##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI BbrPI BcgI
BciVI BclI BfrI BsaAI BsaBI
BsaWI BseAI BsiWI BsmBI BsmI
BspDI BspEI BspHI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII Eam1105I Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KpnI MamI
MfeI MluI MroI MseI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI Tru9I Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 686 GTCTAC GT^[AC][GT]AC
AcyI 2223 GGCGCC G[GA]^CG[CT]C
AflIII 360 ACATGT A^C[GA][CT]GT
AviII 1082 TGCGCA TGC^GCA
AvrII 2710 CCTAGG C^CTAGG
BamHI 2965 GGATCC G^GATCC
BbeI 2223 GGCGCC GGCGC^C
BglII 216 AGATCT A^GATCT
BlnI 2710 CCTAGG C^CTAGG
BsaHI 2223 GGCGCC G[GA]^CG[CT]C
Bsp1407I 3460 TGTACA T^GTACA
BspLU11I 360 ACATGT A^CATGT
BspMI 1259 GCAGGT ACCTGC(4/8)
BsrGI 3460 TGTACA T^GTACA
BssSI 176 CTCGTG CACGAG(-5/-1)
BstAPI 398 GCAACTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 940 GACCCACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2242 CGGCCG C^GGCCG
EclXI 2242 CGGCCG C^GGCCG
Eco52I 2242 CGGCCG C^GGCCG
FspI 1082 TGCGCA TGC^GCA
HgaI 1672 GACGC GACGC(5/10)
Hin1I 2223 GGCGCC G[GA]^CG[CT]C
KasI 2223 GGCGCC G^GCGCC
KspI 115 CCGCGG CCGC^GG
NarI 2223 GGCGCC GG^CGCC
PflMI 2428 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 115 CCGCGG CCGC^GG
SfoI 2223 GGCGCC GGC^GCC
SmaI 40 CCCGGG CCC^GGG
SphI 2181 GCATGC GCATG^C
SspBI 3460 TGTACA T^GTACA
TaqI 282 TCGA T^CGA
TthHB8I 282 TCGA T^CGA
Van91I 2428 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1204 TCTAGA T^CTAGA
XmaCI 40 CCCGGG C^CCGGG
XmaI 40 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Asp700I 469 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2095 GAAGAAGTTC
AvaI 40 CCCGGG C^[CT]CG[GA]G
563 CCCGAG
BalI 534 TGGCCA TGG^CCA
2187 TGGCCA
BglI 38 GCCCCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2266 GCCTGACCGGC
BsaI 307 GGTCTC GGTCTC(1/5)
3126 GGTCTC
BsiEI 829 CGACCG CG[GA][CT]^CG
2242 CGGCCG
BsoBI 40 CCCGGG C^[CT]CG[GA]G
563 CCCGAG
BsrBI 1657 CCGCTC CCGCTC(-3/-3)
1411 GAGCGG
BsrDI 519 GCAATG GCAATG(2/0)
1590 GCAATG
BstYI 216 AGATCT [GA]^GATC[CT]
2965 GGATCC
HincII 1745 GTTGAC GT[CT]^[GA]AC
2675 GTTGAC
HindII 1745 GTTGAC GT[CT]^[GA]AC
2675 GTTGAC
MflI 216 AGATCT [GA]^GATC[CT]
2965 GGATCC
MluNI 534 TGGCCA TGG^CCA
2187 TGGCCA
MscI 534 TGGCCA TGG^CCA
2187 TGGCCA
ScaI 886 AGTACT AGT^ACT
1302 AGTACT
TfiI 934 GATTC G^A[AT]TC
3580 GAATC
XhoII 216 AGATCT [GA]^GATC[CT]
2965 GGATCC
XmnI 469 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2095 GAAGAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaII
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TseI Tsp45I Tsp509I
TspRI XcmI