##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
AccIII AclI AscI AseI AsnI
BciVI BseAI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI EcoRV
EcoT22I FseI MluI MroI NheI
NotI NruI NsiI NspV PacI
PaeR7I PmeI PshBI Psp1406I PvuI
RsrII SalI ScaI SfuI SnaBI
SpeI SspBI SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 5418 GACGTC GACGT^C
Acc65I 6116 GGTACC G^GTACC
AccI 1621 GTCTAC GT^[AC][GT]AC
AflIII 378 ACGTGT A^C[GA][CT]GT
AgeI 7472 ACCGGT A^CCGGT
AhdI 6380 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3270 AGCGCT AGC^GCT
Asp700I 4215 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 6116 GGTACC G^GTACC
AspEI 6380 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 3122 GGCGCC GGCGC^C
BcgI 5089 CGACACTATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 7208 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 6688 CGGCCG CG[GA][CT]^CG
BsmI 2904 GCATTC GAATGC(1/-1)
BspHI 6878 TCATGA T^CATGA
BstEII 7043 GGTGACC G^GT[ACGT]ACC
BstPI 7043 GGTGACC G^GT[ACGT]ACC
DraI 7561 TTTAAA TTT^AAA
DrdI 2247 GACCACAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 6688 CGGCCG C^GGCCG
Eam1105I 6380 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 6688 CGGCCG C^GGCCG
Eco47III 3270 AGCGCT AGC^GCT
Eco52I 6688 CGGCCG C^GGCCG
EcoO65I 7043 GGTGACC G^GT[ACGT]ACC
EcoRI 3459 GAATTC G^AATTC
HincII 613 GTTAAC GT[CT]^[GA]AC
HindII 613 GTTAAC GT[CT]^[GA]AC
HindIII 164 AAGCTT A^AGCTT
HpaI 613 GTTAAC GTT^AAC
KasI 3122 GGCGCC G^GCGCC
KpnI 6116 GGTACC GGTAC^C
KspI 6666 CCGCGG CCGC^GG
MamI 7208 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4678 CAATTG C^AATTG
MunI 4678 CAATTG C^AATTG
NarI 3122 GGCGCC GG^CGCC
NdeI 700 CATATG CA^TATG
PinAI 7472 ACCGGT A^CCGGT
PshAI 1614 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 6878 TCATGA T^CATGA
SacII 6666 CCGCGG CCGC^GG
SapI 7407 GCTCTTC GCTCTTC(1/4)
SexAI 5315 ACCAGGT A^CC[AT]GGT
SfoI 3122 GGCGCC GGC^GCC
SphI 1284 GCATGC GCATG^C
XmnI 4215 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 3459 GAATTC [GA]^AATT[CT]
7597 AAATTT
AflII 134 CTTAAG C^TTAAG
5578 CTTAAG
Alw44I 170 GTGCAC G^TGCAC
1463 GTGCAC
ApaLI 170 GTGCAC G^TGCAC
1463 GTGCAC
ApoI 3459 GAATTC [GA]^AATT[CT]
7597 AAATTT
AviII 1943 TGCGCA TGC^GCA
6497 TGCGCA
BamHI 6470 GGATCC G^GATCC
7146 GGATCC
BclI 72 TGATCA T^GATCA
6614 TGATCA
BfrI 134 CTTAAG C^TTAAG
5578 CTTAAG
BglII 915 AGATCT A^GATCT
7320 AGATCT
BsaWI 5797 TCCGGT [AT]^CCGG[AT]
7472 ACCGGT
BsmBI 781 GAGACG CGTCTC(1/5)
4387 GAGACG
BsrDI 1963 CATTGC GCAATG(2/0)
7109 CATTGC
BssSI 631 CACGAG CACGAG(-5/-1)
4384 CACGAG
FbaI 72 TGATCA T^GATCA
6614 TGATCA
FspI 1943 TGCGCA TGC^GCA
6497 TGCGCA
SbfI 5232 CCTGCAGG CCTGCA^GG
6993 CCTGCAGG
SfiI 4609 GGCCAGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
6964 GGCCTCCGGGGCC
Sse8387I 5232 CCTGCAGG CCTGCA^GG
6993 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AspI AvaI AvaII AvrII BalI
BanI BanII BbrPI BbsI BbvCI
BbvI BcnI BfaI BglI BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SgrAI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmaCI XmaI