##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
AscI AseI AsnI AspEI AviII
BbeI BbrPI BciVI BsaBI BseAI
BsiWI Bsp1407I BspDI BspEI BsrBI
BsrGI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI Eam1105I
EcoO65I EcoRV FspI HpaI KasI
KspI MamI MroI NarI NdeI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SapI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SspBI SspI
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 878 GGTACC G^GTACC
AccI 752 GTCGAC GT^[AC][GT]AC
AcyI 1229 GACGCC G[GA]^CG[CT]C
AflII 4406 CTTAAG C^TTAAG
AocI 802 CCTGAGG CC^T[ACGT]AGG
Aor51HI 3140 AGCGCT AGC^GCT
ApaI 201 GGGCCC GGGCC^C
Asp700I 4521 GAATAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 878 GGTACC G^GTACC
AspI 154 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3614 CCTAGG C^CTAGG
BamHI 1304 GGATCC G^GATCC
BcgI 421 GCACCCAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4406 CTTAAG C^TTAAG
BglI 4142 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3614 CCTAGG C^CTAGG
BsaAI 5517 CACGTA [CT]AC^GT[GA]
BsaHI 1229 GACGCC G[GA]^CG[CT]C
BsaWI 1225 ACCGGA [AT]^CCGG[AT]
BsmBI 346 GAGACG CGTCTC(1/5)
BsmI 4411 GAATGC GAATGC(1/-1)
BspLU11I 3860 ACATGT A^CATGT
BssHII 131 GCGCGC G^CGCGC
BstAPI 5033 GCAATTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 802 CCTGAGG CC^T[ACGT]AGG
DrdI 4007 GACTCCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3140 AGCGCT AGC^GCT
Eco81I 802 CCTGAGG CC^T[ACGT]AGG
EcoT22I 4718 ATGCAT ATGCA^T
FseI 2 GGCCGGCC GGCCGG^CC
Hin1I 1229 GACGCC G[GA]^CG[CT]C
HindIII 4192 AAGCTT A^AGCTT
KpnI 878 GGTACC GGTAC^C
MluI 1162 ACGCGT A^CGCGT
NcoI 339 CCATGG C^CATGG
NsiI 4718 ATGCAT ATGCA^T
PflFI 154 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3668 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2001 GAGCTC GAGCT^C
SalI 752 GTCGAC G^TCGAC
SbfI 5199 CCTGCAGG CCTGCA^GG
SexAI 2049 ACCTGGT A^CC[AT]GGT
SphI 3692 GCATGC GCATG^C
Sse8387I 5199 CCTGCAGG CCTGCA^GG
Tth111I 154 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 4521 GAATAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Alw44I 1132 GTGCAC G^TGCAC
3854 GTGCAC
ApaLI 1132 GTGCAC G^TGCAC
3854 GTGCAC
AvaI 227 CCCGAG C^[CT]CG[GA]G
2150 CCCGAG
BalI 782 TGGCCA TGG^CCA
3914 TGGCCA
BclI 1277 TGATCA T^GATCA
5229 TGATCA
BsoBI 227 CCCGAG C^[CT]CG[GA]G
2150 CCCGAG
BspHI 1688 TCATGA T^CATGA
4128 TCATGA
BssSI 1318 CTCGTG CACGAG(-5/-1)
5792 CTCGTG
DraIII 2257 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
2732 CACTAAGTG
EcoNI 1089 CCTCTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2896 CCTCCCAAAGG
FauI 177 CCCGC CCCGC(4/6)
222 CCCGC
FbaI 1277 TGATCA T^GATCA
5229 TGATCA
HgaI 348 GACGC GACGC(5/10)
1229 GACGC
MfeI 4294 CAATTG C^AATTG
5666 CAATTG
MluNI 782 TGGCCA TGG^CCA
3914 TGGCCA
MscI 782 TGGCCA TGG^CCA
3914 TGGCCA
MunI 4294 CAATTG C^AATTG
5666 CAATTG
NheI 2686 GCTAGC G^CTAGC
5637 GCTAGC
PflMI 1233 CCATGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1752 CCAGGCTCTGG
RcaI 1688 TCATGA T^CATGA
4128 TCATGA
SmlI 486 CTTGAG C^T[CT][GA]AG
4406 CTTAAG
Van91I 1233 CCATGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1752 CCAGGCTCTGG
XbaI 2548 TCTAGA T^CTAGA
3889 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII