##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
Acc65I AccII AflII AscI Asp718I
AviII BbeI BfrI BsaBI BsrBI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstZ17I CpoI EagI EclXI
Eco52I EcoO65I EcoRV FseI FspI
HpaI KasI KpnI KspI MamI
MluI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PmeI PvuI RsrII SacII
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI Sse8387I SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 6041 GACGTC GACGT^C
AccIII 2988 TCCGGA T^CCGGA
AcyI 6041 GACGTC G[GA]^CG[CT]C
AgeI 1017 ACCGGT A^CCGGT
AhdI 268 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 8192 GTGCAC G^TGCAC
ApaLI 8192 GTGCAC G^TGCAC
AseI 7388 ATTAAT AT^TAAT
AsnI 7388 ATTAAT AT^TAAT
AspEI 268 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2134 CCTAGG C^CTAGG
BcgI 7009 CGAGGATAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 2134 CCTAGG C^CTAGG
BsaHI 6041 GACGTC G[GA]^CG[CT]C
BseAI 2988 TCCGGA T^CCGGA
BsiEI 1253 CGACCG CG[GA][CT]^CG
BsiWI 6603 CGTACG C^GTACG
Bsp1407I 64 TGTACA T^GTACA
BspEI 2988 TCCGGA T^CCGGA
BsrGI 64 TGTACA T^GTACA
BstBI 8961 TTCGAA TT^CGAA
DraIII 3214 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3417 GACATGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 268 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 1012 GCGGG CCCGC(4/6)
Hin1I 6041 GACGTC G[GA]^CG[CT]C
MfeI 3728 CAATTG C^AATTG
MroI 2988 TCCGGA T^CCGGA
MunI 3728 CAATTG C^AATTG
NspV 8961 TTCGAA TT^CGAA
PinAI 1017 ACCGGT A^CCGGT
PshAI 6057 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 7388 ATTAAT AT^TAAT
SacI 3092 GAGCTC GAGCT^C
SalI 6044 GTCGAC G^TCGAC
SfuI 8961 TTCGAA TT^CGAA
SspBI 64 TGTACA T^GTACA
XbaI 9241 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AclI 3619 AACGTT AA^CGTT
8163 AACGTT
Aor51HI 3273 AGCGCT AGC^GCT
4090 AGCGCT
AvaI 3324 CTCGGG C^[CT]CG[GA]G
7469 CCCGAG
BciVI 8215 GGATAC GTATCC(6/5)
10290 GTATCC
BglI 3155 GCCTTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
8087 GCCCAGTTGGC
BsoBI 3324 CTCGGG C^[CT]CG[GA]G
7469 CCCGAG
BspDI 371 ATCGAT AT^CGAT
9008 ATCGAT
ClaI 371 ATCGAT AT^CGAT
9008 ATCGAT
Eco47III 3273 AGCGCT AGC^GCT
4090 AGCGCT
EcoT22I 7052 ATGCAT ATGCA^T
8280 ATGCAT
NsiI 7052 ATGCAT ATGCA^T
8280 ATGCAT
Psp1406I 3619 AACGTT AA^CGTT
8163 AACGTT
#########################################
# 3 OR MORE CUT ENZYMES ( 151 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
ApoI Asp700I AspHI AspI AvaII
BalI BamHI BanI BanII BbrPI
BbsI BbvCI BbvI BclI BcnI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
Bsp1286I BspHI BspLU11I BspMI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRI
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RcaI RsaI
SapI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SpeI SphI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmnI