##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaLI
AscI Asp700I Asp718I AspEI AspI
AviII BbeI BbvCI BcgI BglI
BsaHI BsiEI BsiWI BspDI BspLU11I
BsrBI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I FseI FspI
HaeII Hin1I KasI KpnI KspI
MluI MvnI NarI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmeI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SphI Sse8387I
Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AflII 4937 CTTAAG C^TTAAG
AflIII 5144 ACGTGT A^C[GA][CT]GT
BamHI 1514 GGATCC G^GATCC
BbrPI 5143 CACGTG CAC^GTG
BclI 3402 TGATCA T^GATCA
BfrI 4937 CTTAAG C^TTAAG
BglII 729 AGATCT A^GATCT
BlpI 544 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 544 GCTGAGC GC^T[ACGT]AGC
BsaBI 253 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1716 GTGCAG GTGCAG(16/14)
BsrFI 37 GCCGGC [GA]^CCGG[CT]
CelII 544 GCTGAGC GC^T[ACGT]AGC
CfoI 2826 GCGC GCG^C
Cfr10I 37 GCCGGC [GA]^CCGG[CT]
DraIII 3639 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 4948 GAATTC G^AATTC
EcoRV 4477 GATATC GAT^ATC
EcoT22I 4499 ATGCAT ATGCA^T
FauI 11 GCGGG CCCGC(4/6)
FbaI 3402 TGATCA T^GATCA
HgaI 3961 GCGTC GACGC(5/10)
HhaI 2826 GCGC GCG^C
HinP1I 2826 GCGC G^CGC
HincII 392 GTTAAC GT[CT]^[GA]AC
HindII 392 GTTAAC GT[CT]^[GA]AC
HpaI 392 GTTAAC GTT^AAC
MamI 253 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 37 GCCGGC GCC^GGC
NgoMIV 37 GCCGGC G^CCGGC
NsiI 4499 ATGCAT ATGCA^T
PflMI 5109 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 5143 CACGTG CAC^GTG
PmlI 5143 CACGTG CAC^GTG
PpuMI 4327 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 4676 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1088 GAGCTC GAGCT^C
SmaI 50 CCCGGG CCC^GGG
SnaBI 4572 TACGTA TAC^GTA
Van91I 5109 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 50 CCCGGG C^CCGGG
XmaI 50 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 122 GTCTAC GT^[AC][GT]AC
2379 GTCTAC
AccIII 474 TCCGGA T^CCGGA
3730 TCCGGA
AocI 1519 CCTGAGG CC^T[ACGT]AGG
5379 CCTTAGG
AvaI 50 CCCGGG C^[CT]CG[GA]G
64 CTCGGG
AvrII 1338 CCTAGG C^CTAGG
1942 CCTAGG
BalI 1616 TGGCCA TGG^CCA
3783 TGGCCA
BciVI 252 GGATAC GTATCC(6/5)
1050 GGATAC
BcnI 50 CCCGG CC^[GC]GG
51 CCGGG
BlnI 1338 CCTAGG C^CTAGG
1942 CCTAGG
BsaWI 474 TCCGGA [AT]^CCGG[AT]
3730 TCCGGA
BseAI 474 TCCGGA T^CCGGA
3730 TCCGGA
BsmBI 1025 CGTCTC CGTCTC(1/5)
4377 CGTCTC
BsoBI 50 CCCGGG C^[CT]CG[GA]G
64 CTCGGG
Bsp1407I 972 TGTACA T^GTACA
3183 TGTACA
BspEI 474 TCCGGA T^CCGGA
3730 TCCGGA
BsrGI 972 TGTACA T^GTACA
3183 TGTACA
BssSI 5018 CACGAG CACGAG(-5/-1)
3313 CTCGTG
Bsu36I 1519 CCTGAGG CC^T[ACGT]AGG
5379 CCTTAGG
DsaI 6 CCATGG C^C[GA][CT]GG
5464 CCATGG
EaeI 1616 TGGCCA [CT]^GGCC[GA]
3783 TGGCCA
Eco81I 1519 CCTGAGG CC^T[ACGT]AGG
5379 CCTTAGG
HindIII 2004 AAGCTT A^AGCTT
4940 AAGCTT
MluNI 1616 TGGCCA TGG^CCA
3783 TGGCCA
MroI 474 TCCGGA T^CCGGA
3730 TCCGGA
MscI 1616 TGGCCA TGG^CCA
3783 TGGCCA
NciI 50 CCCGG CC^[GC]GG
51 CCGGG
NcoI 6 CCATGG C^CATGG
5464 CCATGG
NheI 1624 GCTAGC G^CTAGC
2243 GCTAGC
SapI 1182 GCTCTTC GCTCTTC(1/4)
2070 GCTCTTC
SpeI 706 ACTAGT A^CTAGT
4255 ACTAGT
SspBI 972 TGTACA T^GTACA
3183 TGTACA
SspI 5267 AATATT AAT^ATT
5394 AATATT
SwaI 5389 ATTTAAAT ATTT^AAAT
5409 ATTTAAAT
TaqI 286 TCGA T^CGA
1141 TCGA
TthHB8I 286 TCGA T^CGA
1141 TCGA
XbaI 3499 TCTAGA T^CTAGA
4973 TCTAGA
XcmI 1619 CCACAGCTAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4482 CCATAAAAGGCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI AseI AsnI
AspHI AvaII BanI BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspHI BspMI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII EarI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MnlI MseI MslI
MspA1I MspI MunI MvaI MwoI
NdeI NdeII NlaIII NlaIV NspI
PleI PshBI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII