##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AgeI AhdI Aor51HI ApoI
AscI Asp718I AspEI AspI AviII
BbeI BbrPI BbsI BpuAI BsaAI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I Cfr10I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV FseI FspI HaeII KasI
KpnI KspI MfeI MroI MunI
NaeI NarI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AcyI 655 GGCGTC G[GA]^CG[CT]C
AflII 145 CTTAAG C^TTAAG
Alw44I 1232 GTGCAC G^TGCAC
ApaLI 1232 GTGCAC G^TGCAC
AseI 3304 ATTAAT AT^TAAT
AsnI 3304 ATTAAT AT^TAAT
AvrII 2543 CCTAGG C^CTAGG
BanI 1645 GGTGCC G^G[CT][GA]CC
BbvCI 239 GCTGAGG CCTCAGC(-5/-2)
BcgI 1422 CGAGGGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1412 GTATCC GTATCC(6/5)
BclI 2212 TGATCA T^GATCA
BfrI 145 CTTAAG C^TTAAG
BglII 1818 AGATCT A^GATCT
BlnI 2543 CCTAGG C^CTAGG
BsaBI 1819 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 655 GGCGTC G[GA]^CG[CT]C
BsmBI 567 GAGACG CGTCTC(1/5)
BsmI 2820 GCATTC GAATGC(1/-1)
BspMI 1608 ACCTGC ACCTGC(4/8)
BsrDI 1315 GCAATG GCAATG(2/0)
BstEII 2868 GGTTACC G^GT[ACGT]ACC
BstPI 2868 GGTTACC G^GT[ACGT]ACC
DrdI 3037 GACTCTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2868 GGTTACC G^GT[ACGT]ACC
EcoT22I 2097 ATGCAT ATGCA^T
FbaI 2212 TGATCA T^GATCA
Hin1I 655 GGCGTC G[GA]^CG[CT]C
HindIII 1161 AAGCTT A^AGCTT
MamI 1819 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 1911 CATATG CA^TATG
NheI 747 GCTAGC G^CTAGC
NsiI 2097 ATGCAT ATGCA^T
PshBI 3304 ATTAAT AT^TAAT
SapI 1472 GAAGAGC GCTCTTC(1/4)
TaqI 334 TCGA T^CGA
Tsp45I 384 GTGAC ^GT[GC]AC
TthHB8I 334 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 4 ACGCGT A^C[GA][CT]GT
1220 ACGCGT
AvaI 273 CCCGGG C^[CT]CG[GA]G
2826 CCCGGG
BamHI 58 GGATCC G^GATCC
586 GGATCC
BsoBI 273 CCCGGG C^[CT]CG[GA]G
2826 CCCGGG
BsrBI 10 CCGCTC CCGCTC(-3/-3)
3272 GAGCGG
BstAPI 489 GCACTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1530 GCACTTGCTGC
BstXI 590 CCACAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1906 CCAGGCATATGG
CfoI 350 GCGC GCG^C
651 GCGC
DraIII 490 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1771 CACACTGTG
HhaI 350 GCGC GCG^C
651 GCGC
HinP1I 350 GCGC G^CGC
651 GCGC
HpaI 1198 GTTAAC GTT^AAC
2017 GTTAAC
MaeII 2278 ACGT A^CGT
2298 ACGT
MluI 4 ACGCGT A^CGCGT
1220 ACGCGT
PstI 1483 CTGCAG CTGCA^G
2850 CTGCAG
SmaI 273 CCCGGG CCC^GGG
2826 CCCGGG
XcmI 1108 CCATTAGATGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1443 CCAGAAAGTGATTGG
XmaCI 273 CCCGGG C^CCGGG
2826 CCCGGG
XmaI 273 CCCGGG C^CCGGG
2826 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI Asp700I AspHI
AvaII BalI BanII BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CelII
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StyI
TfiI Tru9I TseI Tsp509I TspRI
Van91I XbaI XhoII XmnI