##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AccI AccII AccIII AcyI
AgeI AhdI Aor51HI AscI AspEI
AspI AviII BamHI BbeI BbrPI
BcgI BciVI BclI BcnI BsaAI
BsaHI BseAI BsiEI BsiWI BsmBI
BspEI BssHII Bst1107I BstBI BstUI
BstZ17I CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoT22I FauI
FbaI FseI FspI HaeII Hin1I
HpaI KasI KspI MfeI MluI
MroI MunI MvnI NarI NciI
NheI NotI NruI NsiI NspV
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PvuI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
StuI SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 3427 GGTACC G^GTACC
Alw44I 4422 GTGCAC G^TGCAC
ApaI 3139 GGGCCC GGGCC^C
ApaLI 4422 GTGCAC G^TGCAC
Asp718I 3427 GGTACC G^GTACC
AvaI 3353 CTCGGG C^[CT]CG[GA]G
AvrII 4616 CCTAGG C^CTAGG
BalI 4454 TGGCCA TGG^CCA
BglI 2843 GCCAGAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 864 AGATCT A^GATCT
BlnI 4616 CCTAGG C^CTAGG
BsaWI 2183 ACCGGA [AT]^CCGG[AT]
BsgI 1084 GTGCAG GTGCAG(16/14)
BsoBI 3353 CTCGGG C^[CT]CG[GA]G
BspDI 5031 ATCGAT AT^CGAT
BsrBI 2608 GAGCGG CCGCTC(-3/-3)
BsrFI 1263 GCCGGC [GA]^CCGG[CT]
BstAPI 1032 GCAACCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2179 GGTGACC G^GT[ACGT]ACC
BstPI 2179 GGTGACC G^GT[ACGT]ACC
BstXI 1103 CCACCCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 742 GCGC GCG^C
Cfr10I 1263 GCCGGC [GA]^CCGG[CT]
ClaI 5031 ATCGAT AT^CGAT
EcoO65I 2179 GGTGACC G^GT[ACGT]ACC
EcoRI 5197 GAATTC G^AATTC
EcoRV 1767 GATATC GAT^ATC
HhaI 742 GCGC GCG^C
HinP1I 742 GCGC G^CGC
KpnI 3427 GGTACC GGTAC^C
MluNI 4454 TGGCCA TGG^CCA
MscI 4454 TGGCCA TGG^CCA
NaeI 1263 GCCGGC GCC^GGC
NdeI 3845 CATATG CA^TATG
NgoMIV 1263 GCCGGC G^CCGGC
PacI 4699 TTAATTAA TTAAT^TAA
SexAI 2327 ACCAGGT A^CC[AT]GGT
SpeI 202 ACTAGT A^CTAGT
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AclI 4713 AACGTT AA^CGTT
4827 AACGTT
AlwNI 548 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
3044 CAGGATCTG
BlpI 1861 GCTAAGC GC^T[ACGT]AGC
1981 GCTGAGC
BpmI 371 CTCCAG CTGGAG(16/14)
1965 CTGGAG
Bpu1102I 1861 GCTAAGC GC^T[ACGT]AGC
1981 GCTGAGC
BsaBI 25 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
121 GATGTTAATC
BsaI 324 GAGACC GGTCTC(1/5)
2919 GAGACC
BseRI 4222 CTCCTC GAGGAG(10/8)
964 GAGGAG
CelII 1861 GCTAAGC GC^T[ACGT]AGC
1981 GCTGAGC
DrdI 2604 GACAGAGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5147 GACAACTCTGTC
EaeI 3250 CGGCCA [CT]^GGCC[GA]
4454 TGGCCA
EcoNI 780 CCTAGATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3148 CCTTTCCCAGG
HgaI 3282 GCGTC GACGC(5/10)
3788 GCGTC
MamI 25 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
121 GATGTTAATC
Psp1406I 4713 AACGTT AA^CGTT
4827 AACGTT
SacI 1984 GAGCTC GAGCT^C
2431 GAGCTC
ScaI 680 AGTACT AGT^ACT
3413 AGTACT
XbaI 996 TCTAGA T^CTAGA
3508 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI AocI ApoI AseI
AsnI Asp700I AspHI AvaII BanI
BanII BbsI BbvCI BbvI BfaI
BfrI BmyI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspHI BspLU11I BspMI
BsrDI BsrGI BsrI BssKI BssSI
BstF5I BstNI BstYI Bsu36I Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EarI Eco81I EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PshBI
PstI PvuII RcaI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI