##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccIII AclI AscI AseI
AsnI Asp700I AspI AvrII BamHI
BbrPI BbvCI BcgI BlnI BsaAI
BsaBI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspEI BspHI BsrBI BsrGI
BssHII Bst1107I BstBI BstZ17I CpoI
DraI DraIII EagI EclXI Eco52I
EcoRI EcoRV FseI HpaI MamI
MluI MroI NaeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SnaBI SphI Sse8387I SspBI
SwaI Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 3338 GGTACC G^GTACC
AccI 4133 GTAGAC GT^[AC][GT]AC
AflII 2675 CTTAAG C^TTAAG
AflIII 4200 ACATGT A^C[GA][CT]GT
AgeI 132 ACCGGT A^CCGGT
AocI 2271 CCTAAGG CC^T[ACGT]AGG
Aor51HI 1510 AGCGCT AGC^GCT
Asp718I 3338 GGTACC G^GTACC
AviII 1034 TGCGCA TGC^GCA
BciVI 1561 GGATAC GTATCC(6/5)
BclI 2608 TGATCA T^GATCA
BfrI 2675 CTTAAG C^TTAAG
BspDI 1111 ATCGAT AT^CGAT
BspLU11I 4200 ACATGT A^CATGT
BssSI 835 CACGAG CACGAG(-5/-1)
BstEII 3726 GGTTACC G^GT[ACGT]ACC
BstPI 3726 GGTTACC G^GT[ACGT]ACC
Bsu36I 2271 CCTAAGG CC^T[ACGT]AGG
ClaI 1111 ATCGAT AT^CGAT
Eco47III 1510 AGCGCT AGC^GCT
Eco81I 2271 CCTAAGG CC^T[ACGT]AGG
EcoNI 1323 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3726 GGTTACC G^GT[ACGT]ACC
FbaI 2608 TGATCA T^GATCA
FspI 1034 TGCGCA TGC^GCA
HincII 1153 GTCAAC GT[CT]^[GA]AC
HindII 1153 GTCAAC GT[CT]^[GA]AC
KpnI 3338 GGTACC GGTAC^C
MfeI 2078 CAATTG C^AATTG
MunI 2078 CAATTG C^AATTG
NcoI 140 CCATGG C^CATGG
NdeI 3185 CATATG CA^TATG
PinAI 132 ACCGGT A^CCGGT
ScaI 1244 AGTACT AGT^ACT
SmaI 740 CCCGGG CCC^GGG
SpeI 4081 ACTAGT A^CTAGT
SspI 3806 AATATT AAT^ATT
StuI 1682 AGGCCT AGG^CCT
XbaI 3078 TCTAGA T^CTAGA
XmaCI 740 CCCGGG C^CCGGG
XmaI 740 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AhdI 1227 GACCACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3652 GACTGTATGTC
Alw44I 1318 GTGCAC G^TGCAC
1632 GTGCAC
ApaI 71 GGGCCC GGGCC^C
745 GGGCCC
ApaLI 1318 GTGCAC G^TGCAC
1632 GTGCAC
AspEI 1227 GACCACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3652 GACTGTATGTC
AvaI 740 CCCGGG C^[CT]CG[GA]G
1673 CTCGGG
BbeI 660 GGCGCC GGCGC^C
2290 GGCGCC
BglI 738 GCCCCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2282 GCCGTGGAGGC
BsaWI 132 ACCGGT [AT]^CCGG[AT]
2009 ACCGGA
BsmI 3388 GCATTC GAATGC(1/-1)
3513 GCATTC
BsoBI 740 CCCGGG C^[CT]CG[GA]G
1673 CTCGGG
BsrDI 867 GCAATG GCAATG(2/0)
1990 GCAATG
BstAPI 99 GCACCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
854 GCATGTACTGC
BstXI 52 CCAGGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2928 CCAGAACCCTGG
DrdI 68 GACGGGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
261 GACCCCAGAGTC
Eam1105I 1227 GACCACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3652 GACTGTATGTC
EcoT22I 3690 ATGCAT ATGCA^T
4231 ATGCAT
KasI 660 GGCGCC G^GCGCC
2290 GGCGCC
NarI 660 GGCGCC GG^CGCC
2290 GGCGCC
NsiI 3690 ATGCAT ATGCA^T
4231 ATGCAT
PshAI 1381 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1719 GACCCTGGTC
SacI 1885 GAGCTC GAGCT^C
2337 GAGCTC
SfoI 660 GGCGCC GGC^GCC
2290 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII