##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AspEI AspHI AspI
AvaI AviII BamHI BanII BbeI
BbrPI BcgI BciVI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsoBI BspDI
BspEI BsrFI BssHII BssSI BstAPI
Bsu36I Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoRI
FseI FspI HaeII HgaI Hin1I
HincII HindII HpaI KasI Ksp632I
KspI MamI MfeI MluI MroI
MunI NaeI NarI NcoI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 1617 GGTACC G^GTACC
AflII 599 CTTAAG C^TTAAG
AlwNI 394 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 1617 GGTACC G^GTACC
BbvCI 848 CCTCAGC CCTCAGC(-5/-2)
BclI 2975 TGATCA T^GATCA
BcnI 33 CCGGG CC^[GC]GG
BfrI 599 CTTAAG C^TTAAG
BglII 124 AGATCT A^GATCT
BlpI 3282 GCTGAGC GC^T[ACGT]AGC
BmyI 3793 GTGCCC G[AGT]GC[ACT]^C
BpmI 265 CTGGAG CTGGAG(16/14)
Bpu1102I 3282 GCTGAGC GC^T[ACGT]AGC
BseRI 1794 CTCCTC GAGGAG(10/8)
Bsp1286I 3793 GTGCCC G[AGT]GC[ACT]^C
Bsp1407I 2087 TGTACA T^GTACA
BspHI 2610 TCATGA T^CATGA
BsrBI 3285 GAGCGG CCGCTC(-3/-3)
BsrGI 2087 TGTACA T^GTACA
BstBI 3520 TTCGAA TT^CGAA
BstEII 3988 GGTAACC G^GT[ACGT]ACC
BstPI 3988 GGTAACC G^GT[ACGT]ACC
BstXI 2232 CCAACTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 3282 GCTGAGC GC^T[ACGT]AGC
EcoNI 1041 CCTTTCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3988 GGTAACC G^GT[ACGT]ACC
EcoRV 4147 GATATC GAT^ATC
FauI 13 GCGGG CCCGC(4/6)
FbaI 2975 TGATCA T^GATCA
HapII 33 CCGG C^CGG
HpaII 33 CCGG C^CGG
KpnI 1617 GGTACC GGTAC^C
MspI 33 CCGG C^CGG
NciI 33 CCGGG CC^[GC]GG
NspV 3520 TTCGAA TT^CGAA
PpuMI 2044 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 84 CAGCTG CAG^CTG
RcaI 2610 TCATGA T^CATGA
SfuI 3520 TTCGAA TT^CGAA
SphI 1015 GCATGC GCATG^C
SspBI 2087 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 1 CGCG CG^CG
12 CGCG
AlwI 2361 GATCC GGATC(4/5)
2883 GGATC
AvaII 674 GGACC G^G[AT]CC
2045 GGACC
AvrII 928 CCTAGG C^CTAGG
2870 CCTAGG
BanI 1617 GGTACC G^G[CT][GA]CC
3456 GGTGCC
BglI 19 GCCTGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
415 GCCCTGAAGGC
BlnI 928 CCTAGG C^CTAGG
2870 CCTAGG
BsaI 3920 GAGACC GGTCTC(1/5)
1325 GGTCTC
Bst1107I 1471 GTATAC GTA^TAC
2600 GTATAC
BstNI 20 CCTGG CC^[AT]GG
1976 CCTGG
BstUI 1 CGCG CG^CG
12 CGCG
BstZ17I 1471 GTATAC GTA^TAC
2600 GTATAC
DraII 413 AGGCCCT [GA]G^G[ACGT]CC[CT]
2044 AGGACCT
EcoO109I 413 AGGCCCT [GA]G^G[ACGT]CC[CT]
2044 AGGACCT
EcoRII 20 CCTGG ^CC[AT]GG
1976 CCTGG
EcoT22I 939 ATGCAT ATGCA^T
2843 ATGCAT
HindIII 1873 AAGCTT A^AGCTT
3774 AAGCTT
MspA1I 70 CCGCTG C[AC]G^C[GT]G
84 CAGCTG
MvaI 20 CCTGG CC^[AT]GG
1976 CCTGG
MvnI 1 CGCG CG^CG
12 CGCG
NdeI 1451 CATATG CA^TATG
3857 CATATG
NsiI 939 ATGCAT ATGCA^T
2843 ATGCAT
PflMI 1410 CCAACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3924 CCAAAACGTGG
SspI 114 AATATT AAT^ATT
1482 AATATT
TaqI 324 TCGA T^CGA
3521 TCGA
TthHB8I 324 TCGA T^CGA
3521 TCGA
Van91I 1410 CCAACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3924 CCAAAACGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI ApoI AseI AsnI Asp700I
BalI BbsI BbvI BfaI BpuAI
BsaJI BsiYI BslI BsmAI BsmFI
BsmI BspLU11I BspMI BsrDI BsrI
BssKI BstF5I BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI EaeI
EcoT14I Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MwoI NdeII NlaIII NlaIV NspI
PleI PshBI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XhoII XmnI