##################################
# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AvaI
AviII BalI BamHI BanI BbeI
BbrPI BbsI BcgI BclI BcnI
BfrI BglI BglII BpuAI BsaHI
BsaI BsaWI BseAI BsgI BsoBI
Bsp1407I BspEI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cfr10I CpoI DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV FauI
FbaI FseI FspI HapII HgaI
Hin1I HpaI HpaII KasI KpnI
KspI MluI MluNI MroI MscI
MspI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NspV PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI StuI SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AspHI 826 GAGCAC G[AT]GC[AT]^C
AvrII 270 CCTAGG C^CTAGG
BanII 1202 GAGCCC G[GA]GC[CT]^C
BbvCI 1041 GCTGAGG CCTCAGC(-5/-2)
BciVI 2468 GTATCC GTATCC(6/5)
BlnI 270 CCTAGG C^CTAGG
BlpI 1806 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1806 GCTAAGC GC^T[ACGT]AGC
BsaAI 966 TACGTG [CT]AC^GT[GA]
BsaBI 891 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 578 CGATCG CG[GA][CT]^CG
BsiHKAI 826 GAGCAC G[AT]GC[AT]^C
BsiWI 613 CGTACG C^GTACG
BsmBI 609 GAGACG CGTCTC(1/5)
BsmFI 1633 GTCCC GGGAC(10/14)
BspDI 264 ATCGAT AT^CGAT
BspLU11I 2561 ACATGT A^CATGT
BspMI 1679 ACCTGC ACCTGC(4/8)
BsrDI 445 CATTGC GCAATG(2/0)
BstAPI 1101 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 984 GGATCT [GA]^GATC[CT]
CelII 1806 GCTAAGC GC^T[ACGT]AGC
CfoI 1697 GCGC GCG^C
ClaI 264 ATCGAT AT^CGAT
DraII 1631 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 1489 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 1631 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 2387 GAATTC G^AATTC
HaeII 1696 AGCGCC [GA]GCGC^[CT]
HhaI 1697 GCGC GCG^C
HinP1I 1697 GCGC G^CGC
HindIII 538 AAGCTT A^AGCTT
MamI 891 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1405 CAATTG C^AATTG
MflI 984 GGATCT [GA]^GATC[CT]
MunI 1405 CAATTG C^AATTG
NdeI 975 CATATG CA^TATG
NlaIV 1632 GGTCCC GG[ACGT]^[ACGT]CC
PacI 89 TTAATTAA TTAAT^TAA
PflMI 1089 CCAATGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 436 GAGTC GAGTC(4/5)
PpuMI 1631 AGGTCCC [GA]G^G[AT]CC[CT]
PvuI 578 CGATCG CGAT^CG
SapI 1785 GAAGAGC GCTCTTC(1/4)
SpeI 112 ACTAGT A^CTAGT
Van91I 1089 CCAATGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 791 TCTAGA T^CTAGA
XcmI 331 CCAATATTTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 984 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 455 ACGTGT A^C[GA][CT]GT
2561 ACATGT
Asp700I 536 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2409 GAATTTTTTC
BmyI 826 GAGCAC G[AGT]GC[ACT]^C
1202 GAGCCC
Bsp1286I 826 GAGCAC G[AGT]GC[ACT]^C
1202 GAGCCC
BspHI 803 TCATGA T^CATGA
833 TCATGA
EcoT22I 1092 ATGCAT ATGCA^T
1928 ATGCAT
HaeIII 863 GGCC GG^CC
930 GGCC
HincII 438 GTCAAC GT[CT]^[GA]AC
1247 GTCAAC
HindII 438 GTCAAC GT[CT]^[GA]AC
1247 GTCAAC
MslI 975 CATATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1999 CATTCTAGTG
MspA1I 2177 CAGCTG C[AC]G^C[GT]G
2279 CAGCTG
NsiI 1092 ATGCAT ATGCA^T
1928 ATGCAT
NspI 131 GCATGT [GA]CATG^[CT]
2561 ACATGT
PvuII 2177 CAGCTG CAG^CTG
2279 CAGCTG
RcaI 803 TCATGA T^CATGA
833 TCATGA
SmlI 237 CTCAAG C^T[CT][GA]AG
2271 CTTGAG
Tsp45I 1169 GTCAC ^GT[GC]AC
1569 GTCAC
XmnI 536 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2409 GAATTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI AvaII
BbvI BfaI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraI EarI EcoRII
EcoT14I Fnu4HI FokI HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I