##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AspEI
AspI AviII BalI BbrPI BcgI
BclI BcnI BfrI BglI BsaBI
BsiEI BspDI BsrFI BssHII BssSI
BstBI BstXI Cfr10I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoT22I FbaI FseI FspI HpaI
KspI MamI MluI MluNI MscI
NaeI NciI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SexAI SfiI SfuI SgrAI
SmaI SpeI Sse8387I StuI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 2516 GGTACC G^GTACC
AccIII 36 TCCGGA T^CCGGA
AcyI 3161 GGCGCC G[GA]^CG[CT]C
ApaI 111 GGGCCC GGGCC^C
Asp718I 2516 GGTACC G^GTACC
AvrII 5074 CCTAGG C^CTAGG
BamHI 1791 GGATCC G^GATCC
BbeI 3161 GGCGCC GGCGC^C
BlnI 5074 CCTAGG C^CTAGG
BsaHI 3161 GGCGCC G[GA]^CG[CT]C
BsaWI 36 TCCGGA [AT]^CCGG[AT]
BseAI 36 TCCGGA T^CCGGA
BsiWI 4719 CGTACG C^GTACG
BsmBI 2144 GAGACG CGTCTC(1/5)
Bsp1407I 4051 TGTACA T^GTACA
BspEI 36 TCCGGA T^CCGGA
BspHI 3008 TCATGA T^CATGA
BsrBI 57 GAGCGG CCGCTC(-3/-3)
BsrGI 4051 TGTACA T^GTACA
BstEII 5563 GGTCACC G^GT[ACGT]ACC
BstPI 5563 GGTCACC G^GT[ACGT]ACC
DraIII 475 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1931 GACACCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 12 CGGCCA [CT]^GGCC[GA]
EcoO65I 5563 GGTCACC G^GT[ACGT]ACC
EcoRV 810 GATATC GAT^ATC
HapII 37 CCGG C^CGG
Hin1I 3161 GGCGCC G[GA]^CG[CT]C
HincII 4086 GTTGAC GT[CT]^[GA]AC
HindII 4086 GTTGAC GT[CT]^[GA]AC
HpaII 37 CCGG C^CGG
KasI 3161 GGCGCC G^GCGCC
KpnI 2516 GGTACC GGTAC^C
MfeI 2746 CAATTG C^AATTG
MroI 36 TCCGGA T^CCGGA
MspI 37 CCGG C^CGG
MunI 2746 CAATTG C^AATTG
NarI 3161 GGCGCC GG^CGCC
PshAI 1543 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3008 TCATGA T^CATGA
SapI 877 GAAGAGC GCTCTTC(1/4)
SfoI 3161 GGCGCC GGC^GCC
SspBI 4051 TGTACA T^GTACA
SwaI 6631 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 10 CGCG CG^CG
108 CGCG
AocI 3165 CCTGAGG CC^T[ACGT]AGG
4036 CCTCAGG
AspHI 3267 GAGCAC G[AT]GC[AT]^C
4295 GAGCAC
AvaI 54 CCCGAG C^[CT]CG[GA]G
82 CCCGAG
BanII 111 GGGCCC G[GA]GC[CT]^C
142 GAGCCC
BlpI 426 GCTCAGC GC^T[ACGT]AGC
3459 GCTTAGC
Bpu1102I 426 GCTCAGC GC^T[ACGT]AGC
3459 GCTTAGC
BsiHKAI 3267 GAGCAC G[AT]GC[AT]^C
4295 GAGCAC
BsoBI 54 CCCGAG C^[CT]CG[GA]G
82 CCCGAG
BstUI 10 CGCG CG^CG
108 CGCG
Bsu36I 3165 CCTGAGG CC^T[ACGT]AGG
4036 CCTCAGG
CelII 426 GCTCAGC GC^T[ACGT]AGC
3459 GCTTAGC
DraII 743 AGGTCCT [GA]G^G[ACGT]CC[CT]
3997 AGGACCT
Eco81I 3165 CCTGAGG CC^T[ACGT]AGG
4036 CCTCAGG
EcoO109I 743 AGGTCCT [GA]G^G[ACGT]CC[CT]
3997 AGGACCT
EcoRI 2915 GAATTC G^AATTC
6182 GAATTC
FauI 59 GCGGG CCCGC(4/6)
109 GCGGG
HgaI 2338 GACGC GACGC(5/10)
581 GCGTC
MvnI 10 CGCG CG^CG
108 CGCG
NdeI 2181 CATATG CA^TATG
5505 CATATG
PflMI 485 CCAGGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6153 CCAGTATTTGG
PpuMI 743 AGGTCCT [GA]G^G[AT]CC[CT]
3997 AGGACCT
ScaI 2781 AGTACT AGT^ACT
5137 AGTACT
SnaBI 3299 TACGTA TAC^GTA
3404 TACGTA
Van91I 485 CCAGGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6153 CCAGTATTTGG
XbaI 1128 TCTAGA T^CTAGA
5570 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AvaII BanI BbsI
BbvCI BbvI BciVI BfaI BglII
BmyI BpmI BpuAI BsaAI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspLU11I
BspMI BsrDI BsrI BssKI Bst1107I
BstAPI BstF5I BstNI BstYI BstZ17I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI EarI EcoNI EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HhaI HinP1I HindIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NcoI
NdeII NheI NlaIII NlaIV NspI
PleI PshBI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI