##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AccIII AclI AflII AgeI Alw44I
Aor51HI ApaI ApaLI AscI Asp700I
AviII BbeI BcgI BfrI BsaBI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI BsrBI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI Eco47III EcoNI EcoO65I FseI
FspI KasI KspI MamI MfeI
MluI MroI MunI NarI NheI
NotI NruI NspV PaeR7I PinAI
PmeI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI Sse8387I SwaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 2669 GGTACC G^GTACC
AccI 5744 GTCTAC GT^[AC][GT]AC
AccII 137 CGCG CG^CG
AhdI 4974 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 2669 GGTACC G^GTACC
AspEI 4974 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5200 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3506 CCTAGG C^CTAGG
BamHI 4257 GGATCC G^GATCC
BbvCI 4853 CCTCAGC CCTCAGC(-5/-2)
BciVI 1911 GGATAC GTATCC(6/5)
BclI 5728 TGATCA T^GATCA
BcnI 2087 CCCGG CC^[GC]GG
BglI 330 GCCACAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3512 AGATCT A^GATCT
BlnI 3506 CCTAGG C^CTAGG
BsiEI 184 CGGCCG CG[GA][CT]^CG
BsmI 1756 GCATTC GAATGC(1/-1)
BsrFI 222 GCCGGC [GA]^CCGG[CT]
BstUI 137 CGCG CG^CG
CfoI 360 GCGC GCG^C
Cfr10I 222 GCCGGC [GA]^CCGG[CT]
DraIII 301 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 184 CGGCCG C^GGCCG
Eam1105I 4974 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 184 CGGCCG C^GGCCG
Eco52I 184 CGGCCG C^GGCCG
EcoRI 4668 GAATTC G^AATTC
EcoT22I 3675 ATGCAT ATGCA^T
FbaI 5728 TGATCA T^GATCA
HaeII 359 GGCGCT [GA]GCGC^[CT]
HhaI 360 GCGC GCG^C
HinP1I 360 GCGC G^CGC
KpnI 2669 GGTACC GGTAC^C
MvnI 137 CGCG CG^CG
NaeI 222 GCCGGC GCC^GGC
NciI 2087 CCCGG CC^[GC]GG
NgoMIV 222 GCCGGC G^CCGGC
NsiI 3675 ATGCAT ATGCA^T
PacI 3083 TTAATTAA TTAAT^TAA
PflFI 5200 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2268 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 3977 GACTAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 239 GAGCTC GAGCT^C
SapI 1086 GAAGAGC GCTCTTC(1/4)
ScaI 905 AGTACT AGT^ACT
SexAI 3798 ACCTGGT A^CC[AT]GGT
SpeI 3978 ACTAGT A^CTAGT
SphI 110 GCATGC GCATG^C
TaqI 404 TCGA T^CGA
Tth111I 5200 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 404 TCGA T^CGA
XbaI 4993 TCTAGA T^CTAGA
XcmI 616 CCAGCTCTGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 103 GACGTC GACGT^C
2315 GACGTC
AlwNI 3121 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
5011 CAGTTTCTG
AocI 4778 CCTGAGG CC^T[ACGT]AGG
5001 CCTTAGG
AseI 968 ATTAAT AT^TAAT
1362 ATTAAT
AsnI 968 ATTAAT AT^TAAT
1362 ATTAAT
AvaI 40 CCCGAG C^[CT]CG[GA]G
115 CTCGGG
AvaII 2269 GGTCC G^G[AT]CC
4477 GGACC
BbrPI 1013 CACGTG CAC^GTG
3572 CACGTG
BsoBI 40 CCCGAG C^[CT]CG[GA]G
115 CTCGGG
BspHI 763 TCATGA T^CATGA
3423 TCATGA
BspMI 2073 ACCTGC ACCTGC(4/8)
5079 GCAGGT
BstAPI 684 GCAAATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3189 GCATACTTTGC
BstXI 4312 CCAGGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5271 CCAAAGTGCTGG
Bsu36I 4778 CCTGAGG CC^T[ACGT]AGG
5001 CCTTAGG
DrdI 2201 GACAGGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3732 GACTGAATAGTC
DsaI 85 CCATGG C^C[GA][CT]GG
4224 CCATGG
Eco81I 4778 CCTGAGG CC^T[ACGT]AGG
5001 CCTTAGG
EcoRV 1467 GATATC GAT^ATC
2600 GATATC
FauI 55 CCCGC CCCGC(4/6)
135 CCCGC
HgaI 4571 GACGC GACGC(5/10)
180 GCGTC
HpaI 2565 GTTAAC GTT^AAC
3877 GTTAAC
NcoI 85 CCATGG C^CATGG
4224 CCATGG
NdeI 1492 CATATG CA^TATG
2975 CATATG
PflMI 1 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
320 CCAGATGGTGG
PmaCI 1013 CACGTG CAC^GTG
3572 CACGTG
PmlI 1013 CACGTG CAC^GTG
3572 CACGTG
PshBI 968 ATTAAT AT^TAAT
1362 ATTAAT
PstI 31 CTGCAG CTGCA^G
855 CTGCAG
RcaI 763 TCATGA T^CATGA
3423 TCATGA
SnaBI 696 TACGTA TAC^GTA
1176 TACGTA
SspI 3414 AATATT AAT^ATT
5880 AATATT
Van91I 1 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
320 CCAGATGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI ApoI AspHI BalI
BanI BanII BbsI BbvI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspLU11I BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I CelII Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII Hin1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII