##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AccII AclI AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI AviII
BbeI BbrPI BcgI BciVI BsaAI
BsiEI BsiWI BsmBI BspDI BspHI
BssHII BstAPI BstBI BstUI ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I FseI FspI HpaI KasI
KspI MfeI MluI MunI MvnI
NaeI NarI NgoMIV NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SbfI
SfoI SfuI SgrAI SnaBI Sse8387I
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 3724 GACGTC GACGT^C
Acc65I 2103 GGTACC G^GTACC
AccIII 3117 TCCGGA T^CCGGA
AhdI 2162 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 2103 GGTACC G^GTACC
AspEI 2162 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4709 CCTAGG C^CTAGG
BlnI 4709 CCTAGG C^CTAGG
BsaBI 5731 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2527 GGTCTC GGTCTC(1/5)
BseAI 3117 TCCGGA T^CCGGA
BsgI 4895 GTGCAG GTGCAG(16/14)
BspEI 3117 TCCGGA T^CCGGA
BssSI 2556 CACGAG CACGAG(-5/-1)
CfoI 2791 GCGC GCG^C
DraIII 2954 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2162 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 61 GAATTC G^AATTC
EcoT22I 6642 ATGCAT ATGCA^T
HaeII 2790 AGCGCC [GA]GCGC^[CT]
HhaI 2791 GCGC GCG^C
HinP1I 2791 GCGC G^CGC
KpnI 2103 GGTACC GGTAC^C
MamI 5731 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3117 TCCGGA T^CCGGA
NheI 1067 GCTAGC G^CTAGC
NsiI 6642 ATGCAT ATGCA^T
SalI 2073 GTCGAC G^TCGAC
ScaI 2154 AGTACT AGT^ACT
SexAI 1884 ACCTGGT A^CC[AT]GGT
SfiI 2041 GGCCTCTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 3896 ACTAGT A^CTAGT
XbaI 7018 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 1307 GACGCC G[GA]^CG[CT]C
3724 GACGTC
AflII 245 CTTAAG C^TTAAG
3876 CTTAAG
BalI 4166 TGGCCA TGG^CCA
6746 TGGCCA
BclI 3048 TGATCA T^GATCA
7122 TGATCA
BfrI 245 CTTAAG C^TTAAG
3876 CTTAAG
BglII 340 AGATCT A^GATCT
1092 AGATCT
BsaHI 1307 GACGCC G[GA]^CG[CT]C
3724 GACGTC
BsmI 7022 GAATGC GAATGC(1/-1)
5138 GCATTC
BsrFI 1713 ACCGGC [GA]^CCGG[CT]
2766 ACCGGC
Bst1107I 6035 GTATAC GTA^TAC
7143 GTATAC
BstZ17I 6035 GTATAC GTA^TAC
7143 GTATAC
Cfr10I 1713 ACCGGC [GA]^CCGG[CT]
2766 ACCGGC
EcoNI 2029 CCTAAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3820 CCTTTCCCAGG
EcoRV 1535 GATATC GAT^ATC
4018 GATATC
FbaI 3048 TGATCA T^GATCA
7122 TGATCA
Hin1I 1307 GACGCC G[GA]^CG[CT]C
3724 GACGTC
HindIII 4313 AAGCTT A^AGCTT
6153 AAGCTT
MluNI 4166 TGGCCA TGG^CCA
6746 TGGCCA
MscI 4166 TGGCCA TGG^CCA
6746 TGGCCA
PstI 5063 CTGCAG CTGCA^G
5975 CTGCAG
SacI 6909 GAGCTC GAGCT^C
7037 GAGCTC
SapI 5316 GAAGAGC GCTCTTC(1/4)
756 GCTCTTC
SmaI 1978 CCCGGG CCC^GGG
6299 CCCGGG
SphI 2448 GCATGC GCATG^C
2623 GCATGC
SspI 3174 AATATT AAT^ATT
5028 AATATT
XcmI 334 CCATGCAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
6074 CCAAGATGCAACTGG
XmaCI 1978 CCCGGG C^CCGGG
6299 CCCGGG
XmaI 1978 CCCGGG C^CCGGG
6299 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
ApoI Asp700I AspHI AspI AvaI
AvaII BamHI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspLU11I BspMI BsrBI BsrDI
BsrGI BsrI BssKI BstEII BstF5I
BstNI BstPI BstXI BstYI Bsu36I
Cac8I CelII Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XhoII XmnI