##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AclI AgeI ApaI
AscI AseI AsnI Asp718I AvrII
BbrPI BcgI BciVI BlnI BlpI
Bpu1102I BsiWI BsrDI BssHII Bst1107I
BstBI BstZ17I CelII CpoI DrdI
EcoRV FseI KpnI KspI MfeI
MluI MunI NaeI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I RsrII SacII
ScaI SexAI SfiI SfuI SgrAI
SpeI StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccIII 3359 TCCGGA T^CCGGA
AflII 1427 CTTAAG C^TTAAG
AhdI 110 GACTGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 515 AGCGCT AGC^GCT
AspEI 110 GACTGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1237 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1005 TGCGCA TGC^GCA
BbeI 4174 GGCGCC GGCGC^C
BbvCI 518 GCTGAGG CCTCAGC(-5/-2)
BfrI 1427 CTTAAG C^TTAAG
BglII 1265 AGATCT A^GATCT
BsaAI 1415 TACGTA [CT]AC^GT[GA]
BseAI 3359 TCCGGA T^CCGGA
BsmI 5106 GAATGC GAATGC(1/-1)
Bsp1407I 4399 TGTACA T^GTACA
BspDI 3928 ATCGAT AT^CGAT
BspEI 3359 TCCGGA T^CCGGA
BsrFI 3667 GCCGGT [GA]^CCGG[CT]
BsrGI 4399 TGTACA T^GTACA
BstEII 3887 GGTGACC G^GT[ACGT]ACC
BstPI 3887 GGTGACC G^GT[ACGT]ACC
Cfr10I 3667 GCCGGT [GA]^CCGG[CT]
ClaI 3928 ATCGAT AT^CGAT
DraIII 3343 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 110 GACTGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 515 AGCGCT AGC^GCT
EcoO65I 3887 GGTGACC G^GT[ACGT]ACC
EcoRI 2538 GAATTC G^AATTC
EcoT22I 1647 ATGCAT ATGCA^T
FspI 1005 TGCGCA TGC^GCA
HpaI 4713 GTTAAC GTT^AAC
KasI 4174 GGCGCC G^GCGCC
MroI 3359 TCCGGA T^CCGGA
NarI 4174 GGCGCC GG^CGCC
NcoI 1635 CCATGG C^CATGG
NsiI 1647 ATGCAT ATGCA^T
PflFI 1237 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2896 CCATCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 1762 CGATCG CGAT^CG
SalI 1397 GTCGAC G^TCGAC
SbfI 3535 CCTGCAGG CCTGCA^GG
SfoI 4174 GGCGCC GGC^GCC
SmaI 2555 CCCGGG CCC^GGG
SnaBI 1415 TACGTA TAC^GTA
SphI 2985 GCATGC GCATG^C
Sse8387I 3535 CCTGCAGG CCTGCA^GG
SspBI 4399 TGTACA T^GTACA
SspI 4830 AATATT AAT^ATT
Tth111I 1237 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2896 CCATCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3997 TCTAGA T^CTAGA
XmaCI 2555 CCCGGG C^CCGGG
XmaI 2555 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Alw44I 4136 GTGCAC G^TGCAC
5308 GTGCAC
AocI 3489 CCTCAGG CC^T[ACGT]AGG
3824 CCTCAGG
ApaLI 4136 GTGCAC G^TGCAC
5308 GTGCAC
BamHI 3266 GGATCC G^GATCC
4042 GGATCC
BclI 1945 TGATCA T^GATCA
3294 TGATCA
BsaWI 3359 TCCGGA [AT]^CCGG[AT]
3701 ACCGGA
BsmBI 2920 CGTCTC CGTCTC(1/5)
3201 CGTCTC
BspLU11I 1703 ACATGT A^CATGT
2218 ACATGT
BstAPI 2976 GCATCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4756 GCATGATTTGC
Bsu36I 3489 CCTCAGG CC^T[ACGT]AGG
3824 CCTCAGG
EagI 3051 CGGCCG C^GGCCG
4226 CGGCCG
EclXI 3051 CGGCCG C^GGCCG
4226 CGGCCG
Eco52I 3051 CGGCCG C^GGCCG
4226 CGGCCG
Eco81I 3489 CCTCAGG CC^T[ACGT]AGG
3824 CCTCAGG
FbaI 1945 TGATCA T^GATCA
3294 TGATCA
HindIII 2003 AAGCTT A^AGCTT
5534 AAGCTT
PpuMI 995 AGGTCCT [GA]G^G[AT]CC[CT]
3965 GGGTCCC
SacI 138 GAGCTC GAGCT^C
1898 GAGCTC
SapI 956 GAAGAGC GCTCTTC(1/4)
4166 GAAGAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaBI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrBI BsrI BssKI
BssSI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI