##################################
# NO CUT ENZYMES ( 44 enzymes) #
##################################
AatII AccIII AclI AhdI Aor51HI
AscI AspEI BcgI BseAI BsiWI
BspDI BspEI BssHII BstBI ClaI
CpoI DrdI Eam1105I Eco47III EcoT22I
FseI KspI MroI NheI NotI
NruI NsiI NspV PacI PaeR7I
PmeI Psp1406I PvuI RsrII SacII
SalI SapI ScaI SfiI SfuI
SnaBI SphI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AcyI 2030 GGCGCC G[GA]^CG[CT]C
AflII 6768 CTTAAG C^TTAAG
AgeI 6728 ACCGGT A^CCGGT
ApaI 6106 GGGCCC GGGCC^C
AseI 4175 ATTAAT AT^TAAT
AsnI 4175 ATTAAT AT^TAAT
Asp700I 4065 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1924 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 248 CCCGGG C^[CT]CG[GA]G
AvrII 4468 CCTAGG C^CTAGG
BbeI 2030 GGCGCC GGCGC^C
BbrPI 6507 CACGTG CAC^GTG
BfrI 6768 CTTAAG C^TTAAG
BglI 1689 GCCGAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4468 CCTAGG C^CTAGG
BsaAI 6507 CACGTG [CT]AC^GT[GA]
BsaBI 1190 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2030 GGCGCC G[GA]^CG[CT]C
BsiEI 91 CGGCCG CG[GA][CT]^CG
BsmBI 4377 CGTCTC CGTCTC(1/5)
BsoBI 248 CCCGGG C^[CT]CG[GA]G
BsrBI 2724 GAGCGG CCGCTC(-3/-3)
Bst1107I 548 GTATAC GTA^TAC
BstEII 2912 GGTGACC G^GT[ACGT]ACC
BstPI 2912 GGTGACC G^GT[ACGT]ACC
BstZ17I 548 GTATAC GTA^TAC
EagI 91 CGGCCG C^GGCCG
EclXI 91 CGGCCG C^GGCCG
Eco52I 91 CGGCCG C^GGCCG
EcoO65I 2912 GGTGACC G^GT[ACGT]ACC
EcoRV 330 GATATC GAT^ATC
Hin1I 2030 GGCGCC G[GA]^CG[CT]C
KasI 2030 GGCGCC G^GCGCC
MamI 1190 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 6614 CAATTG C^AATTG
MluI 4 ACGCGT A^CGCGT
MunI 6614 CAATTG C^AATTG
NarI 2030 GGCGCC GG^CGCC
NdeI 5290 CATATG CA^TATG
PflFI 1924 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 6728 ACCGGT A^CCGGT
PmaCI 6507 CACGTG CAC^GTG
PmlI 6507 CACGTG CAC^GTG
PshAI 3027 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4175 ATTAAT AT^TAAT
SbfI 848 CCTGCAGG CCTGCA^GG
SexAI 1585 ACCTGGT A^CC[AT]GGT
SfoI 2030 GGCGCC GGC^GCC
SmaI 248 CCCGGG CCC^GGG
Sse8387I 848 CCTGCAGG CCTGCA^GG
Tth111I 1924 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5088 TCTAGA T^CTAGA
XmaCI 248 CCCGGG C^CCGGG
XmaI 248 CCCGGG C^CCGGG
XmnI 4065 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Acc65I 2431 GGTACC G^GTACC
2638 GGTACC
AccI 548 GTATAC GT^[AC][GT]AC
4679 GTCTAC
Alw44I 5544 GTGCAC G^TGCAC
6512 GTGCAC
ApaLI 5544 GTGCAC G^TGCAC
6512 GTGCAC
Asp718I 2431 GGTACC G^GTACC
2638 GGTACC
AviII 235 TGCGCA TGC^GCA
676 TGCGCA
BciVI 5621 GGATAC GTATCC(6/5)
3871 GTATCC
BsaWI 2458 TCCGGT [AT]^CCGG[AT]
6728 ACCGGT
BspHI 5131 TCATGA T^CATGA
5843 TCATGA
BspLU11I 3385 ACATGT A^CATGT
5731 ACATGT
DraI 4739 TTTAAA TTT^AAA
7053 TTTAAA
EcoNI 4339 CCTGCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4831 CCTTGGACAGG
EcoRI 3636 GAATTC G^AATTC
5441 GAATTC
FspI 235 TGCGCA TGC^GCA
676 TGCGCA
HpaI 5081 GTTAAC GTT^AAC
6931 GTTAAC
KpnI 2431 GGTACC GGTAC^C
2638 GGTACC
RcaI 5131 TCATGA T^CATGA
5843 TCATGA
SgrAI 95 CGCCGGCG C[GA]^CCGG[CT]G
555 CGCCGGCG
StuI 4336 AGGCCT AGG^CCT
4471 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaII BalI BamHI BanI
BanII BbsI BbvCI BbvI BclI
BcnI BfaI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspMI BsrDI BsrFI BsrGI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SpeI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII