##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AccIII AflII AgeI Alw44I
ApaLI AscI Asp718I AspI AviII
AvrII BbrPI BcgI BciVI BfrI
BglII BlnI BsaBI BsaWI BseAI
BsiWI BspEI BssSI BstBI BstEII
BstPI CpoI DrdI EcoO65I EcoRI
EcoRV FseI FspI HpaI KpnI
MamI MfeI MluI MroI MunI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PvuI RsrII SalI SbfI
ScaI SexAI SfuI SgrAI SnaBI
Sse8387I SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
AatII 603 GACGTC GACGT^C
AclI 6549 AACGTT AA^CGTT
Aor51HI 789 AGCGCT AGC^GCT
AseI 438 ATTAAT AT^TAAT
AsnI 438 ATTAAT AT^TAAT
BclI 875 TGATCA T^GATCA
BsmBI 135 CGTCTC CGTCTC(1/5)
Bsp1407I 2822 TGTACA T^GTACA
BspDI 171 ATCGAT AT^CGAT
BspLU11I 2825 ACATGT A^CATGT
BsrGI 2822 TGTACA T^GTACA
BssHII 2738 GCGCGC G^CGCGC
ClaI 171 ATCGAT AT^CGAT
Eco47III 789 AGCGCT AGC^GCT
EcoT22I 6354 ATGCAT ATGCA^T
FbaI 875 TGATCA T^GATCA
NdeI 6204 CATATG CA^TATG
NsiI 6354 ATGCAT ATGCA^T
PshBI 438 ATTAAT AT^TAAT
Psp1406I 6549 AACGTT AA^CGTT
SapI 3138 GAAGAGC GCTCTTC(1/4)
SpeI 5420 ACTAGT A^CTAGT
SspBI 2822 TGTACA T^GTACA
XbaI 2115 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
BamHI 4210 GGATCC G^GATCC
4960 GGATCC
BsmI 650 GCATTC GAATGC(1/-1)
2111 GCATTC
BspHI 878 TCATGA T^CATGA
4370 TCATGA
Bst1107I 5045 GTATAC GTA^TAC
6376 GTATAC
BstZ17I 5045 GTATAC GTA^TAC
6376 GTATAC
DraI 6779 TTTAAA TTT^AAA
6787 TTTAAA
NaeI 51 GCCGGC GCC^GGC
2497 GCCGGC
NgoMIV 51 GCCGGC G^CCGGC
2497 GCCGGC
NheI 6188 GCTAGC G^CTAGC
6675 GCTAGC
RcaI 878 TCATGA T^CATGA
4370 TCATGA
SfiI 41 GGCCCGGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1053 GGCCGCGCTGGCC
SphI 3014 GCATGC GCATG^C
3467 GCATGC
SspI 182 AATATT AAT^ATT
4925 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 157 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AhdI AluI AlwI
AlwNI AocI ApaI ApoI Asp700I
AspEI AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI