##################################
# NO CUT ENZYMES ( 35 enzymes) #
##################################
AatII AccI AclI AscI BsiWI
BspDI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI FseI KspI MluI
NotI NruI NspV PacI PmeI
Psp1406I PvuI RsrII SacII SalI
SbfI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AflII 655 CTTAAG C^TTAAG
AgeI 1124 ACCGGT A^CCGGT
AhdI 6763 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 5668 AGCGCT AGC^GCT
AseI 7162 ATTAAT AT^TAAT
AsnI 7162 ATTAAT AT^TAAT
AspEI 6763 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2119 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 5207 TGCGCA TGC^GCA
AvrII 6571 CCTAGG C^CTAGG
BbeI 6636 GGCGCC GGCGC^C
BbrPI 666 CACGTG CAC^GTG
BcgI 774 GCAGCTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 655 CTTAAG C^TTAAG
BlnI 6571 CCTAGG C^CTAGG
BsaI 3181 GAGACC GGTCTC(1/5)
BsrBI 379 GAGCGG CCGCTC(-3/-3)
BstEII 25 GGTCACC G^GT[ACGT]ACC
BstPI 25 GGTCACC G^GT[ACGT]ACC
DraI 7111 TTTAAA TTT^AAA
DraIII 7725 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 7688 GACAAGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 5706 CGGCCG C^GGCCG
Eam1105I 6763 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 5706 CGGCCG C^GGCCG
Eco47III 5668 AGCGCT AGC^GCT
Eco52I 5706 CGGCCG C^GGCCG
EcoO65I 25 GGTCACC G^GT[ACGT]ACC
EcoRV 886 GATATC GAT^ATC
EcoT22I 178 ATGCAT ATGCA^T
FspI 5207 TGCGCA TGC^GCA
HpaI 3292 GTTAAC GTT^AAC
KasI 6636 GGCGCC G^GCGCC
MfeI 8025 CAATTG C^AATTG
MunI 8025 CAATTG C^AATTG
NarI 6636 GGCGCC GG^CGCC
NdeI 578 CATATG CA^TATG
NheI 4419 GCTAGC G^CTAGC
NsiI 178 ATGCAT ATGCA^T
PflFI 2119 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1124 ACCGGT A^CCGGT
PmaCI 666 CACGTG CAC^GTG
PmlI 666 CACGTG CAC^GTG
PshBI 7162 ATTAAT AT^TAAT
SapI 3105 GCTCTTC GCTCTTC(1/4)
SfiI 5685 GGCCCGCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 6636 GGCGCC GGC^GCC
Tth111I 2119 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2817 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 3942 GGTACC G^GTACC
4796 GGTACC
AccIII 2582 TCCGGA T^CCGGA
4605 TCCGGA
Asp718I 3942 GGTACC G^GTACC
4796 GGTACC
BalI 3759 TGGCCA TGG^CCA
6865 TGGCCA
BamHI 1853 GGATCC G^GATCC
3560 GGATCC
BclI 4202 TGATCA T^GATCA
4479 TGATCA
BlpI 1769 GCTCAGC GC^T[ACGT]AGC
4248 GCTAAGC
Bpu1102I 1769 GCTCAGC GC^T[ACGT]AGC
4248 GCTAAGC
BsaBI 2973 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5104 GATCCCAATC
BseAI 2582 TCCGGA T^CCGGA
4605 TCCGGA
BsiEI 1488 CGACCG CG[GA][CT]^CG
5706 CGGCCG
BsmBI 4818 CGTCTC CGTCTC(1/5)
6383 CGTCTC
BspEI 2582 TCCGGA T^CCGGA
4605 TCCGGA
CelII 1769 GCTCAGC GC^T[ACGT]AGC
4248 GCTAAGC
FbaI 4202 TGATCA T^GATCA
4479 TGATCA
HincII 3292 GTTAAC GT[CT]^[GA]AC
3973 GTCAAC
HindII 3292 GTTAAC GT[CT]^[GA]AC
3973 GTCAAC
KpnI 3942 GGTACC GGTAC^C
4796 GGTACC
MamI 2973 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5104 GATCCCAATC
MluNI 3759 TGGCCA TGG^CCA
6865 TGGCCA
MroI 2582 TCCGGA T^CCGGA
4605 TCCGGA
MscI 3759 TGGCCA TGG^CCA
6865 TGGCCA
NaeI 191 GCCGGC GCC^GGC
5577 GCCGGC
NgoMIV 191 GCCGGC G^CCGGC
5577 GCCGGC
PaeR7I 5630 CTCGAG C^TCGAG
7151 CTCGAG
PshAI 2937 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3201 GACCTCAGTC
SexAI 110 ACCTGGT A^CC[AT]GGT
7451 ACCAGGT
SphI 2084 GCATGC GCATG^C
6346 GCATGC
XhoI 5630 CTCGAG C^TCGAG
7151 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BciVI BcnI
BfaI BglI BglII BmyI BpmI
BpuAI BsaAI BsaHI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspHI BspLU11I BspMI BsrDI
BsrFI BsrGI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspBI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmnI