##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccI AclI AcyI Alw44I
ApaLI AscI AseI AsnI AspI
AviII BbeI BbrPI BcgI BsaHI
BsiWI Bsp1407I BspDI BsrGI Bst1107I
BstBI BstZ17I ClaI CpoI DraIII
EagI EclXI Eco52I FseI FspI
Hin1I KasI KspI MluI NarI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SmaI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AflII 1346 CTTAAG C^TTAAG
AgeI 936 ACCGGT A^CCGGT
AhdI 4064 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2685 AGCGCT AGC^GCT
AspEI 4064 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcnI 402 CCGGG CC^[GC]GG
BfrI 1346 CTTAAG C^TTAAG
BlpI 4744 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4744 GCTGAGC GC^T[ACGT]AGC
BsaBI 3879 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 648 CGGTCG CG[GA][CT]^CG
BsmBI 4089 CGTCTC CGTCTC(1/5)
BspHI 4418 TCATGA T^CATGA
BspLU11I 4906 ACATGT A^CATGT
BssHII 3 GCGCGC G^CGCGC
BssSI 4922 CACGAG CACGAG(-5/-1)
CelII 4744 GCTGAGC GC^T[ACGT]AGC
Eam1105I 4064 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2685 AGCGCT AGC^GCT
HgaI 39 GCGTC GACGC(5/10)
HincII 1649 GTTAAC GT[CT]^[GA]AC
HindII 1649 GTTAAC GT[CT]^[GA]AC
HpaI 1649 GTTAAC GTT^AAC
MamI 3879 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 3857 GCCGGC GCC^GGC
NciI 402 CCGGG CC^[GC]GG
NgoMIV 3857 GCCGGC G^CCGGC
PinAI 936 ACCGGT A^CCGGT
RcaI 4418 TCATGA T^CATGA
SacI 2881 GAGCTC GAGCT^C
ScaI 4537 AGTACT AGT^ACT
SexAI 2255 ACCTGGT A^CC[AT]GGT
SgrAI 935 CACCGGTG C[GA]^CCGG[CT]G
SspI 4487 AATATT AAT^ATT
TaqI 3645 TCGA T^CGA
TthHB8I 3645 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 2 CGCG CG^CG
4 CGCG
AccIII 56 TCCGGA T^CCGGA
6621 TCCGGA
AflIII 3160 ACGTGT A^C[GA][CT]GT
4906 ACATGT
AspHI 2881 GAGCTC G[AT]GC[AT]^C
3718 GTGCTC
BciVI 544 GTATCC GTATCC(6/5)
3364 GTATCC
BclI 6586 TGATCA T^GATCA
6741 TGATCA
BglI 2927 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5646 GCCAAGGGGGC
BglII 3998 AGATCT A^GATCT
5150 AGATCT
BseAI 56 TCCGGA T^CCGGA
6621 TCCGGA
BsgI 4965 CTGCAC GTGCAG(16/14)
164 GTGCAG
BsiHKAI 2881 GAGCTC G[AT]GC[AT]^C
3718 GTGCTC
BsmI 307 GCATTC GAATGC(1/-1)
666 GCATTC
BspEI 56 TCCGGA T^CCGGA
6621 TCCGGA
BsrBI 1566 GAGCGG CCGCTC(-3/-3)
3940 GAGCGG
BstUI 2 CGCG CG^CG
4 CGCG
DrdI 1880 GACTCACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2620 GACGAGGAGGTC
EcoRI 4269 GAATTC G^AATTC
5415 GAATTC
EcoRV 5834 GATATC GAT^ATC
5970 GATATC
EcoT22I 1012 ATGCAT ATGCA^T
4398 ATGCAT
FbaI 6586 TGATCA T^GATCA
6741 TGATCA
HaeII 2246 AGCGCC [GA]GCGC^[CT]
2685 AGCGCT
HindIII 3902 AAGCTT A^AGCTT
5518 AAGCTT
MfeI 3303 CAATTG C^AATTG
5426 CAATTG
MroI 56 TCCGGA T^CCGGA
6621 TCCGGA
MunI 3303 CAATTG C^AATTG
5426 CAATTG
MvnI 2 CGCG CG^CG
4 CGCG
NsiI 1012 ATGCAT ATGCA^T
4398 ATGCAT
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
Asp700I Asp718I AvaI AvaII AvrII
BalI BamHI BanI BanII BbsI
BbvCI BbvI BfaI BlnI BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BsaWI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PshAI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XbaI XcmI XhoII XmnI