##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbrPI BcgI BciVI BclI
BfrI BglII BlnI BsaAI BsaBI
BsaWI BseAI BsiWI BspDI BspEI
BspLU11I BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI DraIII Eam1105I
Eco47III EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI MamI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SapI SbfI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspI
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 569 GACGTC GACGT^C
AcsI 544 GAATTC [GA]^AATT[CT]
Alw44I 2314 GTGCAC G^TGCAC
AocI 3020 CCTGAGG CC^T[ACGT]AGG
ApaLI 2314 GTGCAC G^TGCAC
ApoI 544 GAATTC [GA]^AATT[CT]
BamHI 1910 GGATCC G^GATCC
BbeI 1120 GGCGCC GGCGC^C
BbvCI 2217 CCTCAGC CCTCAGC(-5/-2)
BsmI 1858 GAATGC GAATGC(1/-1)
Bsp1407I 1086 TGTACA T^GTACA
BspHI 1323 TCATGA T^CATGA
BsrGI 1086 TGTACA T^GTACA
Bsu36I 3020 CCTGAGG CC^T[ACGT]AGG
DrdI 148 GACCCAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1006 CGGCCG C^GGCCG
EclXI 1006 CGGCCG C^GGCCG
Eco52I 1006 CGGCCG C^GGCCG
Eco81I 3020 CCTGAGG CC^T[ACGT]AGG
EcoRI 544 GAATTC G^AATTC
HincII 1277 GTCGAC GT[CT]^[GA]AC
HindII 1277 GTCGAC GT[CT]^[GA]AC
KasI 1120 GGCGCC G^GCGCC
MseI 525 TTAA T^TAA
NarI 1120 GGCGCC GG^CGCC
PflMI 1724 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1323 TCATGA T^CATGA
SalI 1277 GTCGAC G^TCGAC
ScaI 843 AGTACT AGT^ACT
SexAI 699 ACCTGGT A^CC[AT]GGT
SfiI 2732 GGCCGGCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1120 GGCGCC GGC^GCC
SspBI 1086 TGTACA T^GTACA
TaqI 1278 TCGA T^CGA
TfiI 300 GAATC G^A[AT]TC
Tru9I 525 TTAA T^TAA
Tsp509I 545 AATT ^AATT
TthHB8I 1278 TCGA T^CGA
Van91I 1724 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2045 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1277 GTCGAC GT^[AC][GT]AC
1333 GTCTAC
Asp700I 1600 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2478 GAAGTCCTTC
BalI 588 TGGCCA TGG^CCA
2804 TGGCCA
BfaI 1393 CTAG C^TAG
2046 CTAG
BglI 102 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2733 GCCGGCAAGGC
BsiEI 1006 CGGCCG CG[GA][CT]^CG
1156 CGACCG
BsrBI 361 CCGCTC CCGCTC(-3/-3)
1431 GAGCGG
BsrDI 1177 CATTGC GCAATG(2/0)
2911 CATTGC
BsrFI 2600 GCCGGC [GA]^CCGG[CT]
2733 GCCGGC
BssSI 2533 CACGAG CACGAG(-5/-1)
584 CTCGTG
BstAPI 265 GCAACGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1365 GCAAGACCTGC
BstEII 502 GGTCACC G^GT[ACGT]ACC
2773 GGTCACC
BstPI 502 GGTCACC G^GT[ACGT]ACC
2773 GGTCACC
BstYI 1023 AGATCC [GA]^GATC[CT]
1910 GGATCC
Cfr10I 2600 GCCGGC [GA]^CCGG[CT]
2733 GCCGGC
EcoO65I 502 GGTCACC G^GT[ACGT]ACC
2773 GGTCACC
KspI 260 CCGCGG CCGC^GG
2287 CCGCGG
MaeI 1393 CTAG C^TAG
2046 CTAG
MflI 1023 AGATCC [GA]^GATC[CT]
1910 GGATCC
MluNI 588 TGGCCA TGG^CCA
2804 TGGCCA
MscI 588 TGGCCA TGG^CCA
2804 TGGCCA
NaeI 2600 GCCGGC GCC^GGC
2733 GCCGGC
NgoMIV 2600 GCCGGC G^CCGGC
2733 GCCGGC
PshAI 1550 GACGGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2767 GACAACGGTC
SacI 181 GAGCTC GAGCT^C
324 GAGCTC
SacII 260 CCGCGG CCGC^GG
2287 CCGCGG
SphI 801 GCATGC GCATG^C
853 GCATGC
XhoII 1023 AGATCC [GA]^GATC[CT]
1910 GGATCC
XmnI 1600 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2478 GAAGTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StuI StyI
TseI Tsp45I TspRI XcmI XmaCI
XmaI