##################################
# NO CUT ENZYMES ( 36 enzymes) #
##################################
AclI AgeI AscI AseI AsnI
BbeI BsiWI Bst1107I BstBI BstZ17I
CpoI EcoRI EcoT22I FseI KasI
KspI NarI NotI NruI NsiI
NspV PacI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SfiI
SfoI SfuI SgrAI SnaBI SwaI
XbaI
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# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 6010 GACGTC GACGT^C
Acc65I 42 GGTACC G^GTACC
AccIII 7640 TCCGGA T^CCGGA
AflII 8308 CTTAAG C^TTAAG
Asp700I 5892 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 42 GGTACC G^GTACC
AviII 5969 TGCGCA TGC^GCA
AvrII 8262 CCTAGG C^CTAGG
BcgI 1383 CGACGCTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1991 TGATCA T^GATCA
BfrI 8308 CTTAAG C^TTAAG
BglII 2300 AGATCT A^GATCT
BlnI 8262 CCTAGG C^CTAGG
BsaBI 10157 GATACTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 7640 TCCGGA T^CCGGA
BspDI 2071 ATCGAT AT^CGAT
BspEI 7640 TCCGGA T^CCGGA
BssHII 3131 GCGCGC G^CGCGC
ClaI 2071 ATCGAT AT^CGAT
FbaI 1991 TGATCA T^GATCA
FspI 5969 TGCGCA TGC^GCA
HindIII 7654 AAGCTT A^AGCTT
HpaI 5113 GTTAAC GTT^AAC
KpnI 42 GGTACC GGTAC^C
MamI 10157 GATACTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 7584 CAATTG C^AATTG
MluI 4574 ACGCGT A^CGCGT
MroI 7640 TCCGGA T^CCGGA
MunI 7584 CAATTG C^AATTG
NdeI 2465 CATATG CA^TATG
SalI 434 GTCGAC G^TCGAC
SpeI 10251 ACTAGT A^CTAGT
SspI 760 AATATT AAT^ATT
XmnI 5892 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 434 GTCGAC GT^[AC][GT]AC
2737 GTAGAC
AhdI 2122 GACCAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
8681 GACGAAACGTC
Alw44I 1093 GTGCAC G^TGCAC
6097 GTGCAC
Aor51HI 998 AGCGCT AGC^GCT
3209 AGCGCT
ApaLI 1093 GTGCAC G^TGCAC
6097 GTGCAC
AspEI 2122 GACCAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
8681 GACGAAACGTC
BamHI 5124 GGATCC G^GATCC
7893 GGATCC
BciVI 1792 GTATCC GTATCC(6/5)
6998 GTATCC
BsiEI 1966 CGGCCG CG[GA][CT]^CG
7778 CGGCCG
BssSI 5723 CACGAG CACGAG(-5/-1)
6052 CACGAG
DraI 8846 TTTAAA TTT^AAA
10122 TTTAAA
DraIII 3112 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
3280 CACGTGGTG
DrdI 6688 GACAAACCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7695 GACCACCAAGTC
EagI 1966 CGGCCG C^GGCCG
7778 CGGCCG
Eam1105I 2122 GACCAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
8681 GACGAAACGTC
EclXI 1966 CGGCCG C^GGCCG
7778 CGGCCG
Eco47III 998 AGCGCT AGC^GCT
3209 AGCGCT
Eco52I 1966 CGGCCG C^GGCCG
7778 CGGCCG
EcoRV 1282 GATATC GAT^ATC
2956 GATATC
SacI 1154 GAGCTC GAGCT^C
1932 GAGCTC
SbfI 3405 CCTGCAGG CCTGCA^GG
4437 CCTGCAGG
ScaI 1044 AGTACT AGT^ACT
8119 AGTACT
SphI 587 GCATGC GCATG^C
4427 GCATGC
Sse8387I 3405 CCTGCAGG CCTGCA^GG
4437 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 164 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AspI AvaI
AvaII BalI BanI BanII BbrPI
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NheI NlaIII NlaIV NspI
PaeR7I PflFI PflMI PleI PmaCI
PmlI PpuMI PshAI PstI PvuII
RcaI RsaI SapI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmaI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoI XhoII XmaCI XmaI