##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AatII AclI AflII AhdI AscI
AspEI AvrII BbrPI BclI BfrI
BlnI BsaAI BsiWI BspLU11I Bst1107I
BstZ17I DraI DrdI Eam1105I EcoT22I
FbaI HincII HindII HpaI MfeI
MluI MunI NotI NruI NsiI
PacI PmaCI PmeI PmlI PshAI
Psp1406I SalI SbfI SexAI SfiI
SgrAI SnaBI SpeI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 2629 GGTACC G^GTACC
AccI 294 GTCTAC GT^[AC][GT]AC
AflIII 3019 ACGTGT A^C[GA][CT]GT
Alw44I 2052 GTGCAC G^TGCAC
ApaLI 2052 GTGCAC G^TGCAC
AseI 345 ATTAAT AT^TAAT
AsnI 345 ATTAAT AT^TAAT
Asp718I 2629 GGTACC G^GTACC
AspI 6464 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 5096 TGCGCA TGC^GCA
BamHI 122 GGATCC G^GATCC
BcgI 4493 CGAGCGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 899 GGATAC GTATCC(6/5)
BsaBI 1967 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 3690 GAATGC GAATGC(1/-1)
Bsp1407I 611 TGTACA T^GTACA
BspHI 1825 TCATGA T^CATGA
BsrGI 611 TGTACA T^GTACA
BssHII 3383 GCGCGC G^CGCGC
BssSI 1909 CACGAG CACGAG(-5/-1)
CpoI 2634 CGGTCCG CG^G[AT]CCG
EagI 5626 CGGCCG C^GGCCG
EclXI 5626 CGGCCG C^GGCCG
Eco52I 5626 CGGCCG C^GGCCG
EcoRV 402 GATATC GAT^ATC
FseI 5708 GGCCGGCC GGCCGG^CC
FspI 5096 TGCGCA TGC^GCA
KpnI 2629 GGTACC GGTAC^C
KspI 5764 CCGCGG CCGC^GG
MamI 1967 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 2351 GCTAGC G^CTAGC
PflFI 6464 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 345 ATTAAT AT^TAAT
PvuI 3943 CGATCG CGAT^CG
RcaI 1825 TCATGA T^CATGA
RsrII 2634 CGGTCCG CG^G[AT]CCG
SacI 6520 GAGCTC GAGCT^C
SacII 5764 CCGCGG CCGC^GG
ScaI 3297 AGTACT AGT^ACT
SspBI 611 TGTACA T^GTACA
Tth111I 6464 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5387 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Aor51HI 4495 AGCGCT AGC^GCT
4796 AGCGCT
BbsI 1218 GAAGAC GAAGAC(2/6)
1323 GAAGAC
BbvCI 1936 CCTCAGC CCTCAGC(-5/-2)
5866 CCTCAGC
BglI 282 GCCTATGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3433 GCCAGGGTGGC
BglII 2117 AGATCT A^GATCT
2334 AGATCT
BlpI 3038 GCTCAGC GC^T[ACGT]AGC
6057 GCTCAGC
Bpu1102I 3038 GCTCAGC GC^T[ACGT]AGC
6057 GCTCAGC
BpuAI 1218 GAAGAC GAAGAC(2/6)
1323 GAAGAC
BspDI 1063 ATCGAT AT^CGAT
3600 ATCGAT
BspMI 4153 ACCTGC ACCTGC(4/8)
4359 ACCTGC
BstBI 226 TTCGAA TT^CGAA
367 TTCGAA
CelII 3038 GCTCAGC GC^T[ACGT]AGC
6057 GCTCAGC
ClaI 1063 ATCGAT AT^CGAT
3600 ATCGAT
Eco47III 4495 AGCGCT AGC^GCT
4796 AGCGCT
EcoNI 719 CCTTTACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3347 CCTGCTGAAGG
HindIII 4350 AAGCTT A^AGCTT
4423 AAGCTT
NdeI 1045 CATATG CA^TATG
1182 CATATG
NspV 226 TTCGAA TT^CGAA
367 TTCGAA
PaeR7I 5063 CTCGAG C^TCGAG
5703 CTCGAG
PvuII 3676 CAGCTG CAG^CTG
4732 CAGCTG
SapI 3890 GAAGAGC GCTCTTC(1/4)
3360 GCTCTTC
SfuI 226 TTCGAA TT^CGAA
367 TTCGAA
SmaI 3213 CCCGGG CCC^GGG
5141 CCCGGG
SphI 2091 GCATGC GCATG^C
6013 GCATGC
SspI 589 AATATT AAT^ATT
1390 AATATT
XhoI 5063 CTCGAG C^TCGAG
5703 CTCGAG
XmaCI 3213 CCCGGG C^CCGGG
5141 CCCGGG
XmaI 3213 CCCGGG C^CCGGG
5141 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AgeI AluI AlwI AlwNI
AocI ApaI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbeI BbvI BcnI BfaI BmyI
BpmI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspEI BsrBI BsrDI
BsrFI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoO109I
EcoO65I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MroI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PinAI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI