##################################
# NO CUT ENZYMES ( 35 enzymes) #
##################################
AclI AgeI Alw44I ApaLI AscI
AviII BsiWI BspDI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI EcoT22I
FseI FspI MfeI MluI MunI
NdeI NotI NruI NsiI NspV
PinAI PmeI Psp1406I RsrII SalI
SexAI SfiI SfuI SgrAI SwaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 3425 GACGTC GACGT^C
Acc65I 2921 GGTACC G^GTACC
Asp718I 2921 GGTACC G^GTACC
AspI 8670 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 250 CCTAGG C^CTAGG
BbrPI 5199 CACGTG CAC^GTG
BlnI 250 CCTAGG C^CTAGG
BspLU11I 10001 ACATGT A^CATGT
BstAPI 10202 GCATGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI 2818 CGGCCG C^GGCCG
EclXI 2818 CGGCCG C^GGCCG
Eco52I 2818 CGGCCG C^GGCCG
EcoRV 7277 GATATC GAT^ATC
HindIII 9029 AAGCTT A^AGCTT
HpaI 1669 GTTAAC GTT^AAC
KpnI 2921 GGTACC GGTAC^C
KspI 2707 CCGCGG CCGC^GG
NcoI 5502 CCATGG C^CATGG
NheI 5492 GCTAGC G^CTAGC
PacI 10950 TTAATTAA TTAAT^TAA
PflFI 8670 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2591 CCAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 5199 CACGTG CAC^GTG
PmlI 5199 CACGTG CAC^GTG
PshAI 6328 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 6631 CGATCG CGAT^CG
SacII 2707 CCGCGG CCGC^GG
SbfI 454 CCTGCAGG CCTGCA^GG
ScaI 2988 AGTACT AGT^ACT
SnaBI 9559 TACGTA TAC^GTA
Sse8387I 454 CCTGCAGG CCTGCA^GG
SspI 8840 AATATT AAT^ATT
Tth111I 8670 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2591 CCAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 1977 TCCGGA T^CCGGA
2367 TCCGGA
AflII 2086 CTTAAG C^TTAAG
10074 CTTAAG
AflIII 5198 ACACGT A^C[GA][CT]GT
10001 ACATGT
Asp700I 2342 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5996 GAAATGCTTC
BamHI 71 GGATCC G^GATCC
1974 GGATCC
BbeI 6469 GGCGCC GGCGC^C
8414 GGCGCC
BciVI 9179 GGATAC GTATCC(6/5)
3519 GTATCC
BfrI 2086 CTTAAG C^TTAAG
10074 CTTAAG
BglII 588 AGATCT A^GATCT
1690 AGATCT
BsaAI 5199 CACGTG [CT]AC^GT[GA]
9559 TACGTA
BsaBI 4305 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
8181 GATCGAAATC
BsaWI 1977 TCCGGA [AT]^CCGG[AT]
2367 TCCGGA
BseAI 1977 TCCGGA T^CCGGA
2367 TCCGGA
BsiEI 2818 CGGCCG CG[GA][CT]^CG
6631 CGATCG
BspEI 1977 TCCGGA T^CCGGA
2367 TCCGGA
DraIII 2060 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
8339 CACAGGGTG
KasI 6469 GGCGCC G^GCGCC
8414 GGCGCC
MamI 4305 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
8181 GATCGAAATC
MroI 1977 TCCGGA T^CCGGA
2367 TCCGGA
NaeI 2820 GCCGGC GCC^GGC
7607 GCCGGC
NarI 6469 GGCGCC GG^CGCC
8414 GGCGCC
NgoMIV 2820 GCCGGC G^CCGGC
7607 GCCGGC
SfoI 6469 GGCGCC GGC^GCC
8414 GGCGCC
SpeI 9742 ACTAGT A^CTAGT
11247 ACTAGT
SphI 7854 GCATGC GCATG^C
10800 GCATGC
XbaI 1228 TCTAGA T^CTAGA
4958 TCTAGA
XmnI 2342 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5996 GAAATGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 162 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AhdI AluI AlwI AlwNI
AocI Aor51HI ApaI ApoI AseI
AsnI AspEI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcgI BclI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssKI BssSI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DrdI DsaI EaeI
Eam1105I EarI Eco47III Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PaeR7I
PleI PpuMI PshBI PstI PvuII
RcaI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoI XhoII
XmaCI XmaI