##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvrII BamHI BbrPI
BcgI BfrI BlnI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BsrBI BssHII BssSI BstEII BstPI
Bsu36I ClaI CpoI DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoT22I FauI FseI
HpaI KpnI KspI MluI MroI
NcoI NdeI NotI NruI NsiI
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RsrII SacI SacII SapI SfiI
SgrAI SmaI SnaBI SpeI SphI
SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccII 34 CGCG CG^CG
AvaII 323 GGTCC G^G[AT]CC
AviII 5204 TGCGCA TGC^GCA
BalI 3201 TGGCCA TGG^CCA
BanI 1 GGCGCC G^G[CT][GA]CC
BbeI 1 GGCGCC GGCGC^C
BbvCI 4880 CCTCAGC CCTCAGC(-5/-2)
BciVI 2545 GGATAC GTATCC(6/5)
BclI 289 TGATCA T^GATCA
BcnI 53 CCCGG CC^[GC]GG
BglI 4043 GCCTCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1549 AGATCT A^GATCT
BlpI 1637 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1637 GCTGAGC GC^T[ACGT]AGC
BsaAI 3699 TACGTG [CT]AC^GT[GA]
BsgI 4115 GTGCAG GTGCAG(16/14)
BsmI 5201 GAATGC GAATGC(1/-1)
BspHI 3865 TCATGA T^CATGA
BspLU11I 4288 ACATGT A^CATGT
BspMI 2224 ACCTGC ACCTGC(4/8)
BsrFI 3105 GCCGGC [GA]^CCGG[CT]
Bst1107I 488 GTATAC GTA^TAC
BstBI 4023 TTCGAA TT^CGAA
BstUI 34 CGCG CG^CG
BstXI 2047 CCAGTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 488 GTATAC GTA^TAC
CelII 1637 GCTGAGC GC^T[ACGT]AGC
Cfr10I 3105 GCCGGC [GA]^CCGG[CT]
DraII 1613 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoNI 2563 CCTGTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1613 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 3083 GATATC GAT^ATC
FbaI 289 TGATCA T^GATCA
FspI 5204 TGCGCA TGC^GCA
HaeII 1 GGCGCC [GA]GCGC^[CT]
HgaI 35 GCGTC GACGC(5/10)
KasI 1 GGCGCC G^GCGCC
MluNI 3201 TGGCCA TGG^CCA
MscI 3201 TGGCCA TGG^CCA
MvnI 34 CGCG CG^CG
NaeI 3105 GCCGGC GCC^GGC
NarI 1 GGCGCC GG^CGCC
NciI 53 CCCGG CC^[GC]GG
NgoMIV 3105 GCCGGC G^CCGGC
NheI 4885 GCTAGC G^CTAGC
NspV 4023 TTCGAA TT^CGAA
PshAI 3929 GACTATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3865 TCATGA T^CATGA
SalI 3120 GTCGAC G^TCGAC
SbfI 4092 CCTGCAGG CCTGCA^GG
ScaI 2159 AGTACT AGT^ACT
SexAI 4354 ACCTGGT A^CC[AT]GGT
SfoI 1 GGCGCC GGC^GCC
SfuI 4023 TTCGAA TT^CGAA
Sse8387I 4092 CCTGCAGG CCTGCA^GG
StuI 1337 AGGCCT AGG^CCT
XbaI 1114 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AatII 1600 GACGTC GACGT^C
2634 GACGTC
AspHI 2996 GAGCAC G[AT]GC[AT]^C
5106 GAGCAC
BanII 1640 GAGCCC G[GA]GC[CT]^C
2528 GAGCCC
BsaBI 1544 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5063 GATTTTTATC
BsiHKAI 2996 GAGCAC G[AT]GC[AT]^C
5106 GAGCAC
BsrDI 4489 CATTGC GCAATG(2/0)
4205 GCAATG
BstAPI 3548 GCAAGGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4445 GCAGAAGCTGC
BstYI 1549 AGATCT [GA]^GATC[CT]
3163 AGATCC
EcoRI 179 GAATTC G^AATTC
4759 GAATTC
HapII 54 CCGG C^CGG
3106 CCGG
HindIII 302 AAGCTT A^AGCTT
2451 AAGCTT
HpaII 54 CCGG C^CGG
3106 CCGG
MamI 1544 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5063 GATTTTTATC
MfeI 779 CAATTG C^AATTG
999 CAATTG
MflI 1549 AGATCT [GA]^GATC[CT]
3163 AGATCC
MspA1I 3132 CAGCTG C[AC]G^C[GT]G
4053 CAGCTG
MspI 54 CCGG C^CGG
3106 CCGG
MunI 779 CAATTG C^AATTG
999 CAATTG
PaeR7I 164 CTCGAG C^TCGAG
4302 CTCGAG
PvuII 3132 CAGCTG CAG^CTG
4053 CAGCTG
XcmI 3069 CCAAAATGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3810 CCAACCCCCCTGTGG
XhoI 164 CTCGAG C^TCGAG
4302 CTCGAG
XhoII 1549 AGATCT [GA]^GATC[CT]
3163 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AvaI BbsI BbvI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraIII EaeI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HhaI Hin1I
HinP1I HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XmnI