##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
Asp718I BciVI BclI BfrI BglII
BsiWI BsmI BspDI BspHI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
EcoRI EcoRV EcoT22I FbaI HindIII
HpaI KpnI KspI MfeI MluI
MunI NdeI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SapI SbfI ScaI SfuI
SgrAI SnaBI Sse8387I SspI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccI 5340 GTCGAC GT^[AC][GT]AC
AccIII 5787 TCCGGA T^CCGGA
AhdI 3070 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 6646 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3070 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1993 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2454 GGATCC G^GATCC
BbrPI 2559 CACGTG CAC^GTG
BseAI 5787 TCCGGA T^CCGGA
Bsp1407I 6577 TGTACA T^GTACA
BspEI 5787 TCCGGA T^CCGGA
BsrGI 6577 TGTACA T^GTACA
BstEII 1167 GGTGACC G^GT[ACGT]ACC
BstPI 1167 GGTGACC G^GT[ACGT]ACC
DraI 5221 TTTAAA TTT^AAA
Eam1105I 3070 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1167 GGTGACC G^GT[ACGT]ACC
FseI 3688 GGCCGGCC GGCCGG^CC
HincII 5340 GTCGAC GT[CT]^[GA]AC
HindII 5340 GTCGAC GT[CT]^[GA]AC
MroI 5787 TCCGGA T^CCGGA
NheI 6336 GCTAGC G^CTAGC
PflFI 1993 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2559 CACGTG CAC^GTG
PmlI 2559 CACGTG CAC^GTG
PshAI 5762 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 5340 GTCGAC G^TCGAC
SexAI 1994 ACCAGGT A^CC[AT]GGT
SpeI 3775 ACTAGT A^CTAGT
SspBI 6577 TGTACA T^GTACA
Tth111I 1993 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 6646 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 4809 GAATTT [GA]^AATT[CT]
6646 GAATTT
Aor51HI 70 AGCGCT AGC^GCT
1478 AGCGCT
ApoI 4809 GAATTT [GA]^AATT[CT]
6646 GAATTT
AviII 440 TGCGCA TGC^GCA
2312 TGCGCA
AvrII 50 CCTAGG C^CTAGG
2691 CCTAGG
BcgI 1196 CGAGCCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2796 CGACGGCCCTGC
BlnI 50 CCTAGG C^CTAGG
2691 CCTAGG
BsaAI 2559 CACGTG [CT]AC^GT[GA]
6250 TACGTG
BsaBI 1068 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4494 GATGAAGATC
BspLU11I 509 ACATGT A^CATGT
4999 ACATGT
BssSI 21 CACGAG CACGAG(-5/-1)
961 CACGAG
BstAPI 3790 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4084 GCAGAGATTGC
DrdI 1249 GACCTGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5343 GACTGTGGGGTC
Eco47III 70 AGCGCT AGC^GCT
1478 AGCGCT
FspI 440 TGCGCA TGC^GCA
2312 TGCGCA
MamI 1068 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4494 GATGAAGATC
MseI 5222 TTAA T^TAA
6468 TTAA
TfiI 502 GATTC G^A[AT]TC
5901 GATTC
Tru9I 5222 TTAA T^TAA
6468 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SfoI SmaI
SmlI SphI StuI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI