##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AflII AgeI Alw44I
Aor51HI ApaLI AscI Asp718I AviII
BamHI BbeI BbrPI BbvCI BciVI
BfrI BglI BsaI BsiWI BsmBI
BsrBI BssHII Bst1107I BstEII BstPI
BstZ17I CpoI DrdI EagI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
FseI FspI HpaI KasI KpnI
KspI MfeI MunI NarI NheI
NotI NruI PacI PaeR7I PinAI
PmaCI PmeI PmlI PstI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SgrAI SmaI SpeI
Sse8387I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AccI 2205 GTAGAC GT^[AC][GT]AC
AccIII 1595 TCCGGA T^CCGGA
AclI 4206 AACGTT AA^CGTT
AhdI 2661 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3415 CCTCAGG CC^T[ACGT]AGG
ApaI 275 GGGCCC GGGCC^C
AspEI 2661 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1985 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 47 CCCGAG C^[CT]CG[GA]G
AvrII 2415 CCTAGG C^CTAGG
BalI 1385 TGGCCA TGG^CCA
BclI 1614 TGATCA T^GATCA
BcnI 273 CCGGG CC^[GC]GG
BglII 2821 AGATCT A^GATCT
BlnI 2415 CCTAGG C^CTAGG
BlpI 2715 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2715 GCTTAGC GC^T[ACGT]AGC
BsaAI 1086 TACGTA [CT]AC^GT[GA]
BsaBI 1753 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1595 TCCGGA [AT]^CCGG[AT]
BseAI 1595 TCCGGA T^CCGGA
BsiEI 3070 CGATCG CG[GA][CT]^CG
BsoBI 47 CCCGAG C^[CT]CG[GA]G
BspDI 2947 ATCGAT AT^CGAT
BspEI 1595 TCCGGA T^CCGGA
BspHI 3373 TCATGA T^CATGA
BstBI 509 TTCGAA TT^CGAA
BstXI 810 CCAGCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3415 CCTCAGG CC^T[ACGT]AGG
CelII 2715 GCTTAGC GC^T[ACGT]AGC
ClaI 2947 ATCGAT AT^CGAT
DraII 2570 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 2015 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2661 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3415 CCTCAGG CC^T[ACGT]AGG
EcoO109I 2570 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 3582 GAATTC G^AATTC
FbaI 1614 TGATCA T^GATCA
MamI 1753 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 4188 ACGCGT A^CGCGT
MluNI 1385 TGGCCA TGG^CCA
MroI 1595 TCCGGA T^CCGGA
MscI 1385 TGGCCA TGG^CCA
NaeI 2711 GCCGGC GCC^GGC
NciI 273 CCGGG CC^[GC]GG
NcoI 146 CCATGG C^CATGG
NdeI 1793 CATATG CA^TATG
NgoMIV 2711 GCCGGC G^CCGGC
NspV 509 TTCGAA TT^CGAA
PflFI 1985 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2570 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 823 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4206 AACGTT AA^CGTT
PvuI 3070 CGATCG CGAT^CG
PvuII 815 CAGCTG CAG^CTG
RcaI 3373 TCATGA T^CATGA
ScaI 4329 AGTACT AGT^ACT
SfuI 509 TTCGAA TT^CGAA
SnaBI 1086 TACGTA TAC^GTA
SphI 3904 GCATGC GCATG^C
StuI 981 AGGCCT AGG^CCT
SwaI 2951 ATTTAAAT ATTT^AAAT
Tth111I 1985 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 1 CGCG CG^CG
4189 CGCG
AseI 2355 ATTAAT AT^TAAT
3198 ATTAAT
AsnI 2355 ATTAAT AT^TAAT
3198 ATTAAT
AspHI 3122 GAGCAC G[AT]GC[AT]^C
3247 GAGCAC
BanII 50 GAGCCC G[GA]GC[CT]^C
275 GGGCCC
BbsI 4013 GAAGAC GAAGAC(2/6)
3171 GTCTTC
BcgI 3651 CGACTTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
109 GCAGGGGCTTCG
BpuAI 4013 GAAGAC GAAGAC(2/6)
3171 GTCTTC
BsiHKAI 3122 GAGCAC G[AT]GC[AT]^C
3247 GAGCAC
Bsp1407I 2148 TGTACA T^GTACA
4038 TGTACA
BspLU11I 2039 ACATGT A^CATGT
3517 ACATGT
BsrGI 2148 TGTACA T^GTACA
4038 TGTACA
BssSI 709 CACGAG CACGAG(-5/-1)
2343 CTCGTG
BstUI 1 CGCG CG^CG
4189 CGCG
CfoI 203 GCGC GCG^C
1835 GCGC
EaeI 1385 TGGCCA [CT]^GGCC[GA]
1823 CGGCCA
HaeII 202 AGCGCC [GA]GCGC^[CT]
1834 AGCGCC
HhaI 203 GCGC GCG^C
1835 GCGC
HinP1I 203 GCGC G^CGC
1835 GCGC
HincII 3046 GTCAAC GT[CT]^[GA]AC
3946 GTCAAC
HindII 3046 GTCAAC GT[CT]^[GA]AC
3946 GTCAAC
HindIII 2000 AAGCTT A^AGCTT
3938 AAGCTT
MvnI 1 CGCG CG^CG
4189 CGCG
PflMI 966 CCAGTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1902 CCATGAACTGG
PshBI 2355 ATTAAT AT^TAAT
3198 ATTAAT
SspBI 2148 TGTACA T^GTACA
4038 TGTACA
Van91I 966 CCAGTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1902 CCATGAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AvaII BanI BbvI BfaI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I Cfr10I Cfr13I DdeI DpnII
DraI DsaI EarI EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeIII
HapII HgaI Hin1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI PleI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI