CT]^GGCC[GA]
1467 TGGCCA
Eam1105I 743 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1619 GACCTAAGGTC
EcoNI 386 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1609 CCTCAGGCAGG
EcoT14I 1449 CCAAGG C^C[AT][AT]GG
1707 CCTTGG
HhaI 694 GCGC GCG^C
888 GCGC
HinP1I 694 GCGC G^CGC
888 GCGC
HincII 1073 GTTGAC GT[CT]^[GA]AC
1733 GTCAAC
HindII 1073 GTTGAC GT[CT]^[GA]AC
1733 GTCAAC
HindIII 927 AAGCTT A^AGCTT
2387 AAGCTT
MaeII 219 ACGT A^CGT
1483 ACGT
MluNI 1191 TGGCCA TGG^CCA
1467 TGGCCA
MscI 1191 TGGCCA TGG^CCA
1467 TGGCCA
MspA1I 911 CAGCTG C[AC]G^C[GT]G
1951 CCGCTG
PflMI 419 CCAAATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2364 CCAGATTTTGG
PstI 472 CTGCAG CTGCA^G
1277 CTGCAG
SacI 1293 GAGCTC GAGCT^C
1918 GAGCTC
SmaI 31 CCCGGG CCC^GGG
2100 CCCGGG
SspBI 409 TGTACA T^GTACA
1696 TGTACA
StyI 1449 CCAAGG C^C[AT][AT]GG
1707 CCTTGG
Van91I 419 CCAAATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2364 CCAGATTTTGG
XmaCI 31 CCCGGG C^CCGGG
2100 CCCGGG
XmaI 31 CCCGGG C^CCGGG
2100 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AspHI AvaI AvaII BanII
BbsI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII Cfr13I
DdeI DpnII DraII EarI Eco81I
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII