##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I ApaLI AscI
Asp718I AvaI AviII AvrII BamHI
BcgI BfrI BglI BlnI BsaWI
BseAI BsiEI BsiWI BsoBI BspEI
BssHII BssSI Bst1107I BstZ17I CpoI
DraIII DrdI EagI EclXI Eco52I
FseI FspI HgaI KpnI MluI
MroI NcoI NotI NruI PacI
PaeR7I PinAI PmeI PpuMI PshAI
Psp1406I PvuI RsrII SacI SalI
SapI SbfI SexAI SfiI SmaI
SnaBI SpeI Sse8387I XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AcyI 91 GGCGCC G[GA]^CG[CT]C
AhdI 1791 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3210 CCTCAGG CC^T[ACGT]AGG
Aor51HI 3173 AGCGCT AGC^GCT
ApaI 47 GGGCCC GGGCC^C
AspEI 1791 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 669 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1327 GGACC G^G[AT]CC
BbeI 91 GGCGCC GGCGC^C
BbrPI 2205 CACGTG CAC^GTG
BbvCI 2994 GCTGAGG CCTCAGC(-5/-2)
BciVI 1415 GGATAC GTATCC(6/5)
BclI 3463 TGATCA T^GATCA
BsaAI 2205 CACGTG [CT]AC^GT[GA]
BsaHI 91 GGCGCC G[GA]^CG[CT]C
BsmBI 1482 CGTCTC CGTCTC(1/5)
BspDI 1102 ATCGAT AT^CGAT
BspLU11I 4803 ACATGT A^CATGT
BspMI 163 ACCTGC ACCTGC(4/8)
BstBI 2362 TTCGAA TT^CGAA
BstEII 1936 GGTCACC G^GT[ACGT]ACC
BstPI 1936 GGTCACC G^GT[ACGT]ACC
BstXI 406 CCACATTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3210 CCTCAGG CC^T[ACGT]AGG
ClaI 1102 ATCGAT AT^CGAT
Eam1105I 1791 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3173 AGCGCT AGC^GCT
Eco81I 3210 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1936 GGTCACC G^GT[ACGT]ACC
EcoRI 4784 GAATTC G^AATTC
EcoT22I 4019 ATGCAT ATGCA^T
FbaI 3463 TGATCA T^GATCA
Hin1I 91 GGCGCC G[GA]^CG[CT]C
HindIII 4378 AAGCTT A^AGCTT
HpaI 4096 GTTAAC GTT^AAC
KasI 91 GGCGCC G^GCGCC
KspI 205 CCGCGG CCGC^GG
NarI 91 GGCGCC GG^CGCC
NdeI 3005 CATATG CA^TATG
NsiI 4019 ATGCAT ATGCA^T
NspV 2362 TTCGAA TT^CGAA
PflFI 669 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1963 CCAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2205 CACGTG CAC^GTG
PmlI 2205 CACGTG CAC^GTG
SacII 205 CCGCGG CCGC^GG
SfoI 91 GGCGCC GGC^GCC
SfuI 2362 TTCGAA TT^CGAA
SgrAI 78 CACCGGCG C[GA]^CCGG[CT]G
SphI 3623 GCATGC GCATG^C
StuI 1252 AGGCCT AGG^CCT
SwaI 4115 ATTTAAAT ATTT^AAAT
Tth111I 669 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1963 CCAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 2310 ACACGT A^C[GA][CT]GT
4803 ACATGT
AlwNI 2433 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
5279 CAGTGGCTG
BalI 244 TGGCCA TGG^CCA
3818 TGGCCA
BanI 91 GGCGCC G^G[CT][GA]CC
1242 GGCACC
BanII 47 GGGCCC G[GA]GC[CT]^C
5240 GGGCTC
BcnI 16 CCGGG CC^[GC]GG
113 CCGGG
BglII 2516 AGATCT A^GATCT
5061 AGATCT
BsaBI 1059 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4361 GATTCCAATC
BsmI 2365 GAATGC GAATGC(1/-1)
4021 GCATTC
BstAPI 1497 GCAGATATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5278 GCAGTGGCTGC
DraII 46 AGGGCCC [GA]G^G[ACGT]CC[CT]
47 GGGCCCT
DsaI 205 CCGCGG C^C[GA][CT]GG
241 CCGTGG
EaeI 244 TGGCCA [CT]^GGCC[GA]
3818 TGGCCA
EcoO109I 46 AGGGCCC [GA]G^G[ACGT]CC[CT]
47 GGGCCCT
EcoRV 453 GATATC GAT^ATC
1026 GATATC
EcoT14I 1208 CCAAGG C^C[AT][AT]GG
2410 CCTTGG
HaeII 91 GGCGCC [GA]GCGC^[CT]
3173 AGCGCT
MamI 1059 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4361 GATTCCAATC
MfeI 4834 CAATTG C^AATTG
5965 CAATTG
MluNI 244 TGGCCA TGG^CCA
3818 TGGCCA
MscI 244 TGGCCA TGG^CCA
3818 TGGCCA
MunI 4834 CAATTG C^AATTG
5965 CAATTG
NaeI 167 GCCGGC GCC^GGC
3627 GCCGGC
NciI 16 CCGGG CC^[GC]GG
113 CCGGG
NgoMIV 167 GCCGGC G^CCGGC
3627 GCCGGC
NspI 3623 GCATGC [GA]CATG^[CT]
4803 ACATGT
PstI 1459 CTGCAG CTGCA^G
5842 CTGCAG
SmlI 3414 CTTGAG C^T[CT][GA]AG
5895 CTTGAG
StyI 1208 CCAAGG C^C[AT][AT]GG
2410 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI Asp700I
AspHI BbsI BbvI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspHI BsrBI BsrDI BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI EarI
EcoNI EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NdeII NheI NlaIII NlaIV PleI
PshBI PvuII RcaI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SspBI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XhoII XmnI