##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AccIII AclI AgeI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BciVI BclI
BglI BlnI BsaWI BseAI BsiWI
BsmBI BsoBI BspDI BspEI BsrBI
BsrFI BssHII Cfr10I ClaI CpoI
DrdI Eco47III FbaI FseI FspI
HaeII KasI KpnI KspI MluI
MroI NaeI NarI NgoMIV NotI
NruI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI Tth111I
Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 36 GACGTC GACGT^C
AccII 33 CGCG CG^CG
AcyI 36 GACGTC G[GA]^CG[CT]C
AhdI 36 GACGTCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 36 GACGTCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 4175 TGGCCA TGG^CCA
BanII 4222 GAGCTC G[GA]GC[CT]^C
BcgI 2544 CGACACCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 940 AGATCT A^GATCT
BlpI 5153 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 5153 GCTAAGC GC^T[ACGT]AGC
BsaAI 4389 TACGTG [CT]AC^GT[GA]
BsaHI 36 GACGTC G[GA]^CG[CT]C
BsiEI 6 CGGCCG CG[GA][CT]^CG
BsmI 151 GCATTC GAATGC(1/-1)
Bsp1407I 341 TGTACA T^GTACA
BspHI 3377 TCATGA T^CATGA
BsrGI 341 TGTACA T^GTACA
BssSI 3450 CTCGTG CACGAG(-5/-1)
BstAPI 3855 GCATATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3943 TTCGAA TT^CGAA
BstUI 33 CGCG CG^CG
BstXI 1046 CCAGCCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 5153 GCTAAGC GC^T[ACGT]AGC
DraIII 1426 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 6 CGGCCG C^GGCCG
Eam1105I 36 GACGTCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 6 CGGCCG C^GGCCG
Eco52I 6 CGGCCG C^GGCCG
EcoRI 3772 GAATTC G^AATTC
FauI 5529 GCGGG CCCGC(4/6)
HgaI 1386 GCGTC GACGC(5/10)
Hin1I 36 GACGTC G[GA]^CG[CT]C
HpaI 3204 GTTAAC GTT^AAC
MluNI 4175 TGGCCA TGG^CCA
MscI 4175 TGGCCA TGG^CCA
MvnI 33 CGCG CG^CG
NheI 4319 GCTAGC G^CTAGC
NspV 3943 TTCGAA TT^CGAA
PshAI 2940 GACTTTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3377 TCATGA T^CATGA
SacI 4222 GAGCTC GAGCT^C
SapI 17 GCTCTTC GCTCTTC(1/4)
SexAI 2816 ACCAGGT A^CC[AT]GGT
SfuI 3943 TTCGAA TT^CGAA
SspBI 341 TGTACA T^GTACA
XbaI 2615 TCTAGA T^CTAGA
XcmI 6297 CCAAAGACAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AflII 1299 CTTAAG C^TTAAG
2709 CTTAAG
AocI 2751 CCTCAGG CC^T[ACGT]AGG
3579 CCTAAGG
Asp700I 3408 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5584 GAACATATTC
AspHI 4222 GAGCTC G[AT]GC[AT]^C
4774 GTGCTC
BcnI 4 CCCGG CC^[GC]GG
9 CCGGG
BfrI 1299 CTTAAG C^TTAAG
2709 CTTAAG
BsaBI 4113 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5423 GATTATAATC
BsiHKAI 4222 GAGCTC G[AT]GC[AT]^C
4774 GTGCTC
BspMI 1155 ACCTGC ACCTGC(4/8)
240 GCAGGT
BstEII 2113 GGTTACC G^GT[ACGT]ACC
3121 GGTTACC
BstPI 2113 GGTTACC G^GT[ACGT]ACC
3121 GGTTACC
Bsu36I 2751 CCTCAGG CC^T[ACGT]AGG
3579 CCTAAGG
DraII 2236 AGGACCT [GA]G^G[ACGT]CC[CT]
4800 GGGACCT
EaeI 6 CGGCCG [CT]^GGCC[GA]
4175 TGGCCA
Eco81I 2751 CCTCAGG CC^T[ACGT]AGG
3579 CCTAAGG
EcoNI 5231 CCTTATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5769 CCTGAAACAGG
EcoO109I 2236 AGGACCT [GA]G^G[ACGT]CC[CT]
4800 GGGACCT
EcoO65I 2113 GGTTACC G^GT[ACGT]ACC
3121 GGTTACC
EcoRV 184 GATATC GAT^ATC
4395 GATATC
EcoT14I 4612 CCATGG C^C[AT][AT]GG
4793 CCATGG
EcoT22I 349 ATGCAT ATGCA^T
5649 ATGCAT
HapII 5 CCGG C^CGG
9 CCGG
HincII 3204 GTTAAC GT[CT]^[GA]AC
3936 GTTGAC
HindII 3204 GTTAAC GT[CT]^[GA]AC
3936 GTTGAC
HindIII 3786 AAGCTT A^AGCTT
4985 AAGCTT
HpaII 5 CCGG C^CGG
9 CCGG
MaeII 37 ACGT A^CGT
4390 ACGT
MamI 4113 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5423 GATTATAATC
MspI 5 CCGG C^CGG
9 CCGG
NciI 4 CCCGG CC^[GC]GG
9 CCGGG
NcoI 4612 CCATGG C^CATGG
4793 CCATGG
NsiI 349 ATGCAT ATGCA^T
5649 ATGCAT
PpuMI 2236 AGGACCT [GA]G^G[AT]CC[CT]
4800 GGGACCT
PstI 238 CTGCAG CTGCA^G
1157 CTGCAG
StyI 4612 CCATGG C^C[AT][AT]GG
4793 CCATGG
XmnI 3408 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5584 GAACATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI AvaII BbsI BbvCI BbvI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspLU11I BsrDI
BsrI BssKI Bst1107I BstF5I BstNI
BstYI BstZ17I Cac8I CfoI Cfr13I
DdeI DpnII DraI DsaI EarI
EcoRII Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MfeI
MflI MnlI MseI MslI MspA1I
MunI MvaI MwoI NdeI NdeII
NlaIII NlaIV NspI PleI PshBI
PvuII RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspI
SwaI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII