##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BciVI
BfrI BglI BlnI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspLU11I
BsrBI BsrDI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoT22I FseI FspI HaeII Hin1I
HincII HindII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MspA1I MunI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
StuI SwaI TaqI TthHB8I XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccII 778 CGCG CG^CG
AhdI 94 GACTCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 252 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 516 CCTCAGG CC^T[ACGT]AGG
AspEI 94 GACTCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 202 GAGCAC G[AT]GC[AT]^C
AspI 894 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 750 CTCGGG C^[CT]CG[GA]G
BanI 618 GGTGCC G^G[CT][GA]CC
BbsI 1353 GAAGAC GAAGAC(2/6)
BclI 373 TGATCA T^GATCA
BglII 1425 AGATCT A^GATCT
BlpI 800 GCTCAGC GC^T[ACGT]AGC
BpmI 1324 CTGGAG CTGGAG(16/14)
Bpu1102I 800 GCTCAGC GC^T[ACGT]AGC
BpuAI 1353 GAAGAC GAAGAC(2/6)
BsgI 1576 CTGCAC GTGCAG(16/14)
BsiHKAI 202 GAGCAC G[AT]GC[AT]^C
BsmI 740 GAATGC GAATGC(1/-1)
BsoBI 750 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1942 TGTACA T^GTACA
BspHI 31 TCATGA T^CATGA
BsrFI 557 GCCGGT [GA]^CCGG[CT]
BsrGI 1942 TGTACA T^GTACA
BssSI 1514 CTCGTG CACGAG(-5/-1)
BstUI 778 CGCG CG^CG
BstXI 1775 CCAAGCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 516 CCTCAGG CC^T[ACGT]AGG
CelII 800 GCTCAGC GC^T[ACGT]AGC
Cfr10I 557 GCCGGT [GA]^CCGG[CT]
EaeI 920 TGGCCG [CT]^GGCC[GA]
Eam1105I 94 GACTCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 516 CCTCAGG CC^T[ACGT]AGG
EcoRV 567 GATATC GAT^ATC
FauI 322 CCCGC CCCGC(4/6)
FbaI 373 TGATCA T^GATCA
HindIII 39 AAGCTT A^AGCTT
MslI 2003 CATAAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 778 CGCG CG^CG
NcoI 1142 CCATGG C^CATGG
NdeI 603 CATATG CA^TATG
PflFI 894 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 31 TCATGA T^CATGA
ScaI 715 AGTACT AGT^ACT
SmlI 1818 CTCAAG C^T[CT][GA]AG
SphI 1229 GCATGC GCATG^C
SspBI 1942 TGTACA T^GTACA
Tth111I 894 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1387 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 1534 AAATTT [GA]^AATT[CT]
2014 GAATTT
AfaI 716 GTAC GT^AC
1943 GTAC
ApaI 925 GGGCCC GGGCC^C
1202 GGGCCC
ApoI 1534 AAATTT [GA]^AATT[CT]
2014 GAATTT
BcgI 1506 GCAGCCTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1578 GCACTGTGATCG
BcnI 433 CCCGG CC^[GC]GG
923 CCGGG
BsmAI 499 GAGAC GTCTC(1/5)
1717 GTCTC
CfoI 744 GCGC GCG^C
1431 GCGC
DsaI 577 CCGTGG C^C[GA][CT]GG
1142 CCATGG
EarI 507 GAAGAG CTCTTC(1/4)
1994 GAAGAG
HhaI 744 GCGC GCG^C
1431 GCGC
HinP1I 744 GCGC G^CGC
1431 GCGC
Ksp632I 507 GAAGAG CTCTTC(1/4)
1994 GAAGAG
NciI 433 CCCGG CC^[GC]GG
923 CCGGG
RsaI 716 GTAC GT^AC
1943 GTAC
SfaNI 1047 GCATC GCATC(5/9)
1086 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvI BfaI BmyI BsaJI BseRI
BsiYI BslI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI Sau3AI Sau96I ScrFI SfcI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XhoII