##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AocI ApoI AscI AseI
AsnI Asp718I AspI AvrII BamHI
BciVI BclI BfrI BlnI BsaBI
BsiWI BsmBI Bsp1407I BspDI BspLU11I
BsrBI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
EarI Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI HincII HindII HindIII
HpaI KpnI Ksp632I MamI MfeI
MunI NdeI NheI NotI NruI
NsiI NspI NspV PacI PflFI
PmeI PshBI Psp1406I PvuI RsrII
SalI SapI SexAI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI Tsp509I Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 1725 TCCGGA T^CCGGA
AgeI 387 ACCGGT A^CCGGT
Alw44I 2873 GTGCAC G^TGCAC
ApaI 2374 GGGCCC GGGCC^C
ApaLI 2873 GTGCAC G^TGCAC
Asp700I 997 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 185 TGCGCA TGC^GCA
BbeI 1816 GGCGCC GGCGC^C
BcgI 157 CGAGGAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 828 AGATCT A^GATCT
BseAI 1725 TCCGGA T^CCGGA
BsmI 2547 GCATTC GAATGC(1/-1)
BspEI 1725 TCCGGA T^CCGGA
DrdI 689 GACTACACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1267 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 185 TGCGCA TGC^GCA
KasI 1816 GGCGCC G^GCGCC
KspI 242 CCGCGG CCGC^GG
MluI 4 ACGCGT A^CGCGT
MroI 1725 TCCGGA T^CCGGA
NarI 1816 GGCGCC GG^CGCC
PinAI 387 ACCGGT A^CCGGT
PshAI 138 GACCACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 242 CCGCGG CCGC^GG
SfiI 1279 GGCCGAGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1816 GGCGCC GGC^GCC
XmnI 997 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 1816 GGCGCC G[GA]^CG[CT]C
1854 GACGCC
AflIII 4 ACGCGT A^C[GA][CT]GT
534 ACGTGT
AhdI 1949 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2119 GACAACAGGTC
AlwI 274 GATCC GGATC(4/5)
916 GATCC
AspEI 1949 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2119 GACAACAGGTC
BbrPI 314 CACGTG CAC^GTG
533 CACGTG
BbsI 2787 GAAGAC GAAGAC(2/6)
2683 GTCTTC
BglI 1280 GCCGAGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2324 GCCCCGCTGGC
BpuAI 2787 GAAGAC GAAGAC(2/6)
2683 GTCTTC
BsaHI 1816 GGCGCC G[GA]^CG[CT]C
1854 GACGCC
BsgI 383 CTGCAC GTGCAG(16/14)
1256 GTGCAG
BstYI 828 AGATCT [GA]^GATC[CT]
915 AGATCC
EagI 1278 CGGCCG C^GGCCG
1287 CGGCCG
Eam1105I 1949 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2119 GACAACAGGTC
EclXI 1278 CGGCCG C^GGCCG
1287 CGGCCG
Eco52I 1278 CGGCCG C^GGCCG
1287 CGGCCG
FseI 936 GGCCGGCC GGCCGG^CC
1288 GGCCGGCC
Hin1I 1816 GGCGCC G[GA]^CG[CT]C
1854 GACGCC
MaeIII 1362 GTGAC ^GT[ACGT]AC
2117 GTGAC
MflI 828 AGATCT [GA]^GATC[CT]
915 AGATCC
MseI 2852 TTAA T^TAA
2915 TTAA
NcoI 2036 CCATGG C^CATGG
2523 CCATGG
PaeR7I 843 CTCGAG C^TCGAG
1501 CTCGAG
PmaCI 314 CACGTG CAC^GTG
533 CACGTG
PmlI 314 CACGTG CAC^GTG
533 CACGTG
SbfI 1466 CCTGCAGG CCTGCA^GG
1579 CCTGCAGG
ScaI 870 AGTACT AGT^ACT
900 AGTACT
Sse8387I 1466 CCTGCAGG CCTGCA^GG
1579 CCTGCAGG
StuI 468 AGGCCT AGG^CCT
2847 AGGCCT
Tru9I 2852 TTAA T^TAA
2915 TTAA
Tsp45I 1362 GTGAC ^GT[GC]AC
2117 GTGAC
XhoI 843 CTCGAG C^TCGAG
1501 CTCGAG
XhoII 828 AGATCT [GA]^GATC[CT]
915 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
Aor51HI AspHI AvaI AvaII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaAI BsaI BsaJI BsaWI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI Eco47III EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI TseI TspRI TthHB8I
Van91I XcmI XmaCI XmaI