##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AocI AscI AseI AsnI AviII
AvrII BalI BamHI BbeI BbrPI
BcgI BclI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmI
BspDI BspEI BsrBI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HpaI KasI KspI MaeII
MamI MfeI MflI MluI MluNI
MroI MscI MunI NaeI NarI
NcoI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspI
SwaI XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 310 GGTACC G^GTACC
AccI 108 GTAGAC GT^[AC][GT]AC
AflIII 2361 ACATGT A^C[GA][CT]GT
AhdI 1994 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2365 GTGCAC G^TGCAC
AlwNI 1134 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 839 AGCGCT AGC^GCT
ApaI 1263 GGGCCC GGGCC^C
ApaLI 2365 GTGCAC G^TGCAC
Asp700I 2512 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 310 GGTACC G^GTACC
AspEI 1994 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1640 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 247 CTCGGG C^[CT]CG[GA]G
BciVI 2051 GGATAC GTATCC(6/5)
BpmI 1666 CTGGAG CTGGAG(16/14)
BsmBI 2305 CGTCTC CGTCTC(1/5)
BsoBI 247 CTCGGG C^[CT]CG[GA]G
BspHI 505 TCATGA T^CATGA
BspLU11I 2361 ACATGT A^CATGT
BsrDI 567 CATTGC GCAATG(2/0)
EaeI 1916 CGGCCA [CT]^GGCC[GA]
Eam1105I 1994 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 839 AGCGCT AGC^GCT
HaeII 839 AGCGCT [GA]GCGC^[CT]
KpnI 310 GGTACC GGTAC^C
NheI 2217 GCTAGC G^CTAGC
PflFI 1640 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 301 CCAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1253 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1456 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 505 TCATGA T^CATGA
SapI 2589 GCTCTTC GCTCTTC(1/4)
SbfI 72 CCTGCAGG CCTGCA^GG
ScaI 1565 AGTACT AGT^ACT
Sse8387I 72 CCTGCAGG CCTGCA^GG
Tth111I 1640 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 301 CCAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1154 TCTAGA T^CTAGA
XcmI 2144 CCATGAACTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2512 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 27 CGCG CG^CG
43 CGCG
AflII 1479 CTTAAG C^TTAAG
2554 CTTAAG
AlwI 1172 GATCC GGATC(4/5)
2626 GGATC
AspHI 2365 GTGCAC G[AT]GC[AT]^C
2587 GTGCTC
AvaII 1254 GGTCC G^G[AT]CC
2101 GGACC
BanII 778 GAGCCC G[GA]GC[CT]^C
1263 GGGCCC
BcnI 1914 CCCGG CC^[GC]GG
2623 CCGGG
BfrI 1479 CTTAAG C^TTAAG
2554 CTTAAG
BsiHKAI 2365 GTGCAC G[AT]GC[AT]^C
2587 GTGCTC
Bsp1407I 1163 TGTACA T^GTACA
2346 TGTACA
BsrGI 1163 TGTACA T^GTACA
2346 TGTACA
BstAPI 1133 GCAGAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2525 GCAGATTTTGC
BstUI 27 CGCG CG^CG
43 CGCG
EcoT14I 1579 CCAAGG C^C[AT][AT]GG
1971 CCAAGG
FauI 1865 CCCGC CCCGC(4/6)
28 GCGGG
HapII 1915 CCGG C^CGG
2623 CCGG
HpaII 1915 CCGG C^CGG
2623 CCGG
MslI 1406 CATCGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2178 CATAGGGATG
MspA1I 880 CAGCTG C[AC]G^C[GT]G
1468 CAGCTG
MspI 1915 CCGG C^CGG
2623 CCGG
MvnI 27 CGCG CG^CG
43 CGCG
NciI 1914 CCCGG CC^[GC]GG
2623 CCGGG
NspI 742 ACATGC [GA]CATG^[CT]
2361 ACATGT
PstI 73 CTGCAG CTGCA^G
1140 CTGCAG
PvuII 880 CAGCTG CAG^CTG
1468 CAGCTG
SfcI 73 CTGCAG C^T[GA][CT]AG
1140 CTGCAG
SspBI 1163 TGTACA T^GTACA
2346 TGTACA
StuI 638 AGGCCT AGG^CCT
1009 AGGCCT
StyI 1579 CCAAGG C^C[AT][AT]GG
1971 CCAAGG
TfiI 450 GATTC G^A[AT]TC
1136 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BanI BbsI BbvCI BbvI BfaI
BmyI BpuAI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoNI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HhaI HinP1I HindIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I