##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccIII AcyI AflIII AgeI
AocI Aor51HI AscI AspI AviII
AvrII BbeI BcgI BlnI BsaHI
BseAI BsiWI BspEI BspLU11I BsrFI
BssHII BstEII BstPI Bsu36I Cfr10I
CpoI DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV FseI
FspI HgaI Hin1I HpaI KasI
KspI MluI MroI NaeI NarI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI RsrII SacII SalI SapI
SbfI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
Van91I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 554 GGTACC G^GTACC
AccII 3778 CGCG CG^CG
AclI 1413 AACGTT AA^CGTT
AflII 5966 CTTAAG C^TTAAG
Alw44I 3153 GTGCAC G^TGCAC
ApaI 5447 GGGCCC GGGCC^C
ApaLI 3153 GTGCAC G^TGCAC
AseI 332 ATTAAT AT^TAAT
AsnI 332 ATTAAT AT^TAAT
Asp718I 554 GGTACC G^GTACC
BalI 5096 TGGCCA TGG^CCA
BamHI 4307 GGATCC G^GATCC
BanI 554 GGTACC G^G[CT][GA]CC
BbrPI 1796 CACGTG CAC^GTG
BclI 1072 TGATCA T^GATCA
BfrI 5966 CTTAAG C^TTAAG
BlpI 2231 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2231 GCTGAGC GC^T[ACGT]AGC
BsaAI 1796 CACGTG [CT]AC^GT[GA]
BsaWI 4450 ACCGGA [AT]^CCGG[AT]
BsiEI 5000 CGATCG CG[GA][CT]^CG
BspDI 396 ATCGAT AT^CGAT
BsrBI 4073 CCGCTC CCGCTC(-3/-3)
BssSI 5408 CACGAG CACGAG(-5/-1)
BstAPI 3080 GCAACCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 3778 CGCG CG^CG
CelII 2231 GCTGAGC GC^T[ACGT]AGC
ClaI 396 ATCGAT AT^CGAT
DraIII 1172 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 5096 TGGCCA [CT]^GGCC[GA]
EcoRI 1855 GAATTC G^AATTC
FbaI 1072 TGATCA T^GATCA
HaeII 5210 AGCGCC [GA]GCGC^[CT]
HindIII 2711 AAGCTT A^AGCTT
KpnI 554 GGTACC GGTAC^C
MfeI 1488 CAATTG C^AATTG
MluNI 5096 TGGCCA TGG^CCA
MscI 5096 TGGCCA TGG^CCA
MunI 1488 CAATTG C^AATTG
MvnI 3778 CGCG CG^CG
NcoI 3825 CCATGG C^CATGG
NspI 5798 GCATGT [GA]CATG^[CT]
PmaCI 1796 CACGTG CAC^GTG
PmlI 1796 CACGTG CAC^GTG
PshAI 3332 GACCCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 332 ATTAAT AT^TAAT
Psp1406I 1413 AACGTT AA^CGTT
PvuI 5000 CGATCG CGAT^CG
SacI 2234 GAGCTC GAGCT^C
ScaI 1432 AGTACT AGT^ACT
SexAI 363 ACCAGGT A^CC[AT]GGT
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AhdI 3577 GACTCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5381 GACCCTTGGTC
AlwNI 720 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
5473 CAGTTTCTG
AspEI 3577 GACTCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5381 GACCCTTGGTC
BbvCI 3671 CCTCAGC CCTCAGC(-5/-2)
3698 CCTCAGC
BsaBI 2111 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4308 GATCCAAATC
BsgI 2063 GTGCAG GTGCAG(16/14)
3087 GTGCAG
BsmBI 4581 CGTCTC CGTCTC(1/5)
1524 GAGACG
BsmI 135 GAATGC GAATGC(1/-1)
3052 GAATGC
Bsp1407I 2771 TGTACA T^GTACA
5670 TGTACA
BspHI 1699 TCATGA T^CATGA
5462 TCATGA
BsrGI 2771 TGTACA T^GTACA
5670 TGTACA
Bst1107I 1364 GTATAC GTA^TAC
6052 GTATAC
BstBI 3216 TTCGAA TT^CGAA
3565 TTCGAA
BstXI 364 CCAGGTATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4448 CCACCGGAGTGG
BstZ17I 1364 GTATAC GTA^TAC
6052 GTATAC
DraII 2520 AGGGCCT [GA]G^G[ACGT]CC[CT]
5447 GGGCCCT
DsaI 3825 CCATGG C^C[GA][CT]GG
5339 CCGTGG
Eam1105I 3577 GACTCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5381 GACCCTTGGTC
EcoO109I 2520 AGGGCCT [GA]G^G[ACGT]CC[CT]
5447 GGGCCCT
MamI 2111 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4308 GATCCAAATC
NdeI 3071 CATATG CA^TATG
6007 CATATG
NspV 3216 TTCGAA TT^CGAA
3565 TTCGAA
RcaI 1699 TCATGA T^CATGA
5462 TCATGA
SfuI 3216 TTCGAA TT^CGAA
3565 TTCGAA
SmaI 4614 CCCGGG CCC^GGG
4717 CCCGGG
SspBI 2771 TGTACA T^GTACA
5670 TGTACA
StuI 3237 AGGCCT AGG^CCT
5013 AGGCCT
XmaCI 4614 CCCGGG C^CCGGG
4717 CCCGGG
XmaI 4614 CCCGGG C^CCGGG
4717 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaI
AvaII BanII BbsI BbvI BciVI
BcnI BfaI BglI BglII BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EarI EcoNI EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NsiI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XhoII XmnI