##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccIII AclI AgeI Alw44I
ApaLI AscI AviII AvrII BamHI
BbrPI BlnI BlpI Bpu1102I BsaBI
BseAI BsiEI BsiWI BspDI BspEI
BssHII CelII ClaI CpoI DrdI
EagI EclXI Eco52I FseI FspI
HgaI HincII HindII HindIII HpaI
MamI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NotI NruI
PacI PaeR7I PinAI PmaCI PmlI
PshAI Psp1406I PvuI RsrII SacI
SalI SgrAI SnaBI SpeI SphI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 595 GGTACC G^GTACC
AccI 259 GTATAC GT^[AC][GT]AC
AflIII 684 ACATGT A^C[GA][CT]GT
AhdI 1890 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4900 AGCGCT AGC^GCT
AseI 5766 ATTAAT AT^TAAT
AsnI 5766 ATTAAT AT^TAAT
Asp700I 1532 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 595 GGTACC G^GTACC
AspEI 1890 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 5158 GCAGTGTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1447 GTATCC GTATCC(6/5)
BclI 6191 TGATCA T^GATCA
BglII 5584 AGATCT A^GATCT
BsmBI 1964 CGTCTC CGTCTC(1/5)
BspLU11I 684 ACATGT A^CATGT
BspMI 2498 GCAGGT ACCTGC(4/8)
BsrFI 946 ACCGGC [GA]^CCGG[CT]
Bst1107I 259 GTATAC GTA^TAC
BstBI 3376 TTCGAA TT^CGAA
BstZ17I 259 GTATAC GTA^TAC
Cfr10I 946 ACCGGC [GA]^CCGG[CT]
Eam1105I 1890 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4900 AGCGCT AGC^GCT
EcoRI 2506 GAATTC G^AATTC
EcoRV 3188 GATATC GAT^ATC
EcoT22I 1215 ATGCAT ATGCA^T
FbaI 6191 TGATCA T^GATCA
KpnI 595 GGTACC GGTAC^C
KspI 2152 CCGCGG CCGC^GG
NheI 1664 GCTAGC G^CTAGC
NsiI 1215 ATGCAT ATGCA^T
NspV 3376 TTCGAA TT^CGAA
PflMI 4123 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 5848 GTTTAAAC GTTT^AAAC
PshBI 5766 ATTAAT AT^TAAT
SacII 2152 CCGCGG CCGC^GG
SapI 4571 GAAGAGC GCTCTTC(1/4)
SfiI 1310 GGCCAGAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 3376 TTCGAA TT^CGAA
SmaI 1095 CCCGGG CCC^GGG
SspI 3356 AATATT AAT^ATT
StuI 6128 AGGCCT AGG^CCT
SwaI 5616 ATTTAAAT ATTT^AAAT
Van91I 4123 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1095 CCCGGG C^CCGGG
XmaI 1095 CCCGGG C^CCGGG
XmnI 1532 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 4632 GGCGCC G[GA]^CG[CT]C
4949 GGCGCC
AflII 5321 CTTAAG C^TTAAG
6401 CTTAAG
AspI 3512 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4351 GACAGAGTC
AvaI 1095 CCCGGG C^[CT]CG[GA]G
2469 CTCGGG
BbeI 4632 GGCGCC GGCGC^C
4949 GGCGCC
BfrI 5321 CTTAAG C^TTAAG
6401 CTTAAG
BsaAI 3542 CACGTA [CT]AC^GT[GA]
6298 TACGTG
BsaHI 4632 GGCGCC G[GA]^CG[CT]C
4949 GGCGCC
BsaWI 2400 ACCGGA [AT]^CCGG[AT]
4410 ACCGGA
BsgI 760 CTGCAC GTGCAG(16/14)
5257 GTGCAG
BsmI 67 GAATGC GAATGC(1/-1)
3152 GCATTC
BsoBI 1095 CCCGGG C^[CT]CG[GA]G
2469 CTCGGG
Bsp1407I 4208 TGTACA T^GTACA
6012 TGTACA
BsrGI 4208 TGTACA T^GTACA
6012 TGTACA
BssSI 5166 CTCGTG CACGAG(-5/-1)
5229 CTCGTG
BstEII 1544 GGTTACC G^GT[ACGT]ACC
4421 GGTGACC
BstPI 1544 GGTTACC G^GT[ACGT]ACC
4421 GGTGACC
DraIII 92 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
951 CACAAGGTG
EcoO65I 1544 GGTTACC G^GT[ACGT]ACC
4421 GGTGACC
Hin1I 4632 GGCGCC G[GA]^CG[CT]C
4949 GGCGCC
KasI 4632 GGCGCC G^GCGCC
4949 GGCGCC
NarI 4632 GGCGCC GG^CGCC
4949 GGCGCC
PflFI 3512 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4351 GACAGAGTC
SbfI 1525 CCTGCAGG CCTGCA^GG
4891 CCTGCAGG
ScaI 647 AGTACT AGT^ACT
1580 AGTACT
SfoI 4632 GGCGCC GGC^GCC
4949 GGCGCC
Sse8387I 1525 CCTGCAGG CCTGCA^GG
4891 CCTGCAGG
SspBI 4208 TGTACA T^GTACA
6012 TGTACA
TaqI 3377 TCGA T^CGA
4739 TCGA
Tth111I 3512 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4351 GACAGAGTC
TthHB8I 3377 TCGA T^CGA
4739 TCGA
XcmI 2914 CCATGAGACTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4119 CCACCCAGCCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BsrBI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII